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Evidence of Nosocomial Stenotrophomonas Maltophilia Cross-Infection in a Neonatology Unit Analyzed by Three Molecular Typing Methods

Published online by Cambridge University Press:  02 January 2015

Darío García de Viedma ,
Mercedes Marín ,
Emilia Cercenado ,
Roberto Alonso ,
Marta Rodríguez-Créixems  and
Emilio Bouza
Darío García de Viedma*
Affiliation:
Servicio de Microbiología y Enfermedades Infecciosas—HIV, Hospital General Universitario “Gregorio Marañón, “ Madrid, Spain
Mercedes Marín
Affiliation:
Servicio de Microbiología y Enfermedades Infecciosas—HIV, Hospital General Universitario “Gregorio Marañón, “ Madrid, Spain
Emilia Cercenado
Affiliation:
Servicio de Microbiología y Enfermedades Infecciosas—HIV, Hospital General Universitario “Gregorio Marañón, “ Madrid, Spain
Roberto Alonso
Affiliation:
Servicio de Microbiología y Enfermedades Infecciosas—HIV, Hospital General Universitario “Gregorio Marañón, “ Madrid, Spain
Marta Rodríguez-Créixems
Affiliation:
Servicio de Microbiología y Enfermedades Infecciosas—HIV, Hospital General Universitario “Gregorio Marañón, “ Madrid, Spain
Emilio Bouza
Affiliation:
Servicio de Microbiología y Enfermedades Infecciosas—HIV, Hospital General Universitario “Gregorio Marañón, “ Madrid, Spain
*
Servicio de Microbiología y Enfermedades Infecciosas—HIV, Hospital General Universitario “Gregorio Marañón, ” C/Dr. Esquerdo, 46. 28007 Madrid, Spain
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Abstract

Objective:

To characterize the epidemiological relationships among Stenotrophomonas maltophilia isolates in the neonatology unit of our institution over a 4-month period in which an increased number of isolates was observed.

Setting:

The neonatology ward in a 2,000-bed university hospital in Madrid, Spain.

Design:

A retrospective molecular epidemiological analysis using three different typing methods, arbitrarily primed polymerase chain reaction (PCR), pulsed-field gel electrophoresis, and enterobacterial repetitive intergenic consensus-PCR, was performed with 11 isolates obtained from seven neonates over a 4-month period. Presumed unrelated isolates also were included as controls. A similarity dendrogram was obtained, to analyze the genetic relatedness among the isolates.

Results:

All isolates from the neonates, except one, showed a remarkably high homology among their typing patterns for the three methods assayed and clustered in the relatedness dendrogram at 96% similarity. The unrelated strains selected as controls were unclustered. The index case was considered to be a newborn who had an S maltophilia isolate from a culture drawn on the day of admission to the neonatology unit and which was included in the clustered similarity group.

Conclusions:

Such a high genetic similarity among the isolates, together with the presence of an index case who had been colonized or infected by S maltophilia before arrival at our institution, constitutes the first evidence of nosocomial cross-transmission of this microorganism.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 20 , Issue 12 , December 1999 , pp. 816 - 820
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1999

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References

1. Denton, M, Todd, NJ, Kerr, KG, Hawkey, PM, Littlewood, JM. Molecular epidemiology of Stenotrophomonas maltophilia isolated from clinical specimens from patients with cystic fibrosis and associated environmental samples. J Clin Microbiol 1998;36:1953–1958.CrossRefGoogle ScholarPubMed
2. Fabe, C, Rodriguez, P, Cony Makhoul, P, Parneix, P, Bebear, C, Maugein, J. Molecular typing by pulsed field gel electrophoresis of Stenotrophomonas maltophilia isolated in a department of hematology. Pathol Biol (Paris) 1996;44:435–441.Google Scholar
3. Marty, N. Epidemiological typing of Stenotrophomonas maltophilia. J Hosp Infect 1997;36:261–266.Google Scholar
4. Vu-Thien, H, Moissenet, D, Valcin, M, Dulot, C, Tournier, G, Garbarg-Chenon, A. Molecular epidemiology of Burkholderia cepacia, Stenotrophomonas maltophilia and Alcaligenes xylosoxidans in a cystic fibrosis center. Eur J Clin Microbiol Infect Dis 1996;15:867–869.Google Scholar
5. Penzak, SR, Abate, BJ. Stenotrophomonas (Xanthomonas) maltophilia: a multidrug-resistant nosocomial pathogen. Pharmacotherapy 1997;17:293–301.Google Scholar
6. Davin-Regli, A, Bollet, C, Auffray, JP, Saux, P, De Micco, P. Use of random amplified polymorphic DNA for epidemiological typing of Stenotrophomonas maltophilia. J Hosp Infect 1996;32:39–50.Google Scholar
7. Felicia, P, Laing, Y, Ramotar, K, Read, RR, Alfieri, N, Kureishi, A, et al. Molecular epidemiology of Xanthomonas maltophilia colonization and infection in the hospital environment. J Clin Microbiol 1995;33:513–518.Google Scholar
8. Gerner-Smidt, P, Bruun, B, Arpi, M, Schmidt, J. Diversity of nosocomial Xanthomonas maltophilia (Stenotrophomonas maltophilia) as determined by ribotyping. Eur J Clin Microbiol Infert Dis 1995;14:137–140.Google Scholar
9. Yao, JD, Conly, JM, Krajden, M. Molecular typing of Stenotrophomonas (Xanthomonas) maltophilia by DNA macrorestriction analysis and random amplified polymorphic DNA analysis. J Clin Microbiol 1995;33:2195–2198.Google Scholar
10. Cornwell, EE 3d, Willey, P, Belzberg, H, Berne, TV, Morales, IR, Demetriades, D. Characteristics of Xanthomonas infections in critically ill surgical patients. Am Surg 1996;62:478–480.Google Scholar
11. Denton, M, Kerr, KG. Microbiological and clinical aspects of infection associated with Stenotrophomonas maltophilia. Clin Microbiol Rev 1998;11:57–80.CrossRefGoogle ScholarPubMed
12. Garrison, MW, Anderson, DE, Campbell, DM, Carroll, KC, Malone, CL, Anderson, JD, et al. Stenotrophomonas maltophilia: emergence of multidrug-resistant strains during therapy and in an in vitro pharmacodynamic chamber model. Antimicrob Agents Chemother 1996;40:2859–2864.Google Scholar
13. Chatelut, M, Dournes, JL, Chabanon, G, Marty, N. Epidemiological typing of Stenotrophomonas (Xanthomonas) maltophilia by PCR. J Clin Microbiol 1995;33:912–914.CrossRefGoogle ScholarPubMed