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Microwave dielectrometry as a tool for the characterization of blood cell membrane activity for in vitro diagnostics

Published online by Cambridge University Press:  02 May 2017

Kateryna Arkhypova*
Affiliation:
O.Ya. Institute for Radiophysics and Electronics NAS of Ukraine, 12 Proskura st., Kharkiv, 61085, Ukraine. Phone: +38 0730 313 918 Kharkiv Medical Academy of Post-Graduate Education, Department of Rehabilitation, Sport Medicine and Exercise Therapy, 58 Amosova st., Kharkiv, 61176, Ukraine
Pavlo Krasov
Affiliation:
O.Ya. Institute for Radiophysics and Electronics NAS of Ukraine, 12 Proskura st., Kharkiv, 61085, Ukraine. Phone: +38 0730 313 918
Anatolii Fisun
Affiliation:
O.Ya. Institute for Radiophysics and Electronics NAS of Ukraine, 12 Proskura st., Kharkiv, 61085, Ukraine. Phone: +38 0730 313 918
Andriy Nosatov
Affiliation:
Department of Neurology, City Clinical Hospital No 7, 266 Saltivs'ke Hwy, Kharkiv, Ukraine
Volodymyr Lychko
Affiliation:
Department of Neurology, Medical Institute of Sumy State University, 38, Metalurgiv st., 40004, Sumy, Ukraine
Volodymyr Malakhov
Affiliation:
Kharkiv Medical Academy of Post-Graduate Education, Department of Rehabilitation, Sport Medicine and Exercise Therapy, 58 Amosova st., Kharkiv, 61176, Ukraine
*
Corresponding author: K. Arkhypova Email: [email protected]

Abstract

This paper deals with the concept of microliter sensing of human blood samples by waveguide dielectrometry at microwave frequency (39.5 GHz). The methods, research protocol, and basic results derived from the permittivity measurements are presented. Here we summarize the results of our 5-year research on developing a multidisciplinary approach to the characterization of erythrocytes dielectric response, in parallel with biochemical studies of their receptor-membrane activity, within the scope of a case–control study for in vitro diagnostics of acute and chronic neurological conditions.

Type
Research Papers
Copyright
Copyright © Cambridge University Press and the European Microwave Association 2017 

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References

REFERENCES

[1] Grenier, K. et al. : Recent advances in microwave-based dielectric spectroscopy at the cellular level for cancer investigations. IEEE Microw. Theory Tech., 61 (5) (2013), 20232030.CrossRefGoogle Scholar
[2] Polevaya, Y.; Ermolina, I.; Schlesinger, M.; Ginzburg, B.-Z.; Feldman, Y.: Time domain dielectric spectroscopy study of human cells II. Normal and malignant white blood cells. Biochim. Biophys. Acta, 1419 (1999), 257271.Google Scholar
[3] Irimajiri, A.; Ando, M.; Matsuoka, R.; Ichinowatari, T.; Takeuchi, S.: Dielectric monitoring of rouleaux formation in human whole blood: a feasibility study. Biochim. Biophys. Acta, 1290 (1996), 207209.Google Scholar
[4] Hayashi, Y.; Oshige, I.; Katsumoto, Y.; Omori, S.; Yasuda, A.; Asami, K.: Dielectric inspection of erythrocyte morphology. Phys. Med. Biol., 53 (2008), 25532564.Google Scholar
[5] Sainani, K.L.: Dealing with non-normal data. Phys. Med. Rehabil., 4 (2012), 10011005.Google Scholar
[6] Kitchen, Ch. M. R.: Nonparametric versus parametric tests of location in biomedical research. Am. J. Ophthalmol., 147 (4) (2009), 571572.Google Scholar
[7] Schwan, H. P.: Electrical properties of blood and its constituents: alternating current spectroscopy. Blut, 46 (1983), 185197.Google Scholar
[8] Shchegoleva, T. Yu.: The Investigation of Biological Objects in the Millimeter-Wave Range, Naukova Dumka, Kiev, 1996 (in Russian).Google Scholar
[9] Krasov, P.S.; Arkhypova, K.A.: Instrument for measuring the complex permittivity of biological objects. Telecommun. Radio Eng., 68 (8) (2009), 727733.Google Scholar
[10] Krasov, P. S.: Sensitization of waveguide measuring cuvette for biological objects permittivity investigation. Telecommun. Radio Eng., 70 (6) (2011), 491496.Google Scholar
[11] Sirenko, Y. K.; Ström, S.; Yashina, N. P.: Modeling and Analysis of Transient Processes in Open Resonant Structures: New Methods and Techniques, Springer, New York, 2007.Google Scholar
[12] Sato, T.; Buchner, R.: Dielectric relaxation processes in ethanol/water mixtures. J. Phys. Chem. A, 108 (23) (2004), 50075015.Google Scholar
[13] Krasov, P.S.; Arkhypova, K.A.: Calibration of microwave waveguide sensor for biomedical applications, in The IEEE Int. Conf. Mathematical Methods in Electromagnetic Theory, Lviv, Ukraine, 2016, 287290.Google Scholar
[14] Stryuk, R. I.; Dlusskaya, I. G.: Adrenoreactivity and Cardiovascular System, Meditsina, Moskow, 2003 (in Russian).Google Scholar
[15] Arkhypova, K.A.: Monitoring the functional properties of human red blood cells by means of waveguide microwave single-frequency dielectrometry method. Telecommun. Radio Eng., 70 (6) (2011), 547552.Google Scholar
[16] Fellows, I.: Deducer: a data analysis GUI for R. J. Stat. Softw., 49 (2012), 115. http://www.jstatsoft.org/v49/i08/.Google Scholar
[17] Arkhypova, K.A.; Krasov, P.S.; Fisun, A.I.: Application of modified waveguide sensor for dielectric study of red blood cells of patients with discirculatory encephalopathy before and after the therapy. Biofizychnyi visnyk, 27 (2) (2011), 93102 (in Russian).Google Scholar