Hostname: page-component-6bf8c574d5-956mj Total loading time: 0 Render date: 2025-02-17T16:40:51.468Z Has data issue: false hasContentIssue false
  • English
  • Français

Unstable drug resistance in Staphylococcus aureus M4

Published online by Cambridge University Press:  14 April 2009

W. B. Grubb  and
D. I. Annear
W. B. Grubb
Affiliation:
Biological Laboratories, University of Kent, Canterbury, England, Department of Microbiology, University of Western Australia
D. I. Annear
Affiliation:
Microbiology Department, Royal Perth Hospital, Perth, Western Australia
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Staphylococcus aureus M4 has chromosomal resistance to streptomycin, plasmid-borne resistance to penicillin and tetracycline and probably chromosomal inducible erythromycin resistance. It also has constitutive erythromycin resistance which is unstable and linked to kanamycin and lincomycin resistance and the ability to produce a diffusible pigment. Variants have been isolated which have stable kanamycin, lincomycin and constitutive erythromycin resistance and these do not produce the diffusible pigment. In transduction experiments kanamycin, lincomycin and constitutive erythromycin resistance were always co-transduced together with streptomycin resistance. The transduction frequencies were approximately 100 times higher with the stable variants compared with the parent. The transductants, irrespective of the donor used, all had stable resistance and did not produce the diffusible pigment. Although transduction with UV-irradiated transducing lysates was characteristic of a plasmid, no corresponding plasmid DNA has been detected.

Type
Research Article
Information
Genetics Research , Volume 38 , Issue 3 , December 1981 , pp. 217 - 223
Copyright
Copyright © Cambridge University Press 1981

References

REFERENCES

Annear, D. I. & Grubb, W. B. (1969 a). Spontaneous loss of resistance to kanamycin and other antibiotics in methicillin-resistant cultures of Staphylococcus aureus. Medical Journal of Australia 2, 902–904.CrossRefGoogle Scholar
Annear, D. I. & Grubb, W. B. (1969 b). Pigmentation of medium by cultures of Staphylococcue aureus. Medical Journal of Australia 2, 1107.CrossRefGoogle ScholarPubMed
Annear, D. I. & Grubb, W. B. (1972). Unstable resistance to kanamycin, lincomycin and penicillin in a methicillin resistant culture of Staphylococcus aureus. Pathology 4, 247–252.CrossRefGoogle Scholar
Arber, W. (1960). Transduction of chromosomal genes and episomes in Escherichia coli. Virology 11, 273–288.CrossRefGoogle ScholarPubMed
Blair, J. E. & Williams, R. E. O. (1961). Phage typing of staphylococci. Bulletin of the World Health Organization 24, 771–784.Google ScholarPubMed
Bouanchaud, D. H., Scavizzi, M. R. & Chabbert, Y. A. (1969). Elimination by ethidium bromide of antibiotic resistance in enterobacteria and staphylococci. Journal of General Microbiology 54, 417–425.CrossRefGoogle Scholar
Grinsted, J. & Lacey, R. W. (1973). Genetic variation of streptomycin resistance in clinical strains of Staphylococcua aureus. Journal of Medical Microbiology 6, 351–361.CrossRefGoogle ScholarPubMed
Grubb, W. B. & O'Reilly, R. J. (1971). Joint transduction of separate extrachromosomal drug resistance determinants in Staphylococcus aureus E169. Biochemical and Biophysical Research Communications 42, 377–383.CrossRefGoogle ScholarPubMed
Grubb, W. B., O'Reilly, R. J. & May, J. W. (1972). Segregation of co-transduced streptomycin and tetracycline resistance in Staphylococcus aureus. Genetical Research 20, 43–50.CrossRefGoogle ScholarPubMed
Hughes, C. & Meynell, G. G. (1977). Rapid screening for plasmid DNA. Molecular and General Genetics 151, 175–179.CrossRefGoogle ScholarPubMed
Lacey, R. W. & Chopra, I. (1974). Genetic studies of a multi-resistant strain of Staphylococcus aureus. Journal of Medical Microbiology 7, 285–297.CrossRefGoogle ScholarPubMed
May, J. W., Houghton, R. H. & Perret, C. J. (1964). The effect of growth at elevated temperatures on some heritable properties of Staphylococcus aureus. Journal of General Microbiology 37, 157–169.CrossRefGoogle ScholarPubMed
Novick, R. P. & Bouanchaud, D. (1971). Extrachromosomal nature of drug resistance in Staphylococcus aureus. Annals of the New York Academy of Sciences 182, 279–294.CrossRefGoogle ScholarPubMed
Novick, R. P., Edelman, I., Schwesinger, M. D., Gruss, A. D., Swanson, E. C. & Pattee, P. A. (1979). Genetic translocation in Staphylococcus aureus. Proceedings of the National Academy of Sciences of the U.S.A. 76, 400–404.CrossRefGoogle ScholarPubMed
Pariza, M. W. & Iandolo, J. J. (1974). Determination of genome size of selected typing bacteriophages of Staphylococcus aureus. Applied Microbiology 28, 510–512.CrossRefGoogle ScholarPubMed
Pattee, P. A. & Baldwin, J. N. (1962). Transduction of resistance to some macrolide antibiotics in Staphylococcus aureus. Journal of Bacteriology 84, 1049–1055.CrossRefGoogle ScholarPubMed
Phillips, S. & Novick, R. P. (1979). Tn 554 – a site-specific represser-controlled transposon in Staphylococcus aureus. Nature 278, 476–478.CrossRefGoogle Scholar
Richmond, M. H. (1972). Plasmids and extrachromosomal genetics in Staphylococcus aureus. In The Staphylococci (ed. Cohen, J. O.), pp. 159–186. New York, London, Sydney, Toronto: Wiley-Interscience.Google Scholar
Richmond, M. H. & Johnston, J. (1969). The reversible transition of certain genes in Staphylococcus aureus between the integrated and the extra-chromosomal state. Genetical Research 13, 267–274.CrossRefGoogle ScholarPubMed
Rubin, S. J. & Rosenblum, E. D. (1971). Effects of the recipient strain and ultraviolet irradiation on transduction kinetics of the penicillinase plasmid of Staphylococcus aureus. Journal of Bacteriology 108, 1192–1199.CrossRefGoogle ScholarPubMed
Weisblum, B., Siddhikol, C., Lai, C. J. & Demohn, V. (1971). Erythromycin-inducible resistance in Staphylococcus aureus: requirements for induction. Journal of Bacteriology 106, 835–847.CrossRefGoogle ScholarPubMed