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A transposing forked-duplication with position effect variegation in Drosophila

Published online by Cambridge University Press:  14 April 2009

P. T. Shukla  and
C. Auerbach
P. T. Shukla
Affiliation:
Institute of Animal Genetics, Edinburgh
C. Auerbach
Affiliation:
Institute of Animal Genetics, Edinburgh
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In the course of an X-ray experiment, the normal allele of forked was transposed to the second chromosome, where it acts as a suppressor of forked. In this position, which is near the centromere, the duplication (Dp-f+) is subject to a variegated position effect. This was studied in its dependence on the hetero-euchromatin balance; the results agree with and extend those found for other position effects. In addition, we found regional preferences for variegation in the individual flies. The most interesting aspect of Dp-f+ is its tendency to transpose either to the homologous second chromosome or to Chromosome IV. In the latter position, Dp-f+ acts as a dominant near-lethal, so that the apparent selectivity of insertion sites is at least in part due to deleterious effects at insertion sites other than its original one. In a new, and presumably, centromere-far position of Dp-f+ on Chromosome II the variegated position effect disappeared and transposition was reduced in frequency or wholly abolished. The frequency of losses of Dp-f+ approximately equalled that of transpositions. Since there is good evidence that transpositions occurred pre-meiotically, the apparent losses of Dp-f+ may have been due to meiotic segregation separating the loss from the new insertion.

Type
Research Article
Information
Genetics Research , Volume 36 , Issue 1 , August 1980 , pp. 41 - 56
Copyright
Copyright © Cambridge University Press 1980

References

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