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A vector for studying plasmid stability functions in Streptomyces

Published online by Cambridge University Press:  14 April 2009

Kevin Kendall  and
John Cullum
Kevin Kendall
Affiliation:
Department of Biochemistry and Applied Molecular BiologyUniversity of Manchester Institute of Science and TechnologyP. O. Box 88 Manchester M60 1QD, U.K.
John Cullum*
Affiliation:
Department of Biochemistry and Applied Molecular BiologyUniversity of Manchester Institute of Science and TechnologyP. O. Box 88 Manchester M60 1QD, U.K.
*
† Correponding author
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Summary

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We constructed a cloning vector (pMT603) based on the low copy number plasmid SCP2*. pMT6O3 is unstable because it lacks the SCP2* stability region and carries the selectable marker thiostrepton-resistance and a tyrosinase gene which results in melanin production. This allows easy testing of plasmid stability and we demonstrated its usefulness by cloning a plasmid stability function.

Type
Short Paper
Information
Genetics Research , Volume 51 , Issue 1 , February 1988 , pp. 71 - 74
Copyright
Copyright © Cambridge University Press 1988

References

Bibb, M. J. & Hopwood, D. A. (1981). Genetic studies of the fertility plasmid SCP2 and its SCP2* variants in Streptomyces coelicolor A3(2). Journal of General Microbiology 126, 427–442.Google Scholar
Bibb, M., Schottel, J. L. & Cohen, S. N. (1980). A DNA cloning system for interspecies gene transfer in antibiotic-producing Streptomyces. Nature 284, 526–531.Google Scholar
Hopwood, D. A., Kieser, T., Wright, H. M. & Bibb, M. J. (1983). Plasmids, recombination and chromosome mapping in Streptomyces lividans 66. Journal of General Microbiology 129, 2257–2269.Google ScholarPubMed
Katz, E., Thompson, C. J. & Hopwood, D. A. (1983). Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans. Journal of General Microbiology 129, 2703–2714.Google Scholar
Kendall, K., Ali-Dunkrah, U. & Cullum, J. (1987). Cloning of the galactokinase gene (gal K) from Streptomyces coelicolor A3(2). Journal of General Microbiology 133, 721–725.Google Scholar
Kendall, K. & Cullum, J. (1984). Cloning and expression of an extracellular agarase gene from Streptomyces coelicolor A3(2) in Streptomyces lividans 66. Gene 29, 315–321.Google Scholar
Kieser, T., Hopwood, D. A., Wright, H. M. & Thompson, C. J. (1982). pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors. Molecular and General Genetics 185, 223–238.CrossRefGoogle ScholarPubMed
Larson, J. L. & Hershberger, C. L. (1986). The minimal replicon of a Streptomycete plasmid produces an ultrahigh level of plasmid DNA. Plasmid 15, 199–209.CrossRefGoogle ScholarPubMed
Lydiate, D. J., Malpartida, F. & Hopwood, D. A. (1985). The Streptomyces plasmid SCP2*: its functional analysis and development into useful cloning vectors. Gene 35, 223–235.CrossRefGoogle ScholarPubMed
Meacock, P. A. & Cohen, S. N. (1980). Partitioning of bacterial plasmids during cell division: a cis-acting locus that accomplishes stable plasmid inheritance. Cell 20, 529–542.Google Scholar
Summers, D. K. & Sherratt, D. J. (1984). Multimerization of high copy number plasmids causes instability: ColEl encodes a determinant essential for plasmid monomerization and stability. Cell 36, 1097–1103.CrossRefGoogle Scholar