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A wide variety of putative extremophiles and large beta-diversity at the Mars Desert Research Station (Utah)

Published online by Cambridge University Press:  04 February 2011

Susana O.L. Direito ,
Pascale Ehrenfreund ,
Andries Marees ,
Martijn Staats ,
Bernard Foing  and
Wilfred F.M. Röling
Susana O.L. Direito
Affiliation:
Molecular Cell Physiology, Faculty of Earth and Life Sciences, VU University Amsterdam, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands
Pascale Ehrenfreund
Affiliation:
Leiden Institute of Chemistry, P O Box 9502, 2300 RA Leiden, The Netherlands Space Policy Institute, Elliott School of International Affairs, Washington, DC 20052, USA
Andries Marees
Affiliation:
Molecular Cell Physiology, Faculty of Earth and Life Sciences, VU University Amsterdam, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands
Martijn Staats
Affiliation:
Molecular Cell Physiology, Faculty of Earth and Life Sciences, VU University Amsterdam, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands
Bernard Foing
Affiliation:
European Space Agency, ESA ESTEC/SRE-S Postbus 299, 2200 AG Noordwijk, The Netherlands Petrology, Faculty of Earth and Life Sciences, VU University Amsterdam, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands
Wilfred F.M. Röling*
Affiliation:
Molecular Cell Physiology, Faculty of Earth and Life Sciences, VU University Amsterdam, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands
*
e-mail: [email protected]
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Abstract

Humankind's innate curiosity makes us wonder whether life is or was present on other planetary bodies such as Mars. The EuroGeoMars 2009 campaign was organized at the Mars Desert Research Station (MDRS) to perform multidisciplinary astrobiology research. MDRS in southeast Utah is situated in a cold arid desert with mineralogy and erosion processes comparable to those on Mars. Insight into the microbial community composition of this terrestrial Mars analogue provides essential information for the search for life on Mars: including sampling and life detection methodology optimization and what kind of organisms to expect. Soil samples were collected from different locations. Culture-independent molecular analyses directed at ribosomal RNA genes revealed the presence of all three domains of life (Archaea, Bacteria and Eukarya), but these were not detected in all samples. Spiking experiments revealed that this appears to relate to low DNA recovery, due to adsorption or degradation. Bacteria were most frequently detected and showed high alpha- and beta-diversity. Members of the Actinobacteria, Proteobacteria, Bacteroidetes and Gemmatimonadetes phyla were found in the majority of samples. Archaea alpha- and beta-diversity was very low. For Eukarya, a diverse range of organisms was identified, such as fungi, green algae and several phyla of Protozoa. Phylogenetic analysis revealed an extraordinary variety of putative extremophiles, mainly Bacteria but also Archaea and Eukarya. These comprised radioresistant, endolithic, chasmolithic, xerophilic, hypolithic, thermophilic, thermoacidophilic, psychrophilic, halophilic, haloalkaliphilic and alkaliphilic micro-organisms. Overall, our data revealed large difference in occurrence and diversity over short distances, indicating the need for high-sampling frequency at similar sites. DNA extraction methods need to be optimized to improve extraction efficiencies.

Keywords

Mars analogueextremophilesbeta-diversitydesertDNA recovery
Type
Research Article
Information
International Journal of Astrobiology , Volume 10 , Special Issue 3: Special issue: Astrobiology field research in Moon/Mars analogue environments , July 2011 , pp. 191 - 207
Copyright
Copyright © Cambridge University Press 2011

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