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Variation over space and time of Aedes aegypti in Phnom Penh (Cambodia): genetic structure and oral susceptibility to a dengue virus

Published online by Cambridge University Press:  24 February 2004

CHRISTOPHE PAUPY ,
NGAN CHANTHA ,
MARIE VAZEILLE ,
JEAN-MARC REYNES ,
FRANCOIS RODHAIN  and
ANNA-BELLA FAILLOUX
CHRISTOPHE PAUPY
Affiliation:
Unité des Insectes et Maladies Infectieuses, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France Present address: Laboratoire de Microbiologie et Environnement, Institut Pasteur de La Nouvelle Calédonie, BP 61, 98845 Nouméa, New Caledonia.
NGAN CHANTHA
Affiliation:
National Malaria Centre, 372 boulevard Monivong, Phnom Penh, Cambodia
MARIE VAZEILLE
Affiliation:
Unité des Insectes et Maladies Infectieuses, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
JEAN-MARC REYNES
Affiliation:
Unité de Virologie, Institut Pasteur du Cambodge, 5 boulevard Monivong, Phnom Penh, Cambodia
FRANCOIS RODHAIN
Affiliation:
Ecole Pasteurienne d'Infectiologie, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
ANNA-BELLA FAILLOUX
Affiliation:
Unité des Insectes et Maladies Infectieuses, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
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Abstract

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We studied spatial and temporal variation in 20–23 Aedes aegypti samples collected in Phnom Penh and its suburbs to estimate the population genetic structure using allozymes and the susceptibility to a dengue-2 virus. Based on seven allozyme systems, we detected low levels of genetic exchanges (i.e. high, significant FST values) between populations collected in the city centre, and different patterns of genetic structure for samples collected in the suburbs, depending on the type of environment and the date of collection. In the southern suburbs and the Chroy Chang Var Peninsula, differentiation became highly significant at the end of the dry season, whereas the opposite situation was observed for collections from the northern suburbs. Vector competence assessed by oral infections with a dengue-2 virus was lower for samples collected in the city centre than in the suburbs. A significant decrease of dengue susceptibility was observed in populations during the dry season. This study allows a model of Ae. aegypti population functioning in Phnom Penh to be suggested. Dynamics of dengue virus diffusion depend on the population genetic structure of the vector and its evolution over space and time.

Type
Research Article
Information
Genetics Research , Volume 82 , Issue 3 , December 2003 , pp. 171 - 182
Copyright
© 2003 Cambridge University Press