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Experimental Evaluation of an Automated Endoscope Reprocessor With In Situ Generation of Peracetic Acid for Disinfection of Semicritical Devices

Published online by Cambridge University Press:  21 June 2016

Syed A. Sattar ,
Richard J. Kibbee ,
Jason A. Tetro  and
Tony A. Rook
Syed A. Sattar*
Affiliation:
Centre for Research on Environmental Microbiology, University of Ottawa, Ottawa, Ontario
Richard J. Kibbee
Affiliation:
Centre for Research on Environmental Microbiology, University of Ottawa, Ottawa, Ontario
Jason A. Tetro
Affiliation:
Centre for Research on Environmental Microbiology, University of Ottawa, Ottawa, Ontario
Tony A. Rook
Affiliation:
Steris Corporation, Mentor, Ohio
*
Centre for Research on Environmental Microbiology, University of Ottawa, 451 Smyth Road, Ottawa, Ontario, CanadaK1H 8M5 ([email protected])
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Abstract

Objective.

To evaluate the effectiveness of a high-level disinfection solution generated inside an endoscope processing system for decontaminating external and internal surfaces of experimentally contaminated heat-sensitive medical devices.

Methods.

The American Society for Testing and Materials Simulated-Use Test protocol (E1837-02), which incorporates a soil load in each inoculum, was used to evaluate the efficacy of the system when processing 4 common types of endoscopes contaminated separately with 5 types of nosocomial pathogens: Pseudomonas aeruginosa (ATCC 15442), spores of Clostridium difficile (ATCC 9689), a glutaraldehyde-resistant strain of Mycobacterium chelonae, a vancomycin-resistant strain of Enterococcus faecalis, and a methicillin-resistant strain of Staphylococcus aureus. Rinse solution samples from channels and from surfaces of the processed endoscopes were tested for any microbicidal residues.

Results.

For all organisms tested, the baseline level of contamination of the endoscopes ranged from 5 log10 to greater than 7 log10 at each external surface site and internal channel. All tests showed reductions in viability of the test organisms to undetectable levels. All rinse solution samples from external and internal sites of the endoscopes proved to be free of any residual microbicidal activity.

Conclusions.

The endoscope reprocessor, with its processor-generated high-level disinfection solution, successfully reduced the numbers of selected, clinically relevant pathogens to undetectable levels both in the channels and on the outside surfaces of the 4 representative endoscopes tested in this study.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 27 , Issue 11 , November 2006 , pp. 1193 - 1199
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2006

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