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Protein Fingerprinting for the Determination of Relatedness in Acinetobacter calcoaceticus subspecies anitratus Isolated from Patients in a Surgical Intensive Care Unit

Published online by Cambridge University Press:  02 January 2015

Joel E. Mortensen ,
Mark T. LaRocco ,
Bret Steiner  and
Bruce Ribner
Joel E. Mortensen*
Affiliation:
Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, Houston, Texas
Mark T. LaRocco
Affiliation:
Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, Houston, Texas
Bret Steiner
Affiliation:
Department of Medical Microbiology, University of Wisconsin Medical School, Madison, Wisconsin
Bruce Ribner
Affiliation:
Program in Infectious Diseases, University of Texas Health Science Center, Houston, Texas
*
Department of Laboratories, St. Christopher's Hospital for Children, Philadelphia, PA 19133
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Abstract

The use of protein fingerprinting for the establishment of relatedness among isolates of Acinetobacter calcoaceticus subspecies anitratus, isolated from patients in a surgical intensive care unit was examined. Poly-acrylamide gel electrophoresis was used to analyze the cellular proteins from whole cell lysates of 14 intensive care unit A calcoaceticus subspecies anitratus isolates and 11 control strains. Antimicrobial susceptibilities and plasmid profiles were also determined for all isolates. All intensive care unit A calcoaceticus subspecies anitratus isolates exhibited identical cellular protein fingerprints, no detectable plasmids, and minimum inhibitory concentrations within ±1 log2 dilution of the mode value for each of the antimicrobial agents tested. The other clinical isolates demonstrated a range of antimicrobial susceptibilities, various numbers and sizes of plasmids, and distinctly different protein fingerprints. These data indicate that protein fingerprinting may be useful as an epidemiologic tool in A calcoaceticus subspecies anitratus outbreaks and this method deserves further study.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 8 , Issue 12 , December 1987 , pp. 512 - 515
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1987

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