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Carriage of micro-organisms by domestic cockroaches and implications on food safety

Published online by Cambridge University Press:  01 September 2006

S. Mpuchane ,
J. Allotey ,
I. Matsheka ,
M. Simpanya ,
S. Coetzee ,
A. Jordaan ,
N. Mrema  and
B.A. Gashe
S. Mpuchane
Affiliation:
Department of Biological Sciences, PO Box UB 00704, University of Botswana, Gaborone, Botswana
J. Allotey*
Affiliation:
Department of Biological Sciences, PO Box UB 00704, University of Botswana, Gaborone, Botswana
I. Matsheka
Affiliation:
Department of Biological Sciences, PO Box UB 00704, University of Botswana, Gaborone, Botswana
M. Simpanya
Affiliation:
Department of Biological Sciences, PO Box UB 00704, University of Botswana, Gaborone, Botswana
S. Coetzee
Affiliation:
Electron Microscope Unit, Faculty of Science, PO Box UB 00704, University of Botswana, Gaborone, Botswana
A. Jordaan
Affiliation:
Electron Microscope Unit, Faculty of Science, PO Box UB 00704, University of Botswana, Gaborone, Botswana
N. Mrema
Affiliation:
Department of Biological Sciences, PO Box UB 00704, University of Botswana, Gaborone, Botswana
B.A. Gashe
Affiliation:
Department of Biological Sciences, PO Box UB 00704, University of Botswana, Gaborone, Botswana
*
*E-mail: [email protected]
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Abstract

Domestic cockroaches Blattella germanica were trapped from various homes in Gaborone, Botswana using ‘Dyroach’ traps. Isolations of bacteria, yeasts and moulds were done on external body parts and on the faecal pellets using various selective media. Body parts of cockroaches were also fixed with OsO4 vapour for 24 h, sputter coated with gold under special conditions and examined in a Phillips (1) XL30 ESEM in low vacuum mode. A wide spectrum of bacteria including common food spoilage and pathogenic organisms, such as Bacillus spp., Staphylococcus spp., Escherichia coliPseudomonas spp., Enterobacter spp., Klebsiella spp., Erwinia spp. Salmonella sp., Shigella sp. and Serratia spp. were isolated. Yeasts commonly associated with food spoilage, such as Pichia sp., Candida sp. and Torulopsis spp. were found on many cockroaches. In addition, various groups of moulds, some associated with food spoilage and others known to produce mycotoxins, such as Aspergillus flavusAspergillus fumigatus and Aspergillus parasiticus were isolated. Antimicrobial sensitivities of bacterial isolates revealed resistance patterns to various agents. Since cockroaches are prevalent in homes, particularly in food preparation areas and since their body parts and faecal pellets have been found in food storage areas, concern about their impact on food quality and safety is raised and possible control measures including education of communities on proper sanitation are suggested.

Résumé

Des blattes domestiques Blattella germanica ont été piégées dans plusieurs maisons à Gaborone, au Botswana à l'aide de pièges ‘Dyroach’. On a réalisé des isolements de bactéries, de levures et de moisissures à partir de plusieurs morceaux d'exuvie et des fèces en utilisant différents milieux de culture sélectifs. Des morceaux d'exuvie ont également été fixés avec des vapeurs d'OsO4 pendant 24 h, recouverts d'une couche d'or et examinés en microscopie électronique à balayage sous vide à l'aide d'un microscope Phillips (1) XL30 ESEM. On a réussi à isoler un vaste spectre de bactéries comprenant des organismes pathogènes et présents dans les déchets de nourriture tels que Bacillus spp., Staphylococcus spp., Escherichia coliPseudomonas spp., Enterobacter spp., Klebsiella spp., Erwinia spp., Salmonella sp., Shigella sp. et Serratia spp. Des levures fréquemment associées aux déchets de nourriture, telles que Pichia sp., Candida sp. et Torulopsis spp. ont été trouvées sur plusieurs blattes. De plus, plusieurs groupes de moisissures, certains associés aux déchets de nourriture, d'autres connus pour produire des mycotoxines tels qu'Aspergillus flavusAspergillus fumigatus et Aspergillus parasiticus ont été isolés. Des tests de sensibilité aux antibiotiques appliqués à plusieurs isolats bactériens ont permis la mise en évidence de forme de résistance à différents antibiotiques. Dans la mesure où les blattes sont très communes dans les habitations, en particulier sur les lieux de préparation des aliments et où leurs exuvies et leurs fèces ont été trouvés dans les lieux de stockage de la nourriture, on a pu montrer l'importance de leur impact sur la qualité et l'innocuité de la nourriture, imaginer des mesures de prévention et suggérer l'éducation des communautés sur des mesures sanitaires adaptées.

Keywords

cockroachespathogenic micro-organismsantibiotic resistancebacteriafungiblattesmicroorganismes pathogènesrésistance antibiotique
Type
Research Article
Information
International Journal of Tropical Insect Science , Volume 26 , Issue 3 , September 2006 , pp. 166 - 175
Copyright
Copyright © ICIPE 2006

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References

Abdel Aal, A. N. I. and Mazen, N. A. M. (1995) Role of (adults and nymphs) German cockroaches in transmission of toxigenic fungi and some nematode parasites in man's food. Australian Veterinary Journal 32, 143.Google Scholar
Bauer, A. W., Kirby, W. M. M., Sherris, J. C. and Turk, M. (1966) Antibiotic susceptibility testing by a standard single disc diffusion method. American Journal of Clinical Pathology 45, 493–496.Google Scholar
Bennet, G. (1993) Cockroaches as carriers of bacteria. Lancet (British Edition) 341, 732.Google Scholar
Boase, C. J. (1999) Trends in urban refuse disposal: A pest's perspective, pp. 83–98. In Proceedings of the 3rd International Conference on Urban Pests, 19–22 July 1999, Czech University of Agriculture, Prague, Czech Republic (Edited by Robinson, Wm. H., Rettich, F. and Rambo, G. W.). Grafické závody Hronov, Czech Republic.Google Scholar
Branscone, D. (2002) Disease-transmitting pests. Pest Control Technology 30, 82–84.Google Scholar
Brossut, R. (1983) Allomonal secretions in cockroaches. Journal of Chemical Ecology 9, 143–158.Google Scholar
Cloarec, A., Rivault, G., Fontaine, F. and LeGuyader, A. (1992) Cockroaches as carriers of bacteria in multifamily dwellings. Epidemiology of Infection 109, 483–490.Google Scholar
Cotton, M. F., Wasserman, E., Pieper, C. H., Theron, D. C., Tubbergh van, D., Campbell, G., Fang, F. C. and Barnes, G. (2000) Invasive disease due to extended spectrum beta-lactamase-producing Klebsiella pneumoniae in a neonatal unit: The possible role of cockroaches. The Journal of Hospital Infection 44, 13–17.Google Scholar
De Jonge, J. T. (1993) Cockroach control in the Netherlands, pp. 305–307. In Proceedings of the 1st International Conference on Insect Pests in the Urban Environment, 30 June – 3 July 1993, St John's College, Cambridge, UK (Edited by Wildey, K. B. and Robinson, W. H.). BPCC Wheatons Ltd, Exeter, UK.Google Scholar
Devi, S. J. N. and Murray, C. J. (1991) Cockroaches (Blatta and Periplaneta spp.) as reservoirs of drug resistant Salmonella. Epidemiology and Infection 107, 35–361.Google Scholar
Fotedar, R. and Banerjee, U. (1992) Nosocomial fungal infections: Study of the possible role of cockroaches (Blattella germanica) as vectors. Acta Tropica 50, 339–343.Google Scholar
Glazer, A. N. and Nikaido, H. (1995) Microbial Biotechnology: Fundamentals of Applied Microbiology. W.H. Freeman and Company, New York.Google Scholar
Gliniewicz, A., Czajka, E., Laudy, A. E., Kochman, M., Crzegorzak, K., Ziolkowska, K., Sawicka, B., Stypulkowska-Misiurewicz, H. and Hopskins, J. A. (2003) German cockroaches (Blattella germanica L.) as a potential source of pathogens causing nosocomial infections. Indoor Built Environment 1012, 55–60.Google Scholar
Gratz, N. G. (1999) Urbanization, arthropod and rodent pests and human health, pp. 51–58. In Proceedings of the 3rd International Conference on Urban Pests, 19–22 July 1999. Czech University of Agriculture, Prague, Czech Republic (Edited by Robinson, Wm. H., Rettich, F. and Rambo, G. W.). Grafické závody Hronov, Czech Republic.Google Scholar
Jay, J. M. (1992) Modern Food Microbiology, 4th edn. Chapman Hall, London.Google Scholar
Kitae, Kim, JinHwa, Jeon and DongKyu, Lee (1995) Various pathogenic bacteria on German cockroaches (Blattellidae: Blattaria) collected from general hospitals. Korean Journal of Entomology 25, 85–88.Google Scholar
King, A. D., Hoking, A. D. and Pitt, J. I. (1979) Dichloran rose Bengal medium for the enumeration of molds from foods. Applied and Environmental Microbiology 37, 959–964.Google Scholar
Kopanic, R. J. Jr, Sheldon, B. W. and Wright, C. G. (1994) Cockroaches as vectors of salmonella: Laboratory and field trials. Journal of Food Protection 57, 125–132.Google Scholar
Lemos, A. A., Lemos, J. A., Prado, M. A., Pimenta, F. C., Gir, E., Silva, H. M. and Silva, M. R. R. (2006) Cockroaches as carriers of fungi of medical importance. Mycoses 49, 23–25.Google Scholar
Marty, A. M. (1998) Cockroaches can vector human diseases. International Journal of Dermatology 37, 639–640.Google Scholar
Mpuchane, S. and Gashe, B. A. (1996) Prevalence of coliforms in traditionally dried leafy vegetables sold in open markets and food stores in Gaborone, Botswana. Journal of Food Protection 59, 28–30.Google Scholar
Mpuchane, S., Allotey, J., Gashe, B. A., Matsheka, M. I., Coetzee, A., Jordaan, A. and Oteng, M. (2005) Association between German cockroaches (Blattella germanica) and street food vending: Implications for food safety in Botswana, pp. 123–130. In Proceedings of the 5th International Conference on Insect Pests in Urban Environment, 19 – 22 July 2005 Suntech, Singapore (Edited by Chang, Lee and Robinson, W. H.). Perniagaan Ph'ng @ P. & Y Design Network, Malaysia.Google Scholar
Mpuchane, S., Gashe, B. A., Allotey, J., Siame, B., Teferra, G. and Ditlhogo, M. (2000) Quality deterioration of phane, the edible caterpillar of an emperor moth Imbrasia belina. Food Control 11, 453–458.Google Scholar
Oteng M. (2003) Insects as vectors of pathogenic microorganisms in street vended foods. BSc project. Department of Biological Sciences, University of Botswana. 53 pp.Google Scholar
Paul, S., Khan, A. M., Baqui, M. A. and Muhibullah, M. (1992) Evaluation of the common cockroach Periplaneta americana (L) as carrier of medically important bacteria. Journal of Communicable Diseases 24, 206–210.Google Scholar
Pitt, J. I. (1979) The Genus Penicillium and Its Teleomorphic States Eupenicillium and Talaromyces. Academic Press, London.Google Scholar
Pitt, J. I. and Hocking, A. D. (1985) Fungi and Spoilage. Academic Press, London.Google Scholar
Pitt, J. I. and Hocking, A. D. (1994) Modern methods for detecting and enumerating food-borne fungi, pp. 233–254. In Rapid Analysis Techniques in Food Microbiology (Edited by Patel, P. D.). Blackie Academic and Professional, London.Google Scholar
Prado, M. A., Pimenta, F. C., Hayashid, M., Souza, P. R., Pereira, M. S. and Gir, E. (2002) Enterobacteria isolated from cockroaches (Periplaneta americana) captured in a Brazilian hospital. Pan American Journal of Public Health 11, 93–98.Google Scholar
Rivault, G., Cloarec, A. and LeGuyader, A. (1993a) Bacterial load of cockroaches in relation to urban environment. Epidemiology and Infection 110, 317–325.Google Scholar
Rivault, G., Cloarec, A. and LeGuyader, A. (1993b) Bacterial contamination of food by cockroaches. Journal of Environmental Health 55, 21–22.Google Scholar
Rose, L. M. and Eisner, T. (1962) Chemical defenses of arthropods. Annual Review of Entomology 7, 107–136.Google Scholar
Samson, R. A. and van Reenen-Hoekstra, E. S. (1999) Introduction To Some Food-Borne Fungi, 3rd edn. Centralbureau voor Schimmelcultures, Bacern, Netherlands.Google Scholar
Siame, B. A., Mpuchane, S. F., Gashe, B. A., Allotey, J. and Teferra, G. (1998) Occurrence of aflatoxins, fumonisin B1 and zearalenone in foods and feeds in Botswana. Journal of Food Protection 61, 1670–1673.Google Scholar
Sramova, H., Daniel, M., Absolonova, V., Dedicova, D., Jedlickova, Z., Urotova, H., Petras, P. and Subertova, V. (1992) Epidemiological role of arthropods detectable in health facilities. Journal of Hospital Infection 20, 281–292.CrossRefGoogle ScholarPubMed
Vythilingam, I., Jeffery, J., Oothuman, P., Abdul Razak, A. and Sulaimman, A. (1997) Cockroaches from urban human dwellings: Isolation of bacterial pathogens and control. Southeast Asian Journal of Tropical Medicine and Public Health 28, 218–222.Google Scholar