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Molecular Characterization of Vancomycin-Resistant Enterococci from Clinical and Surveillance Specimens

Published online by Cambridge University Press:  21 June 2016

Ji Young Huh ,
Wee Gyo Lee  and
Hye Young Jin
Ji Young Huh
Affiliation:
Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Ajou University School of Medicine, Suwon, South Korea
Wee Gyo Lee*
Affiliation:
Departments of Laboratory Medicine, Ajou University School of Medicine, Suwon, South Korea
Hye Young Jin
Affiliation:
Hospital Infection Control, Ajou University School of Medicine, Suwon, South Korea
*
Department of Laboratory Medicine, Ajou University Hospital, San 5, Wonchun-Dong, Youngtong-Gu, Suwon 442-749, South Korea, ([email protected])
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Extract

Objective:

To compare the molecular characteristics of infection-derived (ID) isolates and intestinal colonization–derived (ICD) isolates of vancomycin-resistant enterococci (VRE) recovered from hospitalized patients.

Design.

A 12-month prospective cohort study.

Setting.

A 1,000-bed teaching facility.

Methods.

From January through December 2004, a total of 30 pairs of vanA-containing enterococcal isolates were collected from patients admitted to a teaching hospital in South Korea. Each pair comprised an ID and an ICD VRE isolate from the same patient. All VRE isolates were investigated on the basis of SmaI-restricted pulsed-field gel electrophoresis (PFGE) pattern, Tn1546type, and presence of the esp gene, including A and C repeat number variation.

Results.

Members of 19 pairs (63%) of VRE isolates were genetically indistinguishable from each other. The 11 patients for whom the molecular characteristics of the ID isolates differed from those of the ICD isolates had longer durations of hospitalization and intensive care unit (ICU) stay, compared with the other 19 patients.

Conclusions.

These findings suggest the longer durations of hospitalization and ICU stay may be possible risk factors for colonization with multiple clones of VRE.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 27 , Issue 10 , October 2006 , pp. 1076 - 1080
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2006

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