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The two-way selection of mutants and revertants in respect of acetate utilization and resistance to fluoro-acetate in Aspergillus nidulans

Published online by Cambridge University Press:  14 April 2009

David Apirion
David Apirion
Affiliation:
Department of Genetics, The University, Glasgow*
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An extension of two-way selection (i.e. selection of mutant from wild-type and vice versa within the same locus and with the same efficiency) to four different mutational or segregational situations was made possible by using acetate, fluoro-acetate and other substances related to their metabolism.

Two types of mutants resistant to fluoro-acetate were selected, the first of which (designated fac) cannot grow on acetate as the sole carbon source, while the second (designated fan) can.

Commencing with either a fac or a fan strain a double fac fan strain may be isolated, which is much more resistant to fluoro-acetate than either single mutant strain. Such double mutant strains may also be obtained by crossing a fac to a fan strain. Various characteristics of growth response of these strains on various media were observed.

fac mutants are recessive and map in three meiotically unlinked loci, one in linkage group V and two in linkage group VIII.

fan mutants are recessive and map in five loci, one in each of the linkage groups V, VII and VIII, and two linked in linkage group VI.

Most fac mutants isolated did not revert and this failure is considered genuine. Of the revertants tested, most resulted from extra-cistron suppressors, while revertants of two fac mutants resulted from very closely linked or intra-cistron suppressors.

It is argued that the findings indicate the existence of two pathways for acetate utilization in Aspergillus nidulans, a major and a minor; fac mutants are blocked in the major pathway, fan mutants in the minor pathway.

Type
Research Article
Information
Genetics Research , Volume 6 , Issue 3 , November 1965 , pp. 317 - 329
Copyright
Copyright © Cambridge University Press 1965

References

REFERENCES

Apirion, D. (1962). A general system for the automatic selection of auxotrophs from prototrophs and vice versa in micro-organisms. Nature, Lond. 195, 959–961.CrossRefGoogle Scholar
Apirion, D. (1963). Ph.D. Thesis, Glasgow University.Google Scholar
Aspergillus News Letter (1963). Glasgow list of located, or partially located, mutants of Aspergillus nidulans. No. 4, pp. 12–14.Google Scholar
Forbes, E. (1959). Use of mitotic segregation for assigning genes to linkage groups in Aspergillus nidulans. Heredity, Lond. 13, 67–80.CrossRefGoogle Scholar
Forbes, E. (1963). A strain with all chromosomes marked for use in haploidization. Aspergillus News Letter, 4, 15.Google Scholar
Grigg, G. W. (1952). Back mutation assay method in micro-organisms. Nature, Lond. 169, 98–100.CrossRefGoogle ScholarPubMed
Käfer, E. (1958). An 8-chromosome map of Aspergillus nidulans. Adv. Genet. 9, 105–145.Google Scholar
Kornberg, H. L. & Elsden, S. R. (1961). The metabolism of C2 compounds by microorganisms. Adv. Enzymol. 23, 401–470.Google Scholar
Lhoas, P. (1961). Mitotic haploidization by treatment of Aspergillus niger diploids with para-fluorophenylalanine. Nature, Lond. 190, 744.CrossRefGoogle ScholarPubMed
Morpurgo, G. (1961). Somatic segregation induced by p-fluorophenylalanine (pfp). Aspergillus News Letter, 2, 10.Google Scholar
Pontecorvo, G. (1949). Auxanographic techniques in biochemical genetics. J. gen. Microbiol. 3, 122–126.Google Scholar
Pontecorvo, G. (1963). Microbial genetics: retrospect and prospect. Proc. R. Soc. B, 158, 1.Google Scholar
Pontecorvo, G. & Käfer, E. (1956). Mapping the chromosome by means of mitotic recombination. Proc. R. phys. Soc. Edinb. 25, 16–20.Google Scholar
Pontecorvo, G. & Käfer, E. (1958). Genetic analysis based on mitotic recombination. Adv. Genet. 9, 71–104.CrossRefGoogle ScholarPubMed
Pontecorvo, G., Roper, J. A., Hemmons, L. M., Macdonald, K. D. & Bufton, A. W. J. (1953). The genetics of Aspergillus nidulans. Adv. Genet. 5, 141–238.Google Scholar
Pontecorvo, G., Tarr Gloor, E. & Forbes, E. (1954). Analysis of mitotic recombination in Aspergillus nidulans. J. Genet. 52, 226–237.CrossRefGoogle Scholar
Roper, J. A. (1952). Production of heterozygous diploids in filamentous fungi. Experientia, 8, 14–15.CrossRefGoogle ScholarPubMed
Siddiqi, O. H. (1962). Mutagenic action of nitrous acid on Aspergillus nidulans. Genet. Res. 3, 303–314.CrossRefGoogle Scholar
Vanderwinkel, E., Liard, P., Ramos, F. & Wiame, J. M. (1963). Genetic control of the regulation of isocitratase and malate synthase in Escherichia coli K12. Biochem. biophys. Res. Commun. 12, 157–162.CrossRefGoogle Scholar