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Transfer-positive J-independent revertants of the F factor in Escherichia coli K 12

Published online by Cambridge University Press:  14 April 2009

Mark Achtman
Mark Achtman
Affiliation:
M.R.C. Molecular Genetics Research Unit, Department of Molecular Biology, University of Edinburgh, Edinburgh, Scotland
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Summary

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Cells carrying traJ− mutants of F are transfer-deficient and are good recipients in conjugation (Achtman, Willets & Clark, 1972). In addition, the traJ gene product is involved in pilus- and in plasmid-specificity (Willetts, 1971). J-independent mutants were isolated as revertants of a traJ− mutant; they still carry the traJ− mutation but also carry at least one other mutation which results in transfer in the absence of the traJ gene product. J-independent transfer of these mutants is not inhibited by the R 100 repressor. Various models are presented which can account for the properties of traJ− mutants and of these J-independent revertants.

Type
Research Article
Information
Genetics Research , Volume 21 , Issue 1 , February 1973 , pp. 67 - 77
Copyright
Copyright © Cambridge University Press 1973

References

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