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Effect of pH, rearing temperature and sunlight on infectivity of Malaysian isolate of nucleopolyhedrovirus to larvae of Spodoptera litura (Lepidoptera: Noctuidae)

Published online by Cambridge University Press:  01 June 2007

A.S. Sajap ,
M.A. Bakir ,
H.A. Kadir  and
N.A. Samad
A.S. Sajap*
Affiliation:
Department of Forest Management, Universiti Putra Malaysia, 43400, UPM, Serdang, Selangor, Malaysia
M.A. Bakir
Affiliation:
Department of Forest Management, Universiti Putra Malaysia, 43400, UPM, Serdang, Selangor, Malaysia
H.A. Kadir
Affiliation:
Malaysian Agricultural Research Development Institute, PO Box 12341, Kuala Lumpur, Malaysia
N.A. Samad
Affiliation:
Department of Biochemistry, Universiti Putra Malaysia, 43400, UPM, Serdang, Selangor, Malaysia
*
*E-mail: [email protected]
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Abstract

The effects of pH, sunlight and rearing temperature on infectivity of nucleopolyhedrovirus (SpltNPV) were investigated against larvae of Spodoptera litura (Fabricius). The infectivity of virus increased with increased larval rearing temperature. The LT50 values were significantly reduced from 24 to 6 days when rearing temperature was increased from 20 to 35 °C. The pH of the substrate significantly affected the infectivity of the virus. The LT50 values increased to about fourfold when the pH of suspension was raised from 7 to 11. Infectivity of the virus was more adversely affected in alkaline than in acidic suspension. Sunlight also deleteriously affected infectivity of the virus. The LT50 values increased with the increase in the period of sunlight exposure. A complete viral inactivation occurred after 12 h of direct sunlight exposure.

Keywords

nucleopolyhedrovirusSpodoptera lituraMalaysia
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 27 , Issue 2 , June 2007 , pp. 108 - 113
Copyright
Copyright © ICIPE 2007

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References

Ali, A. and Young, S. Y. (1991) Influence of larval age and temperature on effectiveness of a nuclear polyhedrosis virus in soybean looper Pseudoplusia includens (Lepidoptera: Noctuidae) on soybean. Biological Control 1, 334–338.CrossRefGoogle Scholar
Behle, R. W., McGuire, M. R. and Tamez-Guerra, P. (2000) Effect of light energy on alkali-released virions from Anagrapha falcifera. Journal of Invertebrate Pathology 76, 120–126.CrossRefGoogle ScholarPubMed
Evans, H. and Shapiro, M. (1997) Viruses, pp. 17–53. In Biological Techniques: Manual of Techniques in Insect Pathology (Edited by Lacey, L. A.). Academic Press, London, UK.CrossRefGoogle Scholar
Finney, D. J. (1971) Probit Analysis. Cambridge University Press, Cambridge, UK.Google Scholar
Griego, V. M., Martignoni, M. E. and Claycomb, A. E. (1985) Inactivation of nuclear polyhedrosis virus (Baculovirus subgroup A) by monochromatic UV radiation. Applied and Environmental Microbiology 49, 709–710.CrossRefGoogle ScholarPubMed
Grzywacz, D., McKinley, D. J., Jones, K. A. and Moawad, G. (1997) Microbial contamination in Spodoptera littoralis nuclear polyhedrosis virus produced in insects in Egypt. Journal of Invertebrate Pathology 69, 151–156.CrossRefGoogle Scholar
Ignoffo, C. M. (1966) Effects of temperature on mortality of Heliothis zea larvae exposed to sublethal doses of a nuclear-polyhedrosis virus. Journal of Invertebrate Pathology 8, 290–292.CrossRefGoogle ScholarPubMed
Ignoffo, C. M. and Garcia, C. (1996) Simulated sunlight UV sensitivity of experimental dust formulations of the nuclear polyhedrosis virus of Helicoverpa/Heliothis. Journal of Invertebrate Pathology 67, 192–194.CrossRefGoogle Scholar
Jaques, R. P. (1970) Application of viruses to soil and foliage for control of the cabbage looper and imported cabbageworm. Journal of Invertebrate Pathology 15, 328–340.CrossRefGoogle Scholar
Jaques, R. P. (1985) Stability of insect viruses in the environment, pp. 285–360. In Viral Insecticides for Biological Control (Edited by Maramorosch, K. and Sherman, K. E.). Academic Press, New York.CrossRefGoogle Scholar
Johnson, D. W., Boucias, D. B., Barfield, C. C. and Allen, G. E. (1982) A temperature-dependent developmental model for a nucleopolyhedrovirus of the velvet green caterpillar, Anticarsia gemmatalis. Journal of Invertebrate Pathology 40, 292–298.CrossRefGoogle Scholar
Keating, S. T., Schultz, J. C. and Yendol, W. G. (1990) The effect of diet on gypsy moth (Lymantria dispar) larval midgut pH, and its relationship with larval susceptibility to a baculovirus. Journal of Invertebrate Pathology 56, 317–326.CrossRefGoogle Scholar
Knittel, M. D. and Fairbrother, A. (1987) Effects of temperature and pH on survival of free nuclear polyhedrosis virus of Autographa californica. Applied and Environmental Microbiology 53, 2771–2773.CrossRefGoogle ScholarPubMed
Lacey, A. L. and Brooks, W. M. (1997) Initial handling and diagnosis of diseased insects, pp. 1–16. In Biological Techniques: Manual of Techniques in Insect Pathology (Edited by Lacey, L. A.). Academic Press, London, UK.Google Scholar
McLeod, P. J., Yearin, W. C. and Young, Y. S. (1977) Inactivation of Baculovirus heliothis by ultraviolet irradiation, dew, and temperature. Journal of Invertebrate Pathology 30, 237–241.CrossRefGoogle Scholar
Monobrullah, M. D. and Nagata, M. (2001) Optical brighteners as ultraviolet protectants and as enhancers in pathogenicity of Spodoptera litura (Fabricius) (Lep., Noctuidae) nucleopolyhedrovirus). Journal of Applied Entomology 125, 377–382.CrossRefGoogle Scholar
Moscardi, F. and Sosa-Gomez, D. R. (1992) Uses of viruses against soybean caterpillar in Brazil, pp. 98–109. In Pest Management in Soybean (Edited by Copping, L. G., Green, M. B. and Ness, R. T.). Elsevier Applied Science, London, UK.CrossRefGoogle Scholar
Navon, A. (1985) Spodoptera littoralis, pp. 469–475. In Handbook of Insect Rearing (Edited by Singh, P. and Moore, R. F.). Vol. 2. Elsevier, Amsterdam, The Netherlands.Google Scholar
Pritchett, D. W., Young, S. Y. and Aaron, W. C. (1982) Dissolution of Autographa californica nuclear polyhedrosis virus polyhedra by the digestive fluid of Trichoplusia ni (Lepidoptera: Noctuidae) larvae. Journal of Invertebrate Pathology 39, 354–361.CrossRefGoogle Scholar
Ribeiro, H. C. T. and Pavan, O. H. O. (1994) Effect of temperature on the development of baculoviruses. Journal of Applied Entomology 118, 316–320.CrossRefGoogle Scholar
Rohrmann, G. F. (1992) Review: Baculovirus structural proteins. Journal of General Virology 73, 749–761.CrossRefGoogle Scholar
Sajap, A. S. (1995) Prospect of biological control of forest insects in Malaysia. Malaysian Forester 58, 51–56.Google Scholar
Sajap, A. S., Kotulai, J. R., Bakir, M. A., Lau, W. H., Kadir, H. A. and Samad, N. A. (2000) Pathogenicity and characteristics of Spodoptera litura nucleopolyhedrovirus from Peninsular Malaysia. Pertanika Journal of Tropical Agricultural Science 23, 23–28.Google Scholar
Sajap, A. S., Kotulai, J. R., Kadir, H. A. and Hussein, M. Y. (1999) Impact of prey infected by nuclear polyhedrosis virus on a predator, Sycanus leucomesus Walk. (Hemiptera: Reduviidae). Journal of Applied Entomology 123, 93–97.CrossRefGoogle Scholar
Shapiro, M. and Argauer, R. (1995) Effects of pH, temperature and ultraviolet radiation on the activity of an optical brightener as a viral enhancer for the gypsy moth (Lepidoptera: Lymantriidae) baculovirus. Journal of Economic Entomology 88, 1602–1606.CrossRefGoogle Scholar
Silva, M. T. B. and Moscardi, F. (2002) Field efficacy of the nucleopolyhedrovirus of Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae): Effect of formulations, water pH, volume and time of application, and type of spray nozzle. Neotropical Entomology 31, 75–83.CrossRefGoogle Scholar
Smirnoff, W. A. (1972) Effect of sunlight on the nuclear-polyhedrosis virus of Neodiprion swainei with measurement of the solar energy received. Journal of Invertebrate Pathology 19, 179–188.CrossRefGoogle Scholar
Van Beek, N. V., Hughes, P. R. and Wood, H. A. (2000) Effects of incubation temperature on the dose-survival time relationship of Trichoplusia ni larvae infected with Autographa californica nucleopolyhedrovirus. Journal of Invertebrate Pathology 76, 185–190.CrossRefGoogle ScholarPubMed