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Improved artificial diet for mass rearing of the tobacco caterpillar, Spodoptera litura (Lepidoptera: Noctuidae)

Published online by Cambridge University Press:  28 February 2007

G.P. Gupta ,
S. Rani ,
A. Birah  and
M. Raghuraman
G.P. Gupta*
Affiliation:
Division of Entomology, Indian Agricultural Research Institute, New Delhi 110012, India
S. Rani
Affiliation:
Division of Entomology, Indian Agricultural Research Institute, New Delhi 110012, India
A. Birah
Affiliation:
Division of Entomology, Indian Agricultural Research Institute, New Delhi 110012, India
M. Raghuraman
Affiliation:
Division of Entomology, Indian Agricultural Research Institute, New Delhi 110012, India
*
*Email: [email protected]
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Abstract

An artificial diet for mass rearing of the tobacco caterpillar Spodoptera litura (Fabricius) from the neonate to adult stage was developed at 27±1°C, 65±5% RH and 16:8 h scoto/photo-phase regime. The diet ingredients consisted of wheat germ (26.0 g), kidney bean flour (51.3 g), chickpea flour (56.0 g), yeast powder (31.6 g), casein (15.2 g), l-ascorbic acid (3.2 g), cholesterol (0.5 g), two multivitamin multimineral capsules, one vitamin E capsule, ABDEC drops (2 ml), castor oil (1 ml), methyl-p-hydroxybenzoate (1.8 g), sorbic acid (1.3 g), streptomycin sulphate (0.25 g), formaldehyde solution (2 ml), agar–agar (16.4 g) and 820 ml distilled water. The new artificial diet successfully supported the growth and development for more than ten generations with enhanced reproductive potential. The mean biological parameters based on rearing of ten continuous generations showed higher pupation (89.2%), emergence (97.2%), survival (86.6%) and fecundity (2486.2 eggs) as compared to the most preferred natural food, castor leaf whose respective recorded values are 80, 75, 60% and 480 eggs. The cost of 1 l of the diet was approximately US$ 2.00 only, on which 200 neonate larvae can be reared. The artificial diet developed is suitable for mass rearing S. litura successfully throughout the year for laboratory and field experimentation as well as for commercial production of nuclear polyhedrosis viruses (Sl NPV).

Keywords

Spodoptera lituraartificial dietmass rearingcastor leaf
Type
Short Communication
Information
International Journal of Tropical Insect Science , Volume 25 , Issue 1 , March 2005 , pp. 55 - 58
Copyright
Copyright © ICIPE 2005

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References

Cohen, A. C. (2001) Formalizing insect rearing and artificial diet technology American Entomologist 47, 198–206.CrossRefGoogle Scholar
Finney, D. J. (1971) Probit Analysis. Cambridge University Press, London. 333 pp.Google Scholar
Gupta, G. P., Mahapatro, G. K., Ajanta, C. (1998) Partial larval rearing of Earias vittella (Fabricius) Insect Science and its Application 18, 371–375.Google Scholar
Janakiraman, S., Gupta, G. P. (2002) Effect of modified artificial diet and insecticidal proteins on growth and development of tobacco cutworm ( Spodoptera litura ) Indian Journal of Agricultural Sciences 72, 719–725.Google Scholar
Miller, T. A., Cooper, W. J., Highfill, J. W. (1982) Relationship between pupal size and egg production in reared female Antheraea polyphemus Annals of the Entomological Society of America 75, 107–108.CrossRefGoogle Scholar
Patel, R. C., Patel, J. C., Patel, J. K. (1973) Biology and mass breeding of the tobacco caterpillar, Spodoptera litura (F) Israel Journal of Entomology 8, 131–140.Google Scholar
Seth, R. K., Sharma, V. P. (2001) Inherited sterility by substerilizing radiation in Spodoptera litura (Lepidoptera: Noctuidae): Bioefficacy and potential for pest suppression. Florida Entomologist 84, 183–193.CrossRefGoogle Scholar
Yang, Ou S. C., Chu, Y. I. (1988) The comparison of the development of the tobacco cutworm, Spodoptera litura (F.) reared with natural and artificial diets Chinese Journal of Entomology 8, 143–150.Google Scholar