Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-03T05:28:34.015Z Has data issue: false hasContentIssue false
  • English
  • Français

Investigation of an Apparent Cluster of Klebsiella pneumoniae Bacteremias Using Random Amplified Polymorphic DNA Analysis

Published online by Cambridge University Press:  02 January 2015

James C. Hurley ,
Edward G. Russell ,
Glenys Harrington  and
W. John Spicer
Get access Rights & Permissions [Opens in a new window]

Abstract

A cluster of bacteremia episodes with Klebsiella pneumoniae was noted in patients in a hematology-oncology ward during a 3-week period. Random amplified polymorphic DNA (RAPD) analysis, a novel technique for generating chromosomal fingerprints from bacterial isolates, was used as an aid to the epidemiological investigation of this cluster. For each of the two patients from whom multiple isolates had been obtained, identical RAPD patterns were observed in the serial isolates, even for a patient where the isolates had different biotypes. Isolates from different patients gave distinct patterns. Random amplified polymorphic DNA was found to be a useful typing technique for this cluster of K pneumonia bacteremias.

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 17 , Issue 11 , November 1996 , pp. 743 - 745
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Eisen, D, Russell, EG, Tymms, M, Roper, EJ, Grayson, ML, Turnidge, J. Random amplified polymorphic DNA and plasmid analysis used in the investigation of an outbreak of multiresistant Klebsiella pneumoniae . J Clin Microbiol 1995;33:713–717.CrossRefGoogle ScholarPubMed
2. Williams, JGK, Kubelik, AR, Livak, KJ, Rafalski, JA, Tingey, SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 1990;18:6531–6535.CrossRefGoogle ScholarPubMed
3. Welsh, JW, McClelland, M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 1990;18:7213–7218.CrossRefGoogle ScholarPubMed
4. Mazurier, SI, Wernars, K. Typing of Listeria strains by random amplified polymorphic DNA. Res Microbiol 1992;143:499–505.CrossRefGoogle Scholar
5. Versalovic, J, Woods, CR Jr, Georghiou, PR, Hamill, RJ, Lupski, JR. DNA-based identification and epidemiological typing of bacterial pathogens. Arch Pathol Lab Med 1993;117:1088–1098.Google Scholar
6. Bingen, E, Denamur, E, Lambert-Zechovsky, N, Brahimi, N, Lakany, ME, Elion, J. Rapid genotyping shows absence of cross-contamination in Enterobacter cloacae nosocomial infections. J Hosp Infect 1992;21:95–101.Google Scholar
7. Versalovic, J, Koeuth, T, Lupski, JR. Distribution of repetitive sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 1991;19:6823–6831.CrossRefGoogle ScholarPubMed
8. Georghiou, PR, Hamill, RJ, Wright, CE, et al. Molecular epidemiology of infections due to Enterobacter aerogenes: identification of hospital outbreak associated strains by molecular techniques. Clin Infect Dis 1995;20:84–94.CrossRefGoogle ScholarPubMed
9. Maslow, JN, Brecher, SM, Adams, KS, Durbin, A, Loring, S, Arbeit, RD. Relationship between indole production and differentiation of Klebsiella species: indole-positive and -negative isolates of Klebsiella determined to be clonal. J Clin Microbiol 1993;31:2000–2003.CrossRefGoogle ScholarPubMed