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Polymerase Chain Reaction Identification of Coagulase-Negative Staphylococci and of Strain Diversity and Spread of Staphylococcus epidermidis in a Major Medical Center in Lebanon

Published online by Cambridge University Press:  21 June 2016

Inaya M. Abdallah ,
George E Araj ,
Ghassan M. Matar ,
George Abdelnour ,
Marwan Uwaydah  and
Alexander M. Abdelnoor
Inaya M. Abdallah
Affiliation:
Department of Microbiology and Immunology, American University of Beirut Medical Center, Lebanon
George E Araj
Affiliation:
Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, Lebanon
Ghassan M. Matar
Affiliation:
Department of Microbiology and Immunology, American University of Beirut Medical Center, Lebanon
George Abdelnour
Affiliation:
Hôpital Notre Dame Des Secours, Byblos, Lebanon
Marwan Uwaydah
Affiliation:
Department of Microbiology and Immunology, American University of Beirut Medical Center, Lebanon
Alexander M. Abdelnoor*
Affiliation:
Department of Microbiology and Immunology, American University of Beirut Medical Center, Lebanon
*
Department of Microbiology and Immunology, American University of Beirut, PO Box 11-0236, Riad El-Solh, Beirut 11072020, Lebanon ([email protected])
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Abstract

A 2-step polymerase chain reaction (PCR) assay and random amplification of polymorphic DNA (RAPD) analysis, respectively, were assessed to identify coagulase-negative staphylococci organisms to the species level and to determine the strain diversity and spread of Staphylococcus epidermidis, the most frequently isolated species, in a medical center in Beirut, Lebanon. Our data indicated that PCR was faster and was more efficient in identifying S. epidermidis isolates than is conventional biochemical testing. RAPD analysis have shown that S. epidermidis strains were scattered across the different clinical services, demonstrating various clusters of infection in the medical center.

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 27 , Issue 7 , July 2006 , pp. 781 - 783
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2006

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References

1. Arbeit, RD. Laboratory procedures for epidemiological analysis. In: Crossley, KB, Archer, GL, eds. The Staphylococci in Human Disease. New York; Churchill Livingston; 1997:253–286.Google Scholar
2. Diekema, DJ, Pfaller, MA, Schmitz, FJ, et al. Survey of infections due to Staphylococcus species: frequency of occurrence and antimicrobial susceptibility of isolates collected in the United States, Canada, Latin America, Europe, and the Western Pacific region for the sentry antimicrobial surveillance program. 1997-1999. Clin Infect Dis 2001; 32(Suppl):114–132.Google Scholar
3. Matar, GM, Hébert, GA, Uwaydah, , M, . Characteristics of nosocomial versus outpatient isolates of coagulase-negative staphylococci at a Medical Center in Beirut Lebanon. Leb Sci Bull 1995; 8:63–75.Google Scholar
4. Huebner, J, Goldmann, DA. Coagulase-negative staphylococci: role as pathogens. Ann Rev Med 1999; 50:223–236.Google Scholar
5. Ronald, A. The etiology of urinary tract infection: traditional and emerging pathogens. Am J Med Suppl 2002; 113:14S–19S.Google Scholar
6. Peters, G, Von Eiff, C, Herrmann, M. The changing pattern of coagulase-negative staphylococci as infectious pathogens. Curr Opin Infect Dis 1995; 8(Suppl 1):S12–S19.Google Scholar
7. Patrick, CC. Coagulase-negative staphylococci: pathogens with increasing clinical significance. J Pediatr 1990; 116:497–507.Google Scholar
8. Martin, MA, Pfaller, A, Wenzel, RP. Coagulase-negative staphylococcal bacteremia. Ann Intern Med 1989; 110:9–16.Google Scholar
9. Pfaller, MA, Herwaldt, LA. Laboratory, clinical, and epidemiological aspects of coagulase-negative staphylococci. Clin Microbiol Rev 1988; 1: 281–299.Google Scholar
10. Mendoza, M, Meugnier, HM, Etienne, J, Freney, J. Identification of Staphylococcus species by 16S-23S rDNA intergenic spacer PCR analysis. Int J Syst Bacteriol 1998; 48:1049–1055.Google Scholar
11. National Committee for Clinical Laboratory Standards (NCCLS). Performance Standards for Antimicrobial Susceptibility Testing. 12th suppl. M100-S12. Vilanova, PA: NCCLS; 2002.Google Scholar
12. Martineau, F, Picard, FJ, Danbing, KE, et al. Development of a PCR assay for identification of staphylococci at genus and species level. Clin Microbiol 2001;39:2541–2547.Google Scholar
13. Matar, GM, Chaar, MH, Araj, GF, Srour, Z, Jamaleddine, G, Hadi, U. Detection of a highly prevalent and potentially virulent strain of Pseudomonas aeruginosa from nosocomial infections in a medical center. BMC Microbiol 2005; 5:29.CrossRefGoogle ScholarPubMed