Hostname: page-component-586b7cd67f-g8jcs Total loading time: 0 Render date: 2024-11-30T21:48:08.906Z Has data issue: false hasContentIssue false
  • English
  • Français

Nosocomial Outbreak of Pseudomonas aeruginosa Endophthalmitis

Published online by Cambridge University Press:  21 June 2016

I. Mateos ,
R. Valencia ,
M. J. Torres ,
A. Cantos ,
M. Conde  and
J. Aznar
I. Mateos
Affiliation:
Service of Clinical Microbiology, University Hospitals Virgen del Rocío, Seville, Spain
R. Valencia
Affiliation:
Service of Preventive Medicine, University Hospitals Virgen del Rocío, Seville, Spain
M. J. Torres
Affiliation:
University of Seville, University Hospitals Virgen del Rocío, Seville, Spain
A. Cantos
Affiliation:
Service of Clinical Microbiology, University Hospitals Virgen del Rocío, Seville, Spain
M. Conde
Affiliation:
Service of Preventive Medicine, University Hospitals Virgen del Rocío, Seville, Spain
J. Aznar*
Affiliation:
Service of Clinical Microbiology, University Hospitals Virgen del Rocío, Seville, Spain University of Seville, University Hospitals Virgen del Rocío, Seville, Spain
*
Servicio de Microbiología, Hospitales Universitarios Virgen del Rocío, Avda. Manuel Siurot, s/n 41013 Sevilla, Spain ([email protected])
Get access Rights & Permissions [Opens in a new window]

Abstract

We describe an outbreak of nosocomial endophthalmitis due to a common source, which was determined to be trypan blue solution prepared in the hospital's pharmacy service. We assume that viable bacteria probably gained access to the trypan blue stock solution during cooling after autoclaving. The temporal cluster of Pseudomonas aeruginosa endophthalmitis was readily perceived on the basis of clinical and microbiological findings, and an exogenous source of contamination was unequivocally identified by means of DNA fingerprinting.

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 27 , Issue 11 , November 2006 , pp. 1249 - 1251
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Pier, GB, Ramphal, R. Pseudomonas aeruginosa . In: Mandell, GL, Bennett, JE, Dolin, R, eds. Mandell, Douglas and Bennett's Principles and Practice of Infectious Diseases. 6th ed. New York: Churchill Livingston; 2005:2587–2611.Google Scholar
2. Busbee, BG. Advances in knowledge and treatment: an update on endophthalmitis. Current Opin Ophthalmol 2004; 15:232–237.CrossRefGoogle ScholarPubMed
3. Callegan, MC, Engelbert, M, Parke, DW II, Jet, BD, Gilmore, MS. Bacterial endophthalmitis: epidemiology, therapeutics, and bacterium-host interactions. Clin Microbiol Rev 2002; 15:111–124.CrossRefGoogle ScholarPubMed
4. Cruciani, M, Malena, M, Amalfitano, G, Monti, P, Bonomi, L. Molecular epidemiology in a cluster of cases of postoperative Pseudomonas aeruginosa endophthalmitis. Clin Infect Dis 1998; 26:330–333.CrossRefGoogle Scholar
5. Hanscom, TA. Postoperative endophthalmitis. Clin Infect Dis 2004; 38:542–546.Google Scholar
6. Taban, M, Behrens, A, Newcomb, RL, Nobe, MY, Saedi, G, Sweet, PM, et al. Acute endophtalmitis following cataract surgery: a systematic review of the literature. Arch Ophthalmol 2005; 123:613–620.CrossRefGoogle Scholar
7. Swaddiwudhipong, W, Linlawan, P, Prasantong, R, Kiphati, R, Wongwat-charapaiboon, P. A report of an outbreak of postoperative endophthalmitis. J Med Assoc Thai 2000; 83:902–907.Google Scholar
8. Martin-Lozano, D, Cisneros, JM, Becerril, B, et al. Comparison of a repetitive extragenic palindromic sequence-based PCR method and clinical and microbiological methods for determining strain sources in cases of nosocomial Acinetobacter baumannii bacteriemia. J Clin Microbiol 2002; 40:4571–4575.CrossRefGoogle Scholar
9. Woods, CR, Versalovic, J, Koeuth, T, Lupski, JR. Whole-cell repetitive element sequence–based polymerase chain reaction allows rapid assessment of clonal relationships of bacterial isolates. J Clin Microbiol 1993; 31:1927–1931.Google Scholar
10. Laureano, JS, Corroneo, MT. Crystalline lens capsule staining with trypan blue. J Cataract Refract Surg 2004; 30:2046–2049.CrossRefGoogle ScholarPubMed
11. Saini, JS, Jain, AK, Sukhija, J, Gupta, P, Saroha, V. Anterior and posterior capsulorhexis in pediatric cataract surgery with or without trypan blue dye: randomized prospective clinical study. J Cataract Refract Surg 2003; 29:1732–1737.Google Scholar
12. Morel, PC, Roubi, N, Talon, DR, Betrand, X. Contamination of trypan blue with Burkholderia cepacia in a cornea bank. Infect Control Hosp Epidemiol 2003; 24:198–201.Google Scholar
13. Doit, C, Simon, AM, Ferroni, A, et al. Outbreak of Bukholderia cepacia bacteraemia in a pediatric hospital due to contamination of lipid emulsion stoppers. J Clin Microbiol 2004; 42:2227–2230.CrossRefGoogle Scholar