Neither a consensus statement nor a formal set of step-by-step guidelines for reprocessing rigid laryngoscopes have been published or endorsed by professional organizations. Several published guidelines, standards, and clinical reports were reviewed to evaluate the risk of nosocomial infection associated with the use of rigid laryngoscopes, to determine their minimum reprocessing requirements. This review found that the recommendations of some guidelines and standards for reprocessing rigid laryngoscopes are incomplete, inadequate, and inconsistent with one another, and that current practices for reprocessing rigid laryngoscopes are reported to be inadequate and lack standardization. It is recommended that a consensus statement be developed that standardizes the reprocessing of rigid laryngoscopes and requires cleaning followed by high-level disinfection (or sterilization) and drying of the rigid laryngoscope's blade and handle to prevent nosocomial infection, regardless of whether a protective barrier or sheath is used during the procedure.

Our goal was to assess the knowledge, attitudes, and practices regarding infection control and standard precautions among a group of nursing and midwifery instructors and students in Iran. A survey questionnaire was completed by 273 nursing and midwifery instructors and students at Shiraz University Medical Sciences during the period from May to November 2002. Two hundred thirty-one (90.9%) of the participants reported that they needed additional infection control education, especially on standard isolation precautions. There was a linear positive correlation between knowledge, practice, and attitude scores for the group of nursing, auxiliary nursing, and midwifery instructors, as well as their students (P < .05). Our study shows that there is an urgent need for evaluating education on infection control practices and standard precautions in general, as well as for structured infection control programs among nursing and midwifery staff.

A gram-positive rod with a restriction pattern closely related to the published nucleotide sequence of Mycobacterium mucogenicum was isolated from 6 of 45 units of peripheral blood stem cell products. The source of the contamination was traced to ice cubes used in processing the peripheral blood stem cell products. Substituting reusable ice trays for ice from an ice machine terminated the outbreak.

We describe a pseudo-outbreak of Acinetobacter Iwoffii infection that was recognized early. The pseudo-outbreak involved 16 patients and occurred 3.5 months after the GNS-506 Vitek automated system was introduced in the microbiology laboratory. Prompt confirmation of incomplete use of the automated system's algorithm as the point source of the misidentified A. Iwoffii clinical isolates averted a full outbreak investigation and excess use of infection control resources.

A cluster of clinical isolates of Bordetella bronchiseptica was identified by microbiology laboratory personnel. A clinical and molecular epidemiologic study determined that this cluster represented a pseudo-outbreak due to bacterial contamination of rabbit blood used as a broth culture supplement. This pseudo-outbreak highlights the importance of quality assurance programs in the laboratory.