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Two mutations giving low-level streptomycin resistance in Escherichia coli K12

Published online by Cambridge University Press:  14 April 2009

Lilian M. Roberts  and
E. C. R. Reeve
Lilian M. Roberts
Affiliation:
Institute of Animal Genetics, West Mains Road, Edinburgh 9
E. C. R. Reeve
Affiliation:
Institute of Animal Genetics, West Mains Road, Edinburgh 9

Summary

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A mutation in Escherichia coli K12 giving resistance to about 5 μg/ml of streptomycin was found to be cotransducible by P1 with proA and proB, and is located at about 8·5 min on the chromosome map. The locus is named strB. A second mutation to the same resistance level was not cotransducible with either proA or proB and must be located elsewhere. Both mutations cause a marked increase in R-factor mediated streptomycin resistance, and significant decreases in resistance to several other antibiotics, both in the presence and absence of an R-factor determinant for the same antibiotic. The two mutations differ in their effects on bacterial sensitivity to crystal violet and EDTA.

Type
Short Papers
Information
Genetics Research , Volume 16 , Issue 3 , December 1970 , pp. 359 - 365
Copyright
Copyright © Cambridge University Press 1970

References

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