Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-11-28T00:44:25.638Z Has data issue: false hasContentIssue false
  • English
  • Français

Two isoforms of the kidney androgen-regulated protein are encoded by two alleles of a single gene in OFl mice

Published online by Cambridge University Press:  14 April 2009

E. Melanitou ,
D. Tronik  and
F. Rougeon
E. Melanitou
Affiliation:
Institut Pasteur, Unité de Génétique et Biochimie du Développement, Département d'Immunologie, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
D. Tronik
Affiliation:
Institut Pasteur, Unité de Génétique et Biochimie du Développement, Département d'Immunologie, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
F. Rougeon*
Affiliation:
Institut Pasteur, Unité de Génétique et Biochimie du Développement, Département d'Immunologie, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
*
* Corresponding author.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Two cDNA clones coding for two forms of the mouse kidney androgen-regulated protein (KAP) distinguished by their electrophoretic mobilities on SDS gel electrophoresis have been isolated from libraries prepared from strains of mice having one (BALB/c) or two (OFl) forms of the KAP protein. The corresponding mRNAs have identical sizes, as well as identical sequences in their 5' non-translated regions. The size difference observed between the two proteins is due to two point mutations in the coding region of the KAP mRNA, leading to two amino-acid changes one of which resulted in the substitution of a glycine for a glutamic acid. As shown by in vitro transcription/translation experiments, these two amino-acid differences are responsible for the shift in the apparent molecular weight of the protein on SDS gels. Both forms of the protein are more abundant in males than in females.

In vitro translation of kidney RNAs isolated from six different strains and species of mice revealed the presence of other forms of the KAP protein, characterized by small variations of their molecular weights. Southern blot analysis data are consistent with the presence of only one kap gene in the mouse genome. A restriction fragment length polymorphism has been observed, which does not correlate with the protein polymorphism, indicating the presence of another allele in the OF1 mouse genome.

Type
Research Article
Information
Genetics Research , Volume 59 , Issue 2 , April 1992 , pp. 117 - 124
Copyright
Copyright © Cambridge University Press 1992

References

Auffray, C. & Rougeon, F. (1980). Purification of mouse immunoglobulin heavy chain messenger RNAs from total myeloma tumor RNA. European Journal of Biochemistry 107, 303–314.CrossRefGoogle ScholarPubMed
Berger, F. G. & Bauman, H. (1985). An evolutionary switch in tissue-specific gene expression: abundant expression of aj-antitrypsin in the kidney of a wild mouse species. Journal of Biological Chemistry 60, 1160–1165.CrossRefGoogle Scholar
Berger, F. G., Gross, K. W. & Watson, G. (1981). Isolation and characterization of a DNA sequence complementary to an androgen-inducible messenger RNA from mouse kidney. Journal of Biological Chemistry 56, 7006–7013.CrossRefGoogle Scholar
Bonhomme, F. (1986). Evolutionary relationships in the genus Mus. Current Topics in Microbiology and Immunology 17, 19–34.Google Scholar
Bonhomme, F., Catalan, J., Britton-Davidian, J., Chapman, V. M., Moriwaki, D., Nero, E. & Thaler, L. (1984). Biochemical diversity and evolution in the genus Mus. Biochemical Genetics 22, 275–303.CrossRefGoogle ScholarPubMed
Bonhomme, F. & Guenet, J. L. (1989). The wild-house mouse and its relatives in genetic variants and strains of the laboratory mouse (ed. Lyon, M. F. and Searle, A. G.), pp. 649–666. Oxford University Press.Google Scholar
Callahan, R. & Todaro, G. J. (1978). Four major endogenous retrovirus classes each genetically transmitted in various species. In Origins of Inbred Mice (ed. Morse, H. C. III), pp. 689–713. New York: Academic Press.CrossRefGoogle Scholar
Catterall, J. F., Kontula, K. K., Watson, C. S., Seppanen, P. J., Funkenstein, B., Melanitou, E., Hickok, N. J., Bardin, C. W. & Janne, O. A. (1986). Regulation of gene expression by androgens in murine kidney. Recent Progress in Hormone Research 4, 71–109.Google Scholar
Chapman, V. M., Paigen, K., Siracusa, L. & Womack, J. E. (1979). Biochemical variation: mouse. In Inbred and Genetically Defined Strains of Laboratory Animals (ed. Altman, P. L. and Katz, D. D.), pp. 77–95. Federation of the American Societies for Experimental Biology, Bethesda, Md.Google Scholar
Chupin-Rosinski, I., Tronik, D. & Rougeon, F. (1988). High level of accumulation of a mRNA coding for a precursor-like protein in the submaxillary gland of male rats. Proceedings of the National Academy of Sciences, USA 85, 8553–8557.CrossRefGoogle Scholar
Claverie, J. M. & Bricault, L. (1986). Proteins, structure and function. Genetics 1, 60–65.Google Scholar
Dickinson, D. P., Gross, K. W., Piccini, N. & Wilson, C. M. (1984). Evolution and variation of renin genes in mice. Genetics 108, 651–667.CrossRefGoogle ScholarPubMed
Dreyfus, M, Doyen, N. & Rougeon, F. (1987). The conserved decanucleotide from the immunoglobulin heavy chain promoter induces a very high transcriptional activity in B-cells when introduced into an heterologous promoter. EMBO Journal 6, 1685–1690.CrossRefGoogle ScholarPubMed
Elliot, R. W. & Berger, F. G. (1983). DNA sequence polymorphism in an androgen-regulated gene is associated with alteration in the encoded RNAs. Proceedings of the National Academy of Sciences, USA 80, 501–504.CrossRefGoogle Scholar
Feinberg, A. P. & Vogelstein, B. (1983). A technique for radiolabeling DNA restriction endonuclease fragment to high specific activity. Analytical Biochemistry 13, 6–13.CrossRefGoogle Scholar
Ferris, S. D., Sage, R. D., Prager, E. M., Ritte, U. & Wilson, A. C. (1983). Mitochondrial DNA evolution in mice. Genetics 105, 681–671.CrossRefGoogle ScholarPubMed
Hammer, M. F. & Wilson, A. C. (1987). Regulatory and structural genes for lysozymes of mice. Genetics 115, 51–533.CrossRefGoogle ScholarPubMed
King, D., Snider, L. D. & Lingrel, J. D. (1986). Polymorphism in an androgen-regulated mouse gene is the result of the insertion of a Bl repetitive element into the transcription unit. Molecular and Cellular Biology 6, 609–617.Google Scholar
Lipman, D. & Pearson, W. R. (1985). Rapid and sensitive protein similarity searches. Science 7, 1435–1440.CrossRefGoogle Scholar
Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.Google Scholar
Melanitou, E., Cohn, A. D., Bardin, C. W. & Janne, O. A. (1987). Genetic variation in androgen regulation of ornithine decarboxylase gene expression in inbred strains of mice. Molecular Endocrinology 1, 66–73.CrossRefGoogle ScholarPubMed
Melanitou, E., Simon, D., Guenet, J. L. & Rougeon, F. (1991). The gene coding for the ‘Kidney Androgenregulated protein’ (Kap), maps between the Gapd and Kras locus on mouse chromosome 6. Mammalian Genome 1, 191–19.CrossRefGoogle Scholar
Melton, D. A., Krieg, P. A., Rebagliati, M. R., Maniatis, T., Zinn, K. & Green, M. R. (1984). Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Research 1, 7035–7056.CrossRefGoogle Scholar
Meseguer, A., Watson, C. S. & Catterall, J. F. (1989). Nucleotide sequence of kidney androgen-regulated protein mRNA and its cell-specific expression in Thm/Y mice. Molecular Endocrinology 3, 96–967.CrossRefGoogle Scholar
Messing, J. & Vieira, J. (1982). A new pair of M13 vector for selecting either DNA strand of double-digest restriction fragments. Gene 19, 69–76.CrossRefGoogle ScholarPubMed
Mirels, L., Bedi, G. S., Dickinson, D. P., Gross, K. W. & Tabak, L. A. (1987). Molecular characterization of glutamic acid/glutamic-rich secretory proteins from rat submandibular glands. Journal of Biological Chemistry 6, 789–797.Google Scholar
Niu, E. M., Meseguer, A. & Catterall, J. F. (1991). Genomic organization and DNA sequence of the mouse Kidney Androgen-regulated Protein (KAP) gene. DNA and Cell Biology 1, 41–48.CrossRefGoogle Scholar
Panthier, J. J., Holm, I. & Rougeon, F. (1982). The mouse Rn locus: S allele of the renin regulator gene results from a single structural gene duplication. EM BO Journal 1, 1417–141.Google ScholarPubMed
Paterson, B. M., Roberts, B. E. & Kuff, E. L. (1977). Structural gene identification and mapping by DNAmRNA hybrid-arrested cell-free translation. Proceedings of the National Academy of Sciences USA 74, 4370–4374.CrossRefGoogle ScholarPubMed
Pelham, H. R. B. & Jackson, R. J. (1976). An efficient mRNA-dependent translation system from reticulocyte lysates. European Journal of Biochemistry 67, 47–56.CrossRefGoogle ScholarPubMed
Piccini, N., Knopf, J. L. & Gross, K. W. (1982). A DNA polymorphism, consistent with gene duplication, correlates with high renin levels in the mouse submaxillary gland. Cell 30, 5–13.CrossRefGoogle ScholarPubMed
Proodfoot, N. J. & Brownlee, G. G. (1976). 3’ non-coding regions sequences in eucaryotic messenger RNA. Nature (London) 63, 11–14.Google Scholar
Rougeon, F., Chambraud, B., Foote, S., Panthier, J. J., Nageotte, R. & Corvol, P. (1981). Molecular cloning of a mouse submaxillary gland renin cDNA fragment. Proceedings of the National Academy of Sciences USA 78, 6367–6371.CrossRefGoogle ScholarPubMed
Sampsell, B. M. & Held, W. A. (1985). Variation in the major urinary protein multigene family in wild-derived mice. Genetics 109, 549–568.CrossRefGoogle ScholarPubMed
Sanger, F., Coulson, A. R., Barrell, B. G., Swith, A. J. & Roe, B. A. (1980). Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. Journal of Molecular Biology 143, 161–178.CrossRefGoogle ScholarPubMed
Sanger, F., Nicklen, S. & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74, 5463–5467.CrossRefGoogle ScholarPubMed
Toole, J., Hastie, N. & Held, W. (1979). An abundant androgen-regulated mRNA in the mouse kidney. Cell 17, 441–448.CrossRefGoogle ScholarPubMed
Tronik, D., Dreyfus, M., Babinet, C. & Rougeon, F. (1987). Regulated expression of the Ren gene in transgenic mice derived from parental strains carrying only the Renl gene. EMBO Journal 6, 983–987.CrossRefGoogle Scholar
Watson, C. S., Salomon, D. & Catterall, J. F. (1984). Structure and expression of androgen-regulated genes in mouse kidney. Annals of the New York Academy of Science 438, 101–114.CrossRefGoogle ScholarPubMed