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Valine-resistant mutants of Escherichia coli K-12

Published online by Cambridge University Press:  14 April 2009

S. W. Glover
S. W. Glover
Affiliation:
M.R.C. Microbial Genetics Research Unit, Hammersmith Hospital, London, W.12
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Sixty-two mutants of E. coli K-12 resistant to 40 μg./ml. valine were isolated from a sensitive strain. Transduction experiments using phage P1 showed that one group of these mutants, val-r-C, is closely linked to leu, another group, val-r-B is closely linked to thr, and a third mutant, val-r-D57, lies between leu and thr. Conjugation experiments showed that the remainder of the mutants could be divided into three groups, val-r-A, val-r-E and val-r-F on the basis of their different times of transfer from Vhf donors to val-s F− recipients. All the mutants are sensitive to 10,000 μg./ml. valine; val-r-B and val-r-D mutants are resistant up to 80 μg./ml. valine; val-r-C mutants are resistant up to 1000 μg./ml. valine, and val-r-A, val-r-E and val-r-F mutants up to 5000 μg./ml. The functional significance of the genetic locations of these groups is discussed.

Type
Research Article
Information
Genetics Research , Volume 3 , Issue 3 , November 1962 , pp. 448 - 460
Copyright
Copyright © Cambridge University Press 1962

References

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