Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-08T02:34:17.781Z Has data issue: false hasContentIssue false
  • English
  • Français

Transcription of rDNA insertions in bobbed mutants of Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

Régine Terracol
Régine Terracol
Affiliation:
Centre de Génétique Moléculaire*, C.N.R.S. 91190, Gif-sur-Yvette, France
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In Drosophila melanogaster a large number of the genes coding for 18S and 28S rRNA are interrupted in the 28S region by insertions of two types. Ribosomal insertion transcripts were compared in wild-type and bobbed strains. We found that the level of insertion transcripts increased in bobbed mutants after deletion of 50% of INS− genes, and inversely decreased in revertants when more than 50% of wild-type levels. Among type II insertion transcripts we found a predominant 3·5 kb RNA, precisely of the most frequent insertion size. No primary insertion transcript has been found, although it could be undetected if very fast splicing leads to mature 28S occurs.

Type
Research Article
Information
Genetics Research , Volume 48 , Issue 3 , December 1986 , pp. 167 - 174
Copyright
Copyright © Cambridge University Press 1986

References

Appels, R. & Hilliker, A. J. (1982). The cytogenetics boundaries of the rDNA region within heterochromatin of the X chromosome of Drosophila melanogaster and their relation to male meiotic pairing site. Genetical Research 39, 149–156.CrossRefGoogle Scholar
Birnboim, H. C. & Doly, J. (1979). A rapid alcaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7, 1513–1523.CrossRefGoogle Scholar
Dawid, I. B. & Botchan, P. (1977). Sequences homologous to ribosomal insertions occur in the Drosophila genome outside the nucleolus organizer. Proceedings of the National Academy of Sciences (U.S.A.) 74, 4233–4237.CrossRefGoogle ScholarPubMed
Dawid, I. B., Long, E. O., Dinocera, P. P. & Pardue, M. L. (1981). Ribosomal insertion-like elements in Drosophila melanogaster are interspersed with mobile sequences. Cell 25, 399–408.CrossRefGoogle ScholarPubMed
Dawid, I. B. & Rebbert, M. L. (1986). Expression of ribosomal insertion in Drosophila: sensitivity to intercalating drugs. Nucleic Acids Research 14, 1267–1277.CrossRefGoogle ScholarPubMed
Dawid, I. B., Wellauer, P. K. & Long, E. O. (1978). Ribosomal DNA in Drosophila melanogaster. I. Isolation and characterization of cloned fragments. Journal of Molecular Biology 126, 749–768.CrossRefGoogle ScholarPubMed
De Cicco, D. V. & Glover, D. M. (1983). Amplification of rDNA and type I sequences in Drosophila males deficient in rDNA. Cell 32, 1217–1225.CrossRefGoogle ScholarPubMed
Dunn, A. R. & Hassel, J. A. (1977). A novel method to map transcripts: evidence for homology between an Adenovirus mRNA and discrete multiple regions of the viral genome. Cell 12, 23–36.CrossRefGoogle ScholarPubMed
Glover, D. M. (1981). The rDNA of Drosophila melanogaster. Cell 26, 297–298.CrossRefGoogle ScholarPubMed
Glover, D. M. & Hogness, D. S. (1977). A novel arrangement of the 18S and 28S sequences in a repeating unit of Drosophila melanogaster. Cell 10, 167–176.CrossRefGoogle Scholar
Glover, D. M., White, R. L., Finnegan, D. J. & Hogness, D. S. (1975). Characterization of six cloned DNAs from Drosophila melanogaster, including one that contains the genes for rRNA. Cell 5, 149–157.CrossRefGoogle ScholarPubMed
Graziani, F. & Gargano, S. (1976). Ribosomal DNA transcription products during the first steps of magnification in Drosophila melanogaster. Journal of Molecular Biology 100, 59–71.CrossRefGoogle ScholarPubMed
Jamrich, M. & Miller, O. L. (1984). The rare transcripts of interrupted rRNA genes in Drosophila melanogaster are processed or degraded during synthesis. EMBO Journal 3, 1541–1545.CrossRefGoogle ScholarPubMed
Jolly, D. J. & Thomas, C. A. (1980). Nuclear RNA transcripts from Drosophila melanogaster ribosomal RNA genes containing introns. Nucleic Acids Research 8, 67–84.CrossRefGoogle ScholarPubMed
Kidd, S. J. & Glover, D. M. (1980). A DNA segment from D. melanogaster which contains five tandemly units homologous to the major rDNA insertion. Cell 19, 103–119.CrossRefGoogle Scholar
Kidd, S. J. & Glover, D. M. (1981). Drosophila melanogaster ribosomal DNA containing type II insertions is variably transcribed in different strains and tissues. Journal of Molecular Biology 151, 645–662.CrossRefGoogle ScholarPubMed
Labella, T., Vicari, L., Manzi, A. & Graziani, F. (1983). Expression of rDNA insertions during rDNA magnification in D. melanogaster. Molecular and General Genetics 190, 487–493.CrossRefGoogle ScholarPubMed
Locker, D. & Marrakechi, M. (1977). Evidence for an excess of rDNA in the testis of Drosophila melanogaster during rDNA magnification. Molecular and General Genetics 154, 249–254.CrossRefGoogle ScholarPubMed
Long, E. O., Collins, M., Kiefer, B. I. & Dawid, I. B. (1981). Expression of the ribosomal DNA insertions in bobbed mutants of Drosophila melanogaster Molecular and General Genetics 182, 377–384.CrossRefGoogle ScholarPubMed
Long, E. O. & Dawid, , (1979). Expression of ribosomal DNA insertions in Drosophila melanogaster. Cell 18, 1185–1196.CrossRefGoogle ScholarPubMed
Long, E. O., Rebbert, M. L. & Dawid, I. B. (1980). Structure and expression of ribosomal RNA genes of Drosophila melanogaster interrupted by type 2 insertions. Cold Spring Harbor Symposium of Quantitative Biology 45, 667–672.CrossRefGoogle Scholar
Marrakechi, M. (1974). Etude d'un mutant bobbed ayant une quantité normale d'ADN ribosomal. Molecular and General Genetics 135, 213–229.CrossRefGoogle Scholar
Marrakechi, M. & Prud'homme, N. (1971). A study of a mutant bobbed induced by ethyl methane sulfonate in Drosophila melanogaster. Biochemical and Biophysical Research Communications 43, 273–277.CrossRefGoogle ScholarPubMed
Peacock, W. J., Appels, R., Endow, S. & Glover, D. (1981). Chromosomal distribution of the major insert in Drosophila melanogaster 28S rRNA genes. Genetical Research 37, 209–214.CrossRefGoogle ScholarPubMed
Pelligrini, M., Manning, J. & Davidson, N. (1977). Sequence arrangement of the rDNA of Drosophila melanogaster. Cell 10, 213–224.CrossRefGoogle Scholar
Ritossa, F. M., Atwood, K. C. & Spiegelman, S. (1966). A molecular explanation of bobbed mutants of Drosophila as partial deficiencies of ribosomal DNA. Genetics 54, 819–834.CrossRefGoogle ScholarPubMed
Ritossa, F., Malva, C., Boncinelli, E., Graziani, F. & Polito, L. (1971). The first steps of magnification of DNA complementary to ribosomal RNA in Drosophila melanogaster. Proceedings of the National Academy of Sciences (U.S.A.) 68, 1580–1584.CrossRefGoogle Scholar
Shermoen, A. W. & Kiefer, B. I. (1975). Regulation in rDNA-deficient Drosophila melanogaster. Cell 4, 275–280.CrossRefGoogle ScholarPubMed
Terracol, R. & Prud'homme, N. (1981). 26S and 18S rRNA synthesis is bobbed mutants of Drosophila melanogaster Biochimie 63, 451–455.CrossRefGoogle ScholarPubMed
Terracol, R. & Prud'homme, N. (1986). Differential elimination of rDNA genes in bobbed mutants of Drosophila melanogaster. Molecular and Cellular Biology 6, 1023–1031.Google ScholarPubMed
Thomas, P. S. (1980). Hybridization of denatured DNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences (U.S.A.) 77, 5201–5205.CrossRefGoogle ScholarPubMed
Tullis, R. H. & Rubin, H. (1980). Calcium protects DNase I from proteinase K: a new method for the removal of contaminating RNase from DNase: I. Analytical Biochemistry 107, 260–264.CrossRefGoogle ScholarPubMed
Wahl, G. M., Stern, M. & Stark, G. R. (1979). Efficient transfer of large fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization using dextran sulfate. Proceedings of the National Academy of Sciences (U.S.A.) 76, 3683–3687.CrossRefGoogle ScholarPubMed
Wayne, R. L., Sharp, Z. D. & Procunier, J. D. (1985). Preferential DNase I sensitivity of insert-free ribosomal RNA of Drosophila melanogaster. Nucleic Acids Research 13, 2869–2879.CrossRefGoogle ScholarPubMed
Wellauer, P. K. & Dawid, I. B. (1977). The structural organization of ribosomal DNA in Drosophila melanogaster. Cell 10, 193–212.CrossRefGoogle ScholarPubMed
Wellauer, P. K. & Dawid, I. B. (1978). Ribosomal DNA in Drosophila melanogaster. II. Heteroduplex mapping of cloned and uncloned rDNA. Journal of Molecular Biology 126, 769–782.CrossRefGoogle ScholarPubMed
Wellauer, P. K., Dawid, I. B. & Tartof, K. D. (1978). X and Y chromosomal ribosomal DNA of Drosophila: comparison of spacers and insertions. Cell 14, 269–278.CrossRefGoogle ScholarPubMed
White, R. L. & Hogness, D. S. (1977). R loop mapping of 18S and 28S sequences in the long and short repeating units of Drosophila melanogaster rDNA. Cell 10, 177–192.CrossRefGoogle Scholar