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Tetrasodium EDTA as a Novel Central Venous Catheter Lock Solution Against Biofilm

Published online by Cambridge University Press:  21 June 2016

Steven L. Percival ,
Peter Kite ,
Kerrie Eastwood ,
Ricardo Murga ,
Janice Carr ,
Matthew J. Arduino  and
Rodney M. Donlan
Steven L. Percival
Affiliation:
Department of Microbiology, Leeds Teaching Hospitals, Leeds, United Kingdom Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia
Peter Kite
Affiliation:
Department of Microbiology, Leeds Teaching Hospitals, Leeds, United Kingdom
Kerrie Eastwood
Affiliation:
Department of Microbiology, Leeds Teaching Hospitals, Leeds, United Kingdom
Ricardo Murga
Affiliation:
Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia
Janice Carr
Affiliation:
Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia
Matthew J. Arduino
Affiliation:
Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia
Rodney M. Donlan*
Affiliation:
Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia
*
Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, 1600 Clifton Road, N.E., Mail Stop C-16, Atlanta, GA 30333.[email protected]
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Abstract

Background:

Central venous catheter (CVC)-related bloodstream infections (BSIs) are known to increase rates of morbidity and mortality in both inpatients and outpatients, including hematology-oncology patients and those undergoing hemodialysis or home infusion therapy. Biofilm-associated organisms on the lumens of these catheters have reduced susceptibility to antimicrobial chemotherapy. This study tested the efficacy of tetrasodium EDTA as a catheter lock solution on biofilms of several clinically relevant microorganisms.

Methods:

Biofilms of Staphylococcus epidermidis, methicillin-resistant S. aureus, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, and Candida albicans were grown to levels of approximately 1 × 105 colony-forming units (CFU)/cm−1 on CVC segments in a model system, then subjected to the tetrasodium EDTA lock treatment.

Results:

Comparisons of biofilms before and after exposure to the 40-mg/mL−1 tetrasodium EDTA lock for 21 hours showed that the biofilm viable cell counts of all organisms tested were significantly reduced (P < .05) after exposure to the treatment.

Conclusion:

Antimicrobial lock treatment using 40 mg/mL−1 of tetrasodium EDTA for at least 21 hours could significantly reduce or potentially eradicate CVC-associated bio-films of clinically relevant microorganisms (Infect Control Hosp Epidemiol 2005;26:515-519).

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 26 , Issue 6 , June 2005 , pp. 515 - 519
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2005

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