Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-25T08:06:02.013Z Has data issue: false hasContentIssue false

Calbindin-D28k immunoreactivity in the mice thoracic spinal cord after space flight

Published online by Cambridge University Press:  22 May 2015

Valentina V. Porseva
Affiliation:
Department of Human Anatomy, Yaroslavl State Medical University, Yaroslavl, Russia
Valentin V. Shilkin
Affiliation:
Department of Human Anatomy, Yaroslavl State Medical University, Yaroslavl, Russia
Igor B. Krasnov
Affiliation:
Laboratory of Gravitational Biology, Institute of Biomedical Problems of Russian Academy of Sciences, Moscow, Russia
Petr M. Masliukov*
Affiliation:
Department of Normal Physiology, Yaroslavl State Medical University, Yaroslavl, Russia

Abstract

The aim of the work was to analyse changes in the location and morphological characteristics of calbindin (CB)-immunoreactive (IR) neurons of the thoracic spinal cord of C57BL/6N male mice after completion of a 30-day space flight on board the BION-M1 biosatellite (Russia, 2013). Space flight induced multidirectional changes of the number and morphological parameters of CB-positive neurons. The number of IR neurons increased in laminae I (from 10 to 17 neurons per section), II (from 42 to 67 cells per section) and IX (from two neurons per segment to two neurons per section), but CB disappeared in neurons of lamina VIII. Weightlessness did not affect the number of CB-IR neurons in laminae III–V and VII, including preganglionic sympathetic neurons. The cross-sectional area of CB-IR neurons decreased in lamina II and VII (group of partition cells) and increased in laminae III–V and IX. After a space flight, few very large neurons with long dendrites appeared in lamina IV. The results obtained give evidence about substantial changes in the calcium buffer system and imbalance of different groups of CB-IR neurons due to reduction of afferent information under microgravity.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Airaksinen, M.S., Thoenen, H. & Meyer, M. (1997). Eur. J. Neurosci. 9, 120127.CrossRefGoogle Scholar
Alvarez, F.J., Benito-Gonzalez, A. & Siembab, V.C. (2013). Ann. N. Y. Acad. Sci. 1279, 2231.Google Scholar
Andreev-Andrievskii, A.A., Shenkman, B.S., Popova, A.S., Dolgov, O.N., Anokhin, K.V., Soldatov, P.E., Vinogradova, O.L., Ilyin, E.A. & Sychev, V.N. (2014). Aerosp. Environ. Med. 48, 1427.Google Scholar
Baevsky, R.M., Baranov, V.M., Funtova, I.I., Diedrich, A., Pashenko, A.V., Chernikova, A.G., Drescher, J., Jordan, J. & Tank, J. (2007). Appl. Physiol. 103, 156161.CrossRefGoogle Scholar
Baimbridge, K.G., Celio, M.R. & Rogers, J.H. (1992). Trends Neurosci. 15, 303308.CrossRefGoogle Scholar
Barber, R.P., Phelps, P.E., Houser, C.R., Crawford, G.D., Salvaterra, P.M. & Vaughn, J.E. (1984). Comp. Neurol. 229, 329346.CrossRefGoogle Scholar
Bertrand, S.S. & Cazalets, J.R. (2011). Front. Neural Circuits 5, 115.CrossRefGoogle Scholar
Carr, P.A., Alvarez, F.J., Leman, E.A. & Fyffe, R.E. (1998). NeuroReport 9, 26572661.CrossRefGoogle Scholar
Clarke, H.A., Dekaban, G.A. & Weaver, L.C. (1998). Neuroscience 85, 863872.Google Scholar
Concu, А. (1989). Eur. J. Appl. Physiol. 59, 1015.CrossRefGoogle Scholar
Craig, A.D., Zhang, E.T. & Blomqvist, A. (2002). Pain 97, 105115.Google Scholar
Deuchars, S.A., Milligan, C.J., Stornetta, R.L. & Deuchars, J. (2005). Neuroscience 25, 10631070.Google Scholar
Eckberg, D.L. & Neurolab Autonomic Nervous System Team (2003). Acta Physiol. Scand. 177, 299311.Google Scholar
Fahandejsaadi, A., Leung, E., Rahaii, R., Bu, J. & Geula, C. (2004). NeuroReport 15, 443448.Google Scholar
Gorbunova, A.V. & Portugalov, V.V. (1976). Aviat. Space Environ. Med. 47, 708710.Google Scholar
Grigoriev, A.I., Koslovakaya, I.B. & Shenkman, B.S. (2004). Ross. Fiziol. J. Im. I.M. Sechenova 90, 508521.Google Scholar
Iacopino, A., Christakos, S., German, D., Sonsalla, P.K. & Altar, C.A. (1992). Brain Res. Mol. Brain Res. 13, 251261.CrossRefGoogle Scholar
Ishihara, A., Yamashiro, J., Matsumoto, A., Higashibata, A., Ishioka, N., Shimazu, T. & Ohira, Y. (2006). Neurochem. Res. 31, 411415.Google Scholar
Islamov, R.R., Mishagina, E.A., Tyapkina, O.V., Shajmardanova, G.F., Eremeev, A.A., Kozlovskaya, I.B., Nikolskij, E.E. & Grigorjev, A.I. (2011). Acta Astron. 68, 14691477.CrossRefGoogle Scholar
Krasnov, I.B. (1994). Adv. Space Biol. Med. 4, 85110.CrossRefGoogle Scholar
Levine, A.J., Hinckley, C.A., Hilde, K.L., Driscoll, S.P., Poon, T.H., Montgomery, J.M. & Pfaff, S.L. (2014). Nat. Neurosci. 17, 586593.Google Scholar
Li, X. & Clark, J.D. (2001). Neuroscience 105, 949956.Google Scholar
Li, Y.N., Sakamoto, H., Kawate, T., Cheng, C.X., Li, Y.C., Shimada, O. & Atsumi, S. (2005). Arch. Histol. Cytol. 68, 5770.CrossRefGoogle Scholar
Masliukov, P.M., Korobkin, A.A., Nozdrachev, A.D. & Timmermans, J.P. (2012). Auton. Neurosci. 167, 2733.CrossRefGoogle Scholar
McKay, S.E. & Oppenheim, R.W. (1991). J. Neurobiol. 22, 721733.Google Scholar
Molander, C. & Grant, G. (1995). Spinal cord cytoarchitecture. In The Nervous System, ed. Paxinos, G.San Diego: Academic Press, 3944.Google Scholar
Monroy-Gómez, J. & Torres-Fernández, O. (2013). Biomédica 33, 564573.Google Scholar
Morona, R., Lopez, J.M., Dominguez, L. & Gonzalez, A. (2007). Microsc. Res. Tech. 70, 101118.Google Scholar
Porseva, V.V., Shilkin, V.V., Korzina, M.B., Smirnova, V.P. & Maslyukov, P.M. (2013). Neurosci. Behav. Physiol. 43, 602606.Google Scholar
Ren, J.C., Fan, X.L., Song, X.A., Zhao, X.H., Chen, M.X. & Shi, L. (2012). Muscle Nerve 45, 6569.CrossRefGoogle Scholar
Renshaw, B. (1941). J. Neurophysiol. 4, 167183.Google Scholar
Rexed, B. (1952). Comp. Neurol. 96, 415496.CrossRefGoogle Scholar
Rosenberg, S.S. & Spitzer, N.C. (2011). Calcium signaling in neuronal development. Cold Spring Harb. Perspect. Biol. 3(10), a004259. doi: 10.1101/cshperspect.Google Scholar
Sanna, P.P., Celio, M.R., Bloom, F.E. & Rende, M. (1993). Proc. Natl. Acad. Sci. U.S.A. 90, 30483052.Google Scholar
Sato, T., Miyoshi, T., Nakazawa, K., Yano, H. & Takeoka, H. (2001). Gravit. Physiol. 8, 9799.Google Scholar
Schoenen, J. (1982). Neuroscience 7, 20572087.Google Scholar
Schwaller, B. (2012). Biochim. Biophys. Acta 1820, 12941303.CrossRefGoogle Scholar
Segizbaeva, M.O., Pogodin, M.A., Lavrov, I.N., Balykin, M.V. & Alexandrov, N.P. (2011). Hum. Physiol. 37, 52591.CrossRefGoogle Scholar
Sidman, R.L., Angevine, J.B. & Pierce, E.T. (1971). Atlas of the Mouse Brain and Spinal Cord. Harvard University Press, Cambridge, MA.Google Scholar
Sojka, D., Zacharova, G., Spicarova, D. & Palecek, J. (2010). Physiol. Res. 59, 10111017.Google Scholar
Sonetti, D.A., Wetter, T.J., Pegelow, D.F. & Dempsey, J.A. (2001). Respir. Physiol. 127, 185199.CrossRefGoogle Scholar
Stepien, A.E., Tripodi, М. & Arber, S. (2010). Neuron 68, 456472.Google Scholar
Tarabal, O., Caraballo-Miralles, V., Cardona-Rossinyol, A., Correa, F.J., Olmos, G., Lladó, J., Esquerda, J.E. & Calderó, J. (2014). J. Neuropathol. Exp. Neurol. 73, 519535.CrossRefGoogle Scholar
Thirumalai, V., Behrend, R.M., Birineni, S., Liu, W., Blivis, D. & O'Donovan, M.J. (2013). Neurophysiology 109, 702710.Google Scholar
Venturoli, D., Semino, P., Negrini, D. & Miserocchi, G. (1998). Acta Astronaut. 42, 185204.Google Scholar
Willis, W.D. & Coggeshall, R.E. (1991). Sensory Mechanisms of the Spinal Cord. Plenum Press, New York.Google Scholar
Xu, J.H., Yang, Z.B., Wang, H. & Tang, F.R. (2014). Neurosci. Lett. 561, 8085.Google Scholar
Yin, Q.W., Johnson, J., Prevette, D. & Oppenheim, R.W. (1994). J. Neurosci. 14, 76297640.Google Scholar