Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-08T09:27:46.061Z Has data issue: false hasContentIssue false
  • English
  • Français

Genotypic Analysis by 27A DNA Fingerprinting of Candida albicans Strains Isolated During an Outbreak in a Neonatal Intensive Care Unit

Published online by Cambridge University Press:  02 January 2015

Stefania Boccia ,
Brunella Posteraro ,
Marilena La Sorda ,
Giovanni Vento ,
Piero Giuseppe Matassa ,
Alessia Tempera ,
Stefano Petrucci  and
Giovanni Fadda
Stefania Boccia
Affiliation:
Institute of Microbiology, Università Cattolica del Sacro Cuore, Rome, Italy
Brunella Posteraro*
Affiliation:
Institute of Microbiology, Università Cattolica del Sacro Cuore, Rome, Italy
Marilena La Sorda
Affiliation:
Institute of Microbiology, Università Cattolica del Sacro Cuore, Rome, Italy
Giovanni Vento
Affiliation:
Neonatal Intensive Care Unit, Università Cattolica del Sacro Cuore, Rome, Italy
Piero Giuseppe Matassa
Affiliation:
Neonatal Intensive Care Unit, Università Cattolica del Sacro Cuore, Rome, Italy
Alessia Tempera
Affiliation:
Neonatal Intensive Care Unit, Università Cattolica del Sacro Cuore, Rome, Italy
Stefano Petrucci
Affiliation:
Neonatal Intensive Care Unit, Università Cattolica del Sacro Cuore, Rome, Italy
Giovanni Fadda
Affiliation:
Institute of Microbiology, Università Cattolica del Sacro Cuore, Rome, Italy
*
Institute of Microbiology, Università Cattolica del Sacro Cuore, L. go F. Vito, 1-00168 Rome, Italy
Get access Rights & Permissions [Opens in a new window]

Abstract

We describe an outbreak of Candida albicans systemic infection involving five premature infants in a neonatal intensive care unit. Molecular and epidemiologic characterization of all C. albicans isolates was performed by DNA fingerprinting with the 27A probe. This genotypic analysis demonstrated that the isolates were identical, providing evidence for the circulation of a unique C. albicans strain.

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 23 , Issue 5 , May 2002 , pp. 281 - 284
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2002

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Hedderwick, SA, Lyons, MJ, Liu, M, Vazquez, JA, Kauffman, CA. Epidemiology of yeast colonization in the intensive care unit. Eur J Clin Microbiol Infect Dis 2000;19:663–670.CrossRefGoogle ScholarPubMed
2.Ruiz-Diez, B, Martinez, V, Alvarez, M, Rodriguez-Tudela, JL, Martinez-Suarez, JV. Molecular tracking of Candida albicans in a neonatal intensive care unit: long-term colonizations versus catheter-related infections. J Clin Microbiol 1997;35:3032–3036.CrossRefGoogle Scholar
3.Fowler, SL, Rhoton, B, Springer, SC, Messer, SA, Hollis, RJ, Pfaller, MA. Evidence for person-to-person transmission of Candida lusitaniae in a neonatal intensive-care unit. Infect Control Hosp Epidemiol 1998;19:343–345.CrossRefGoogle Scholar
4.Pfaller, MA. Epidemiology of fungal infections: the promise of molecular typing. Clin Infect Dis 1995;20:1535–1539.CrossRefGoogle ScholarPubMed
5.Chapman, LC, Faix, RG. Persistently positive cultures and outcome in invasive neonatal candidiasis. Pediatr Infect Dis J 2000;19:822–827.CrossRefGoogle ScholarPubMed
6.Warren, NG, Hazen, KC. Candida, Cryptococcus, and other yeasts of medical importance. In: Murray, PR, Baron, EJ, Pfaller, MA, Tenover, FC, Yolken, RH, eds. Manual of Clinical Microbiology, 7th ed. Washington, DC: ASM Press; 1999:1184–1199.Google Scholar
7.National Committee for Clinical Laboratory Standards. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts. Approved Standard, M27-A Methodologies. Villanova, PA: National Committee for Clinical Laboratory Standards; 1997.Google Scholar
8.Morace, G, Sanguinetti, M, Posteraro, B, Lo Cascio, G, Fadda, G. Identification of various medically important Candida species in clinical specimens by PCR-restriction enzyme analysis. J Clin Microbiol 1997; 35:667–672.CrossRefGoogle ScholarPubMed
9.Scherer, S, Stevens, DA. A Candida albicans dispersed, repeated gene family and its epidemiologic applications. Proc Natl Acad Sci U S A 1998;85:1452–1456.CrossRefGoogle Scholar
10.Pujol, C, Joly, S, Lockhart, SR, Noel, S, Tybayrenc, M, Soll, DR. Parity among the randomly amplified polymorphic DNA method, multilocus enzyme electrophoresis, and Southern blot hybridization with the moderately repetitive DNA probe Ca3 for fingerprinting C. albicans. J Clin Microbiol 1997;35:2348–2358.CrossRefGoogle Scholar
11.Leung, WK, Dassanayake, RS, Yau, JYY, Jin, LJ, Yam, WC, Samaranayake, L. Oral colonization, phenotypic, and genotypic profiles of Candida species in irradiated, dentate, xerostomic nasopharyngeal carcinoma survivors. J Clin Microbiol 2000;38:2219–2226.CrossRefGoogle ScholarPubMed
12.Faix, RG, Finkel, DJ, Andersen, RD, Hostetter, MK. Genotypic analysis of a cluster of systemic Candida albicans infections in a neonatal intensive care unit. Pediatr Infect Dis J 1995;14:1063–1068.CrossRefGoogle Scholar
13.Rangel-Frausto, MS, Wiblin, T, Blumberg, HM, et al. National epidemiology of mycoses survey (NEMIS): variations in rates of bloodstream infections due to Candida species in seven surgical intensive care units and six neonatal intensive care units. Clin Infect Dis 1999;29:253–258.CrossRefGoogle ScholarPubMed
14.Pfaller, MA. Nosocomial candidiasis: emerging species, reservoirs, and modes of transmission. Clin Infect Dis 1996;22:S89–S94.CrossRefGoogle ScholarPubMed
15.Huang, YC, Lin, TY, Peng, HL, Wu, JH, Chang, HY, Leu, HS. Outbreak of Candida albicans fungaemia in a neonatal intensive care unit. Scand J Infect Dis 1998;30:137–142.Google Scholar
16.Waggoner-Fuontain, LA, Whit Walker, M, Hollis, R, et al. Vertical and horizontal transmission of unique Candida species to premature newborns. Clin Infect Dis 1996; 22:803–808.CrossRefGoogle Scholar