Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-11-28T00:50:36.150Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular Epidemiology of Endemic Ciprofloxacin-Susceptible and -Resistant Enterobacteriaceae

Published online by Cambridge University Press:  21 June 2016

Y.C. Yee ,
RR Muder ,
M.H. Hsieh  and
T.C. Lee
Y.C. Yee
Affiliation:
Special Pathogens Laboratory, Veterans Affairs Medical Center and the Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania
RR Muder*
Affiliation:
Infectious Disease Section, Veterans Affairs Medical Center and the Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania
M.H. Hsieh
Affiliation:
Special Pathogens Laboratory, Veterans Affairs Medical Center and the Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania
T.C. Lee
Affiliation:
Special Pathogens Laboratory, Veterans Affairs Medical Center and the Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania
*
Infectious Disease Section, VA Medical Center, University Drive C, Pittsburgh, PA 15240
Get access Rights & Permissions [Opens in a new window]

Abstract

Objectives:

To elucidate the epidemiology of widespread ciprofloxacin resistance in our Veterans Affairs medical center using whole cell DNA analysis.

Design:

In vitro study of ciprofloxacin resistant and susceptible Enterobacteriaceae isolated during the course of a clinical epidemiologic study of quinolone resistance.

Setting:

Veterans Affairs Medical Center with acute care and long-term care divisions.

Results:

We examined 40 ciprofloxacin-resistant strains of Serratia marcescens, Proteus mirabilis, and Providencia stuartii using restriction endonuclease analysis of whole cell DNA and compared them with concomitantly isolated ciprofloxacin sensitive strains. We sought to determine whether resistant strains were identical to susceptible strains, indicating in vivo emergence of resistant strains from susceptible strains, and whether resistant strains were shared among patients. All 26 ciprofloxacin-resistant S marcestens isolates shared a single ecoRI restriction pattern. Multiple patterns were seen in the ciprofloxacin-susceptible S marcescens isolates; however, several isolates had a pattern matching that of the resistant isolates. Similar results were seen among the P mirabilis isolates. Three different ecoRI patterns were found among the ciprofloxacin-resistant P stuartii isolates; none matched those found among the susceptible isolates. The frequency of spontaneous emergence of ciprofloxacin resistance in susceptible S marcescens strains with restriction pattern matching that of the resistant strains was significantly higher than that of nonmatching strains.

Conclusions:

Ciprofloxacin-resistant strains of Enterobacteriaceae became widespread within a short period of time. Resistant strains of S marcestens and P mirabilis arose from endemic susceptible strains. The resistant strain of S marcescens appeared to arise from a susceptible strain with a relatively high frequency of spontaneous ciprofloxacin resistance.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 13 , Issue 12 , December 1992 , pp. 706 - 710
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Muder, RR, Brennen, C, Goetz, AM, Wagener, MM, Rihs, JD. Association with prior fluoroquinolone therapy of widespread ciprofloxacin resistance among gram-negative isolates in a veterans affairs medical center. Antimicrob Agents Chemother. 1991;35:256–258.CrossRefGoogle Scholar
2. Ausbel, FM, Brent, R, Kingston, RE, Moore, DD, Seidman, JG, Smith, JA, Struhl, K. Short Protocols in Molecular Biology. New York. New York John Wilev and Sons: 1989.Google Scholar
3. Farber, BE; Yee, YC. High-level aminoglycoside resistance mediated by aminoglycoside-modifying enzymes among viridans streptococci; implications for the therapy for endocarditis. J Infect Dis. 1987;155:948–953.Google Scholar
4. Warren, JW. Providencia stuartii: a common cause of antibiotic-resistant bacteria in patients with long-term indwelling catheters. Rev Infect Dis. 1986;8:61–67.Google Scholar
5. Kocka, FE, Srinivasan, S, Mowjood, M, Kantor, HS. Nosocomial multiply resistant Providencia stuatiii: a long-term outbreak with multiple biotypes and serotynes at one hospital. J Clin Microbiol. 1980;11:167–169.Google Scholar
6. Watanbe, M, Kotera, Y, Yosue, K, Inoue, M, Mitsuhashi, S. In vitro emergence of quinolone-resistant mutants of Escherichia coli, Enterobacter cloacae, and Serratia marcescens . Antimicrob Agents Chemother. 1990;34:173–175.CrossRefGoogle Scholar