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Molecular Typing of Agrobacterium Species Isolates From Catheter-Related Bloodstream Infections

Published online by Cambridge University Press:  02 January 2015

Giovanni M. Giammanco ,
Sarina Pignato ,
Carmelita Santangelo ,
Patrick A. D. Grimont ,
Francine Grimont  and
Giuseppe Giammanco
Giovanni M. Giammanco*
Affiliation:
Dipartimento di Igiene e Microbiologia, Università di Palermo, Palermo, Italy
Sarina Pignato
Affiliation:
Dipartimento G.F. Ingrassia-Igiene e Sanità Pubblica, Università di Catania, Catania, Italy
Carmelita Santangelo
Affiliation:
Servizio di Patologia Clinica, Sezione di Batteriologia, Azienda Ospedali Vittorio Emanuele, Ferrarotto, Santo Bambino, Catania, Italy
Patrick A. D. Grimont
Affiliation:
Unité de Biodiversité des Bactéries Pathogènes Emergentes, Unité INSERM 389, Institut Pasteur, Paris, France
Francine Grimont
Affiliation:
Unité de Biodiversité des Bactéries Pathogènes Emergentes, Unité INSERM 389, Institut Pasteur, Paris, France
Giuseppe Giammanco
Affiliation:
Dipartimento G.F. Ingrassia-Igiene e Sanità Pubblica, Università di Catania, Catania, Italy
*
Dipartimento di Igiene e Microbiologia, Università di Palermo, via del Vespro 133, 90127 Palermo, Italy
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Abstract

Agrobacterium isolates from intravenous catheters of three hospitalized patients were initially identified as A. tumefaciens, but inability to produce 3-ketolactose revealed that two of them were A. vitis. However, rDNA analysis correlated all of the isolates to A. tumefaciens. Pulsed-field gel electrophoresis analysis ascertained the nosocomial transmission of the infection.

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 25 , Issue 10 , October 2004 , pp. 885 - 887
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2004 

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References

1. Hülse, M, Johnson, S, Fernen, P. Agrobacterium infections in humans: experience at one hospital and review. Clin Infect Dis 1993;16:112–117.10.1093/clinids/16.1.112Google Scholar
2. Amaya, RA, Edwards, MS. Agrobacterium radiobacter bacteremia in pediatric patients: case report and review. Pediatr Infect Dis f 2003;22:183–186.Google Scholar
3. Paphitou, NI, Rolston, KV. Catheter-related bacteremia caused by Agrobacterium radiobacter in a cancer patient: case report and literature review. Infection 2003;31:421–424.10.1007/s15010-003-3175-5Google Scholar
4. Potvliege, C, Vanhuynegem, L, Hansen, W. Catheter infection caused by an unusual pathogen, Agrobacterium radiobacter . J Clin Microbiol 1989;27:2120–2122.10.1128/jcm.27.9.2120-2122.1989Google Scholar
5. Kersters, K, De Ley J., Genus III: Agrobacterium. In: Krieg, NR, Holt, JG, eds. Bergey's Manual of Systematic Bacteriology, vol. I. Baltimore: Williams & Wilkins; 1984:244–254.Google Scholar
6. Ophel, K, Kerr, A. Agrobacterium vitis sp. nov. for strains of Agrobacterium biovar 3 from grapevines. Int J Syst Bacterial 1990;40:236–241.Google Scholar
7. Sawada, H, leki, H, Oyaizu, H, Matsumoto, S. Proposal for rejection of Agrobacterium tumefaciens and revised descriptions for the genus Agrobacterium and for Agrobacterium radiobacterand Agrobacterium rhi-zogenes . Int J Syst Bacterial 1993;43:694–702.10.1099/00207713-43-4-694Google Scholar
8. Freney, J, Gruer, LD, Bormstein, N, et al. Septicemia caused by Agrobacterium sp . J Clin Microbiol 1985;22:683–685.Google Scholar
9. Edmond, MB, Riddler, SA, Baxter, CM, Wicklund, BM, Pasculle, AW. Agrobacterium radiobacter. a recently recognized opportunistic pathogen. Clin Infect Dis 1993;16:388–391.Google Scholar
10. Funke, G, Monnet, D, deBernardis, C, von Graevenitz, A, Freney, J. Evaluation of the VITEK 2 system for rapid identification of medically relevant gram-negative rods. J Clin Microbiol 1998;36:1948–1952.10.1128/JCM.36.7.1948-1952.1998Google Scholar
11. O'Hara, CM, Miller, JM. Evaluation of the Vitek 2ID-GNB assay for identification of members of the family Enterobacteriaceae and other nonen-teric gram-negative bacilli and comparison with the Vitek GNI+ card. J Clin Microbiol 2003;41:2096–2101.Google Scholar
12. Ainor, D, Frimodt-Moller, N, Espersen, F, Frederiksen, W. Infections with the unusual human pathogen Agrobacterium species and Ochrobactrum anthropi . Clin Infect Dis 1994;18:914–920.Google Scholar