Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-28T04:19:13.108Z Has data issue: false hasContentIssue false
  • English
  • Français

Three genes determine the carboxin sensitivity of mitochondrial succinate oxidation in Aspergillus nidulans

Published online by Cambridge University Press:  14 April 2009

I. A. U. N. Gunatilleke ,
H. N. Arst Jr  and
C. Scazzocchio
I. A. U. N. Gunatilleke
Affiliation:
Department of Genetics, University of Cambridge, Milton Road, Cambridge CB4 1XH, England
H. N. Arst Jr*
Affiliation:
Department of Genetics, University of Cambridge, Milton Road, Cambridge CB4 1XH, England
C. Scazzocchio
Affiliation:
Department of Genetics, University of Cambridge, Milton Road, Cambridge CB4 1XH, England
*
† Address correspondence to this author.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Partially dominant mutations to carboxin resistance occur in three, freely recombining, nuclear genes in Aspergillus nidulans. Mutations at all three loci reduce carboxin inhibition of succinate dehydrogenase (EC 1.3.99.1), succinate-cytochrome c reductase (EC 1.3.99.1) and succinate oxidase (EC 1.3.99.1) in mitochondrial preparations. It is therefore probable that the ability of carboxin to prevent growth of A. nidulans is a direct consequence of its ability to prevent succinate oxidation.

Type
Research Article
Information
Genetics Research , Volume 26 , Issue 3 , December 1975 , pp. 297 - 305
Copyright
Copyright © Cambridge University Press 1975

References

REFERENCES

Alderson, T. & Hartley, M. J. (1969). Specificity for spontaneous and induced mutation at several gene loci in Aspergillus nidulans. Mutation Research 8, 255–264.CrossRefGoogle ScholarPubMed
Arst, H. N. Jr & Cove, D. J. (1969). Methylammonium resistance in Aspergillus nidulans. Journal of Bacteriology 98, 1284–1293.CrossRefGoogle ScholarPubMed
Arst, H. N. Jr & Cove, D. J. (1973). Nitrogen metabolite repression in Aspergillus nidulans. Molecular and General Genetics 126, 111–141.CrossRefGoogle ScholarPubMed
Bailey, C. & Arst, H. N. Jr (1975). Carbon catabolite repression in Aspergillus nidulans. European Journal of Biochemistry 51, 573–577.CrossRefGoogle Scholar
Ben-Yephet, Y., Henis, Y. & Dinoor, A. (1975). Inheritance of tolerance to carboxin and benomyl in Ustilago hordei. Phytopathology 65, 563–567.CrossRefGoogle Scholar
Clutterbuck, A. J. (1974). Aspergillus nidulans. In Handbook of Genetics (ed. King, R. C.), 1, 447–510. New York: Plenum Press.Google Scholar
Cove, D. J. (1966). The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochimica et Biophysica Acta 113, 51–56.CrossRefGoogle ScholarPubMed
Flavell, R. B. & Woodward, D. O. (1970). The concurrent regulation of metabolically related enzymes. The Krebs cycle and glyoxylate shunt enzymes in Neurospora. European Journal of Biochemistry 17, 284–291.CrossRefGoogle ScholarPubMed
Georgopoulos, S. G., Alexandri, E. & Chrysayi, M. (1972). Genetic evidence for the action of oxathiin and thiazole derivatives on the succinic dehydrogenase system of Ustilago maydis mitochondria. Journal of Bacteriology 110, 809–817.CrossRefGoogle ScholarPubMed
Georgopoulos, S. G., Chrysayi, M. & White, G. A. (1975). Carboxin resistance in the haploid, the heterozygous diploid, and the plant-parasitic dicaryotic phase of Ustilago maydis. Pesticide Biochemistry and Physiology 5, 543–551.CrossRefGoogle Scholar
Georgopoulos, S. G. & Sisler, H. D. (1970). Gene mutation eliminating antimycin A-tolerant electron transport in Ustilago maydis. Journal of Bacteriology 103, 745–750.CrossRefGoogle ScholarPubMed
Gunatilleke, I. A. U. N., Scazzocchio, C. & Arst, H. N. Jr (1975). Cytoplasmic and nuclear mutations to chloramphenicol resistance in Aspergillus nidulans. Molecular and General Genetics 137, 269–276.CrossRefGoogle ScholarPubMed
King, T. E. (1967). Preparations of succinate-cytochrome c reductase and the cytochrome b-C 1 particle and reconstitution of succinate-cytochrome c reductase. Methods in Enzymology 10, 216–225.CrossRefGoogle Scholar
Lambowitz, A. M., Smith, E. W. & Slayman, C. W. (1972). Electron transport in Neurospora mitochondria. Studies on wild type and poky. Journal of Biological Chemistry 247, 4850–4858.CrossRefGoogle ScholarPubMed
Layne, E. (1957). Spectrophotometric and turbidimetric methods for measuring proteins. Methods in Enzymology 3, 447–454.CrossRefGoogle Scholar
McCully, K. S. & Forbes, B. (1965). The use of p-fluorophenylalanine with ‘master strains’ of Aspergillus nidulans for assigning genes to linkage groups. Genetical Research 6, 352–259.CrossRefGoogle ScholarPubMed
Pontecorvo, G., Roper, J. A., Hemmons, L. M., Macdonald, K. D. & Bufton, A. W. J. (1953). The genetics of Aspergillus nidulans. Advances in Genetics 5, 141–238.CrossRefGoogle ScholarPubMed
Ragsdale, N. N. & Sisler, H. D. (1970). Metabolic effects related to fungitoxicity of carboxin. Phytopathology 60, 1422–1427.CrossRefGoogle ScholarPubMed
Rowlands, R. T. & Turner, G. (1974). Physiological and biochemical studies of nuclear and extranuclear oligomycin-resistant mutants of Aspergillus nidulans. Molecular and General Genetics 132, 73–88.CrossRefGoogle ScholarPubMed
Ulrich, J. T. & Mathre, D. E. (1972). Mode of action of oxathiin systemic fungicides. V. Effect on electron transport system of Ustilago maydis and Saccharomyces cerevisiae. Journal of Bacteriology 110, 628–632.CrossRefGoogle ScholarPubMed
Veeger, C., DerVartanian, D. V. & Zeylemaker, W. P. (1969). Succinate dehydrogenase. Methods in Enzymology 13, 81–90.CrossRefGoogle Scholar
Weiss, H., von Jagow, G., Klingenberg, M. & Bücher, T. (1970). Characterization of Neurospora crassa mitochondria prepared with a grind-mill. European Journal of Biochemistry 14, 75–82.CrossRefGoogle ScholarPubMed
Wharton, D. C. & Tzagoloff, A. (1967). Cytochrome oxidase from beef heart mitochondria. Methods in Enzymology 10, 245–250.CrossRefGoogle Scholar
White, G. A. (1971). A potent effect of 1,4-oxathiin systemic fungicides on succinate oxidation by a particulate preparation from Ustilago maydis. Biochemical and Biophysical Research Communications 44, 1212–1219. (See also erratum in Biochemical and Biophysical Research Communications 56, 282 (1974).)CrossRefGoogle ScholarPubMed
White, G. A. & Thorn, G. D. (1975). Structure-activity relationships of carboxamide fungicides and the succinic dehydrogenase complex of Cryptococcus laurentii and Ustilago maydis. Pesticide Biochemistry and Physiology 5, 380–395.CrossRefGoogle Scholar