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Fungal Contamination in Hospital Environments

Published online by Cambridge University Press:  21 June 2016

F. Perdelli ,
M. L. Cristina ,
M. Sartini ,
A. M. Spagnolo ,
M. Dallera ,
G. Ottria ,
R. Lombardi ,
M. Grimaldi  and
P. Orlando
F. Perdelli*
Affiliation:
Department of Health Sciences, University of Genoa, Italy
M. L. Cristina
Affiliation:
Department of Health Sciences, University of Genoa, Italy
M. Sartini
Affiliation:
Department of Health Sciences, University of Genoa, Italy
A. M. Spagnolo
Affiliation:
Department of Health Sciences, University of Genoa, Italy
M. Dallera
Affiliation:
Department of Health Sciences, University of Genoa, Italy
G. Ottria
Affiliation:
Department of Health Sciences, University of Genoa, Italy
R. Lombardi
Affiliation:
Italian National Institute for Occupational Safety and Prevention (ISPESL), Rome, Italy
M. Grimaldi
Affiliation:
National Institute for Cancer Research (1ST), Genoa, Italy
P. Orlando
Affiliation:
Department of Health Sciences, University of Genoa, Italy
*
Department of Health Sciences, University of Genoa, Via Pastore, 1-16132 Genova, Italy ([email protected])
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Abstract

Objectives.

To assess the degree of fungal contamination in hospital environments and to evaluate the ability of air conditioning systems to reduce such contamination.

Methods.

We monitored airborne microbial concentrations in various environments in 10 hospitals equipped with air conditioning. Sampling was performed with a portable Surface Air System impactor with replicate organism detection and counting plates containing a fungus-selective medium. The total fungal concentration was determined 72-120 hours after sampling. The genera most involved in infection were identified by macroscopic and microscopic observation.

Results.

The mean concentration of airborne fungi in the set of environments examined was 19 ± 19 colony-forming units (cfu) per cubic meter. Analysis of the fungal concentration in the different types of environments revealed different levels of contamination: the lowest mean values (12 ± 14 cfu/m3) were recorded in operating theaters, and the highest (45 ± 37 cfu/m3) were recorded in kitchens. Analyses revealed statistically significant differences between median values for the various environments. The fungal genus most commonly encountered was Penicillium, which, in kitchens, displayed the highest mean airborne concentration (8 ± 2.4 cfu/m3). The percentage (35%) of Aspergillus documented in the wards was higher than that in any of the other environments monitored.

Conclusions.

The fungal concentrations recorded in the present study are comparable to those recorded in other studies conducted in hospital environments and are considerably lower than those seen in other indoor environments that are not air conditioned. These findings demonstrate the effectiveness of air-handling systems in reducing fungal contamination.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 27 , Issue 1 , January 2006 , pp. 44 - 47
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2006

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References

1.Lajonchere, JP, Feuilhade de Chauvin, M. Contamination by aspergillosis: evaluation of preventive measures and monitoring of the environment [in French[. Pathol Biol (Paris) 1994;42:718–729Google ScholarPubMed
2.Faure, O, Fricker-Hidalgo, H, Lebeau, B, Mallaret, MR, Ambroise-Thomas, P, Grillot, R. Eight-year surveillance of environmental fungal contamination in hospital operating rooms and haematological units. J Hosp Infect 2002; 50:155–160.Google Scholar
3.Fox, BC, Chamberlin, L, Kulich, P, Rae, EJ, Webster, LR. Heavy contamination of operating room air by Penicillum species: identification of the source and attempts at decontamination. Am J Infect Control 1990; 18:300–306.Google Scholar
4.Boyd, RF. Malattie da funghi. In: Boyd, RF, ed. Microbiologia generale, ed 1. Palermo: Medical Books; 1987:801–818Google Scholar
5.Ampel, NM. Emerging disease issues and fungal pathogens associated with HIV infection. Emerg Infect Dis 1996; 2:109–116.Google Scholar
6.Sangeorzan, JA, Bradley, SF, He, X, et al. Epidemiology of oral candidiasis in HIV-infected patients: colonization, infection, treatment, and emergence of fluconazole resistance. Am J Med 1994; 97:339–346.CrossRefGoogle ScholarPubMed
7.Powderly, WG, Robinson, K, Keath, EJ. Molecular epidemiology of recurrent oral candidiasis in human immunodeficiency virus-positive patients: evidence for two patterns of recurrence. J Infect Dis 1993; 168:463–466.Google Scholar
8.Martinez-Marcos, F, Cisneros, J, Gentil, M, et al. Prospective study of renal transplant infections in 50 consecutive patients. Eur J Clin Microbiol Infect Dis 1994; 13:1023–1028.Google Scholar
9.Grossi, P, Farina, C, Fiocchi, R, Dalla Gasperina, D. Prevalence and outcome of invasive fungal infections in 1963 thoracic organ transplant recipients: a multicenter retrospective study. Italian Study Group of Fungal Infections in Thoracic Organ Transplant Recipients. Transplantation 2000;70:112–116.Google Scholar
10.Kontoyiannis, DP, Bodey, GP. Invasive aspergillosis in 2002: an update. Eur J Clin Microbiol Infect Dis 2002; 21:161–172.Google Scholar
11.Cross, S. Mould spores: the unusual suspects in hay fever. Community Nurse 1997; 3:25–26.Google Scholar
12.Kurup, VP, Grunig, G. Animal models of allergic bronchopulmonary aspergillosis. Mycopathologia 2002; 153:165–177.Google Scholar
13.Kanny, G, Becker, S, de Hauteclocque, C, Moneret-Vautrin, DA. Airborne eczema due to mould allergy. Contact Dermatitis 1996; 35:378.Google Scholar
14.Morris, G, Kokki, MH, Anderson, K, Richardson, MD. Sampling of Aspergillus spores in air. J Hosp Infect 2000; 44:81–92.Google Scholar
15.Panagopoulou, P, Filioti, J, Petrikkos, G, et al. Environmental surveillance of filamentous fungi in three tertiary care hospitals in Greece. J Hosp Infect 2002; 52:185–191.Google Scholar
16.Rainer, J, Peintner, U, Poder, R. Biodiversity and concentration of airborne fungi in a hospital environment. Mycopathologia 2001; 149:87–97.CrossRefGoogle Scholar
17.Tormo Molina, R, Gonzalo Garijo, M, Munoz Rodriguez, AF, Silva Palacios, I. Pollen and spores in the air of a hospital out-patient ward. Allergol Immunopathol (Madr) 2002; 30:232–238.Google Scholar
18.Loudon, KW, Coke, AP, Burnie, JP, Shaw, AJ, Oppenheim, BA, Morris, CQ. Kitchens as a source of Aspergillus niger infection. J Hosp Infect 1996; 32:191–198.Google Scholar
19.Gorny, RL, Dutkiewicz, J. Bacterial and fungal aerosols in indoor environment in Central and Eastern European countries. Ann Agric Environ Med 2002; 9:17–23.Google Scholar
20.Dharmage, S, Bailey, M, Raven, J, et al. Prevalence and residential determinants of fungi within homes in Melbourne, Australia. Clin Exp Allergy 1999; 29:1481–1489.Google Scholar