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Use of isozymes as chromosome markers in wheat-rye addition lines and in triticale*

Published online by Cambridge University Press:  14 April 2009

K. S. Tang  and
G. E. Hart
K. S. Tang
Affiliation:
Genetics Section, Department of Plant Sciences, Texas A & M University, College Station, Texas 77843
G. E. Hart
Affiliation:
Genetics Section, Department of Plant Sciences, Texas A & M University, College Station, Texas 77843
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Summary

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The alcohol dehydrogenase (ADH), glutamate oxaloacetate transaminase (GOT), acid phosphatase (ACPH), endopeptidase (EP) and aminopeptidase (AMP) zymogram phenotypes of Chinese Spring wheat, Imperial rye, the Chinese Spring-Imperial triticale and the series of seven disomic Imperial chromosome additions to Chinese Spring were determined. It was found that the zymogram phenotypes produced for one or more of the enzymes by each of the Imperial chromosomes 3, 6, C and D differ sufficiently from that of Chinese Spring so as to provide evidence for the presence or absence of each of these chromosomes in addition lines and triticales. The structural genes Got-R2 and Got-R3 were located in Imperial chromosomes 6 and 3 respectively and other genes involved in the production of GOT in chromosomes C and D. By analysis of GOT alone, evidence for the presence or absence of Imperial chromosomes 3, 6, C and D in addition lines and triticales can be obtained. Adh-R1 was located in chromosome C and a gene(s) involved in the production of an ACPH was located in chromosome D.

The linkages obtained for Got-R2, Got-R3 and Adh-R1 demonstrate homoeology between the Imperial chromosomes 3, 6 and C and the Chinese Spring chromosomes of groups 3, 6 and 4 respectively. The discovery that Adh-R1 is located in Imperial chromosome C also suggests that the 4R/7R and 7R/4R homoeologous groupings proposed elsewhere for the chromosomes of the rye cultivars Imperial, Dakold, and King II should be reassessed, since they are inconsistent with the known linkages of Adh-R1 in the three cultivars. The finding in King II of two forms of ADH and of two ADH genes has been reported. The results of our study of Imperial, King II, and Dakold indicate that rye possesses but one ADH and only one ADH structural gene.

Type
Research Article
Information
Genetics Research , Volume 26 , Issue 2 , October 1975 , pp. 187 - 201
Copyright
Copyright © Cambridge University Press 1975

References

REFERENCES

Barber, H. N., Driscoll, C. J., Long, P. M. & Vickery, R. S. (1968). Protein genetics of wheat and homoeologous relationships of chromosomes. Nature 218, 450–452.CrossRefGoogle Scholar
Bergman, J. W. & Maan, S. S. (1973). Genetic control of isozymes in wheat-rye addition lines with rye or wheat cytoplasm. Proceedings of the Fourth International Wheat Genetics Symposium, pp. 329–336.Google Scholar
Carlson, P. S. (1972). Locating genetic loci with aneuploids. Molecular & General Genetics 114, 273–280.Google Scholar
Darvey, N. L. (1973). Genetics of seed shrivelling in wheat and triticale. Proceedings of the Fourth International Wheat Genetics Symposium, pp. 155–160.Google Scholar
Darvey, N. L. & Gustafson, J. P. (1975). Identification of rye chromosomes in wheat-rye addition lines and triticale by means of heterochromatin bands. Crop Science 15, 239–243.CrossRefGoogle Scholar
Gill, B. S. & Kimber, G. (1974 a). A Giemsa C-banding technique for cereal chromosomes. Cereal Research Communications 2, 87–94.Google Scholar
Gill, B. S. & Kimber, G. (1974 b). A Giemsa C-banded karyotype of rye. Proceedings of the National Academy of Sciences, U.S.A. 71, 1247–1249.CrossRefGoogle ScholarPubMed
Hart, G. E. (1970). Evidence for triplicate genes for alcohol dehydrogenase in hexaploid wheat. Proceedings of the National Academy of Sciences, U.S.A. 66, 1136–1141.Google Scholar
Hart, G. E. (1973). Homoeologous gene evolution in hexaploid wheat. Proceedings of the Fourth International Wheat Genetics Symposium, pp. 805–810.Google Scholar
Hart, G. E. (1975). Glutamate oxaloacetate transaminase isozymes of Triticum: Evidence for multiple systems of triplicate structural genes in hexaploid wheat. In Isozymes. Vol. iii. Developmental Biology (ed. Markert, C. L.), pp. 637–657. Academic Press.Google Scholar
Hart, G. E. & Langston, P. (1975). Evidence for triplicate endopeptidase and lipoxygenase structural gene sets in hexaploid wheat. Isozyme Bulletin 8, 12.Google Scholar
Irani, B. X. & Bhatia, C. R. (1972). Chromosomal location of alcohol dehydrogenase gene(s) in rye, using wheat-rye addition lines. Genetica 43, 195–200.Google Scholar
Nishikawa, K. & Nobuhara, M. (1971). Genetic studies of α-amylase isozymes in wheat. I. Location of genes and variation in tetra- and hexaploid wheat. Japanese Journal of Genetics 46, 345–353.Google Scholar