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Genotyping by Restriction Endonuclease Analysis Compared to Phenotyping by Antibiogram for Typing Methicillin-Resistant Staphylococcus aureus Strains Colonizing Patients in a Nursing Home

Published online by Cambridge University Press:  02 January 2015

Yee-Lean Lee  and
Lauri Thrupp
Yee-Lean Lee
Affiliation:
Infectious Disease Division, Department of Medicine, University of California Irvine, California
Lauri Thrupp*
Affiliation:
Infectious Disease Division, Department of Medicine, University of California Irvine, California
*
Chief Infection Control, Department of Medicine, UCI Medical Center, 101 City Dr, Orange, CA 92868
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Abstract

Objectives:

To assist in defining patterns of methicillin-resistant Staphylococcus aureus (MRSA) colonization in a skilled nursing facility (SNF), we compared genotyping by field-inversion gel electrophoresis (FIGE) restriction endonuclease digestion analysis (REA) with phenotyping by antibiogram for defining strain relatedness among MRSA isolates from SNF patients.

Design:

Prospective screening culture surveillance for MRSA among patients in a community SNE

Methods:

Nares and stool swab cultures were obtained from newly admitted patients and from all patients quarterly. MRSA were isolated by oxacillin screening agar. Antibiograms were determined by the disk-diffusion method, and genotyping was by FIGE REA.

Results:

It was shown that, among isolates with the same genotypes, many had different antibiograms; among isolates with the same antibiograms, many had different genotypes; and the discriminatory indices for isolates of MRSA by FIGE REA and by antibiogram were 0.56 and 0.78, respectively.

Conclusion:

Our study demonstrated that, in patients from one SNF, genotyping by FIGE REA identified two prevalent REA DNA types, but with variability of antibiogram patterns within each DNA type; the antibiogram also identified prevalent patterns with variability of REA DNA type within each antibiogram pattern. The discriminatory index of antibiograms alone, or of genotypes alone as determined by FIGE REA, was poor for strains of MRSA isolated from the SNF patients in our study.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 21 , Issue 3 , March 2000 , pp. 218 - 221
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2000

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