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Improved survival of laboratory-reared tsetse flies Glossina morsitans morsitans (Diptera: Glossinidae) through use of homidium bromide-treated blood diet

Published online by Cambridge University Press:  01 September 2010

James Karuku Kibugu ,
James Ngugi Mwangi ,
Japhet Mutegi Kiragu ,
Margaret Wanjiku Muchiri ,
Kariuki Ndung'u  and
Raymond Ellie Mdachi
James Karuku Kibugu*
Affiliation:
Kenya Agricultural Research Institute (KARI), Trypanosomiasis Research Centre, PO Box 362-00902 Kikuyu, Kenya
James Ngugi Mwangi
Affiliation:
KARI, Social Economics and Biometrics Division, PO Box 57811-00200, Nairobi, Kenya
Japhet Mutegi Kiragu
Affiliation:
Kenya Agricultural Research Institute (KARI), Trypanosomiasis Research Centre, PO Box 362-00902 Kikuyu, Kenya
Margaret Wanjiku Muchiri
Affiliation:
Kenya Agricultural Research Institute (KARI), Trypanosomiasis Research Centre, PO Box 362-00902 Kikuyu, Kenya
Kariuki Ndung'u
Affiliation:
Kenya Agricultural Research Institute (KARI), Trypanosomiasis Research Centre, PO Box 362-00902 Kikuyu, Kenya
Raymond Ellie Mdachi
Affiliation:
Kenya Agricultural Research Institute (KARI), Trypanosomiasis Research Centre, PO Box 362-00902 Kikuyu, Kenya
*
*E-mail: [email protected]
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Abstract

Homidium bromide is a broad-spectrum anti-microbial trypanocide likely to be encountered as a violative residue in blood collected from abattoirs destined for feeding laboratory-reared tsetse colonies. We investigated its effects on longevity of laboratory-reared Glossina morsitans morsitans Westwood. Four steers were intra-muscularly administered with 1 mg homidium bromide/kg of body weight and blood was aseptically collected from them between 15 and 30 min post-administration. This blood was defibrinated, analysed for homidium levels, screened for bacterial contamination, frozen and warmed to 37°C before feeding to tsetse flies. Teneral male (100) and female (220) G. m. morsitans flies were fed on homidium-treated diet, and control flies (99 males and 187 females) on untreated blood diet and their survival monitored for 163 days. Homidium, at 266.15 ng/ml blood diet, significantly (P < 0.05) improved fly survival. We concluded that homidium bromide has a beneficial effect on tsetse, probably attributable to its antimicrobial activity against unfavourable microbes mediated by the drug, and could be used as a tsetse diet additive.

Keywords

Glossina morsitans morsitanshomidiumsurvival
Type
Short Communication
Information
International Journal of Tropical Insect Science , Volume 30 , Issue 3 , September 2010 , pp. 163 - 166
Copyright
Copyright © ICIPE 2010

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