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Dosimetry of an in vitro exposure system for fluorescence measurements during 2.45 GHz microwave exposure

Published online by Cambridge University Press:  25 November 2010

Mohamad Kenaan ,
Mihaela G. Moisescu ,
Tudor Savopol ,
Diana Martin ,
Delia Arnaud-Cormos  and
Philippe Leveque
Mohamad Kenaan
Affiliation:
XLIM Research Institute, University of Limoges/CNRS, 123 avenue Albert Thomas, 87060 Limoges Cedex, France. Phone: +33 555 45 75 21.
Mihaela G. Moisescu
Affiliation:
Department of Biophysics and Cell Biotechnologies, Carol Davila Medical University, 8 Eroilor Sanitari Blv., P.O. Box 35-43, Bucharest, Romania.
Tudor Savopol
Affiliation:
Department of Biophysics and Cell Biotechnologies, Carol Davila Medical University, 8 Eroilor Sanitari Blv., P.O. Box 35-43, Bucharest, Romania.
Diana Martin
Affiliation:
National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, 077125 Magurele, Romania.
Delia Arnaud-Cormos*
Affiliation:
XLIM Research Institute, University of Limoges/CNRS, 123 avenue Albert Thomas, 87060 Limoges Cedex, France. Phone: +33 555 45 75 21.
Philippe Leveque
Affiliation:
XLIM Research Institute, University of Limoges/CNRS, 123 avenue Albert Thomas, 87060 Limoges Cedex, France. Phone: +33 555 45 75 21.
*
Corresponding author: D. Arnaud-Cormos Email: [email protected]
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Abstract

An in vitro system for 2.45 GHz microwave (MW) exposure with real-time fluorescence measurements is proposed. This system is specifically designed for the measurement of those biophysical parameters of living cells or membrane models which can be quantified by spectrofluorometric methods (e.g. membrane generalized polarization (GP), membrane fluidity, membrane potential, etc.). The novelty of the system consists in the possibility to perform fluorescence measurements on the biological samples simultaneously with their exposure to MW. The MW applicator is an open ended coaxial antenna which is dipped into a cuvette. The distribution of electromagnetic field and specific absorption rate (SAR) in the cuvette are provided from a rigorous electromagnetic numerical analysis performed with a finite difference-time domain (FDTD) based tool. With this system, fluorescence measurements were used to calculate the membrane GP values of giant unilamellar vesicle suspensions that were acquired during exposure to a 1.2 W incident power. For this power, the SAR distribution and mean SAR value for the whole volume were calculated based on temperature measurements made at different positions inside the cuvette.

Keywords

Microwave exposureFDTDGeneralized polarizationFluorescence measurements
Type
Original Article
Information
International Journal of Microwave and Wireless Technologies , Volume 3 , Issue 1 , February 2011 , pp. 81 - 86
Copyright
Copyright © Cambridge University Press and the European Microwave Association 2010

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