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Survival of Cephalosporin-Resistant Enterobacteriaceae on Fingers

Published online by Cambridge University Press:  21 June 2016

Anuradha Gunale
Affiliation:
University Institute of Hygiene, Heidelberg, Germany
Heike von Baum
Affiliation:
University Institute of Hygiene, Heidelberg, Germany Institute of Medical Microbiology and Hygiene, Ulm University, Ulm, Germany
Constanze Wendt*
Affiliation:
University Institute of Hygiene, Heidelberg, Germany
*
University Institute of Hygiene, INF 324, 69120 Heidelberg, Germany, ([email protected])

Abstract

Five strains of Enterobacter species (n = 4) and Pantoea species (n = 1) resistant to third-generation cephalosporins and isolated from clusters of 3-25 premature infants and small children and 5 strains (4 Enterobacter strains and 1 Pantoea strain) with the same resistance pattern that were isolated from 1 premature infant or small child each were inoculated on the fingertips of 10 volunteer study participants to test whether survival on fingertips is correlated with horizontal transmission. Although there was no significant difference in survival between the groups of transmitted and sporadic strains, there were significant differences in bacterial survival between the participants.

Type
Concise Communications
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2006

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References

1.D'Agata, E, Venkataraman, L, DeGirolami, PC, et al. Colonization with broad-spectrum cephalosporin-resistant gram-negative bacilli in intensive care units during a non-outbreak period: prevalence, risk factors, and rate of infection. Crit Care Med 1999; 27:10901095.CrossRefGoogle Scholar
2.Honderlick, P, Saheb, F, Cahen, P. Emergence of multidrug resistant Enterobacter cloacae: nosocomial outbreak or change of microbial ecology? Pathol Biol 1999; 47:437439.Google ScholarPubMed
3.Neuwirth, C, Siebor, E, Lopez, J. Outbreak of TEM-24-producing Enterobacter aerogenes in an intensive care unit and dissemination of the extended-spectrum beta-lactamase to other members of the family Enterobacteriaceae. J Clin Microbiol 1996; 34:7679.CrossRefGoogle Scholar
4.Philippon, A, Ben Redjeb, S, Fournier, G. Epidemiology of extended spectrum β-lactamases. Infection 1989; 17:347354.CrossRefGoogle ScholarPubMed
5.Yuan, M, Aucken, H, Hall, LM. Epidermiological typing of klebsiellae with extended-spectrum beta-lactamases from European intensive care units. J Antimicrob Chemother 1998; 41:527539.CrossRefGoogle Scholar
6.Almuneef, MA, Baltimore, RS, Farrel, PA. Molecular typing demonstrating transmission of gram-negative rods in a neonatal intensive care unit in the absence of a recognized epidemic. Clin Infect Dis 2001; 32:220227.CrossRefGoogle Scholar
7.Kartali, G, Tzelepi, E, Pournaras, S, et al. Outbreak of infections caused by Enterobacter cloacae producing the integron-associatedbeta-lactamase IBC-1 in a neonatal intensive care unit of a Greek hospital. Antimicrob Agents Chemother 2002; 46:15771580.CrossRefGoogle Scholar
8.Peters, SM, Bryan, J, Cole, MF. Enterobacterial repetitive intergenic consensus polymerase chain reaction typing of isolates of Enterobacter cloacae from an outbreak of infection in a neonatal intensive care unit. Am J Infect Control 2000; 28:123129.CrossRefGoogle Scholar
9.von Baum, H, Lin, D, Wendt, C. Prevalence of colonisation with third-generation cephalosporin resistant Enterobacteriaceae in ICU patients of Heidelberg University Hospitals. Clin Microbiol Infect 2004; 10:436440.CrossRefGoogle ScholarPubMed
10.Knittle, MA, Eitzman, DV, Baer, H. Role of hand contamination of personnel in the epidemiology of gram-negative nosocomial infection. J Pediatr 1975; 86:433437.CrossRefGoogle Scholar
11.Panhotra, BR, Bhardwaj, G, Naqash, HU. Isolation and survival on gentamicin-resistant Enterobacter aerogenes on finger tips of hospital personnel. Zentralbl Hyg Umweltmed 1989; 188:331335.Google ScholarPubMed
12.van Nierop, WH, Duse, AG, Stewart, RG. Molecular epidemiology of an outbreak of Enterobacter cloacae in the neonatal intensive care unit of a provincial hospital in Gauteng, South Africa. J Clin Microbiol 1998; 36: 30853087.CrossRefGoogle ScholarPubMed
13.Yu, WL, Cheng, HS, Lin, HC. Outbreak investigation of nosocomial Enterobacter cloacae bacteraemia in a neonatal intensive care unit. Scand J Infect Dis 2000; 32:293298.Google Scholar
14.Pfaller, M, Hollis, RJ, Sader, HS. PFGE of chromosomal DNA. In: Isenberg, HD, ed. Clinical Microbiology Procedures Handbook. Washington: American Society for Microbiology; 1992:10.15.cGoogle Scholar
15.Gonzalas-Vertiz, A, Alcantar-Curiel, D, Cuauhtli, M, et al. Multiresistant extended-spectrum β-lactamase-producing Klebsiella pneumoniae causing an outbreak of nosocomial bloodstream infection. Infect Control Hosp Epidemiol 2001; 22:723725.CrossRefGoogle Scholar
16.Flynn, DM, Weinstein, RA, Nathan, C. Patients endogenous flora as the source of nosocomial enterobacter in cardiac surgery. J Infect Dis 1987; 156:363368.CrossRefGoogle Scholar
17.van den Berg, RW, Claahsen, HL, Niessen, M. Enterobacter cloacae outbreak in the NICU related to disinfected thermometers. J Hosp Infect 2000; 45:2934.CrossRefGoogle ScholarPubMed
18.Tresoldi, AT, Padoveze, MC, Trabasso, P, et al. Enterobacter cloacae sepsis outbreak in a newborn unit caused by contaminated total parenteral nutrition solution. Am J Infect Control 2000; 28:258261.CrossRefGoogle Scholar
19.Fryklund, B, Tullus, K, Burman, LG. Survival on skin and surfaces of epidemic and non-epidemic strains of enterobacteria from neonatal special care units. J Hosp infect 1995; 29:201208.CrossRefGoogle ScholarPubMed