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Studies of aromatic biosynthetic and catabolic enzymes in Ustilago maydis and in mutants of U. violacea

Published online by Cambridge University Press:  14 April 2009

Mary B. Berlyn  and
Norman H. Giles
Mary B. Berlyn
Affiliation:
Department of Biology, Yale University
Norman H. Giles
Affiliation:
Department of Biology, Yale University
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A multienzyme complex for five of the enzymes in the prechorismate portion of the aromatic biosynthetic pathway has been demonstrated in Ustilago violacea, and has previously been reported in U. maydis (Ahmed & Giles, 1969). This complex is similar to that found in Neurospora crassa and other fungi. In U. violacea polyaromatic-requiring mutants show pleiotropic deficiencies for all five of these enzymes, similar to the extreme pleiotropic polar mutants of the arom gene cluster in Neurospora (Giles, Case, Partridge & Ahmed, 1967a; Case & Giles, 1971). This result is interpreted as mutational evidence for an arom gene cluster in U. violacea comparable to that in N. crassa. A second low molecular weight, heat-stable isozyme of dehydroquinase is shown to be present at high (constitutive) levels in U. maydis, as previously indicated by Ahmed & Giles (1969), but this activity is increased to extraordinarily high levels in cells grown in the presence of quinate. In contrast, U. violacea strains do not grow on quinate, have a single, heat-labile dehydroquinase species, and lack activities for other enzymes in the quinate catabolic pathway.

Type
Research Article
Information
Genetics Research , Volume 19 , Issue 3 , June 1972 , pp. 261 - 270
Copyright
Copyright © Cambridge University Press 1972

References

REFERENCES

Ahmed, S. I. & Giles, N. H. (1969). Organization of enzymes in the common aromatic synthetic pathway: evidence for aggregation in fungi. Journal of Bacteriology 99, 231–237.Google Scholar
Berlyn, M. B., Ahmed, S. I. & Giles, N. H. (1970). Organization of polyaromatic biosynthetic enzymes in a variety of photosynthetic organisms. Journal of Bacteriology 104, 768–774.Google Scholar
Berlyn, Mary B. & Giles, N. H. (1969). Organization of enzymes in the polyaromatic biosynthetic pathway: separability in bacteria. Journal of Bacteriology 99, 222–230.Google Scholar
Carles, J. (1957). L'evolution des acides organiques du maïs. Bulletin de la Societé de Chimie Biologique 39, 1177–1182.Google Scholar
Case, Mary E. & Giles, N. H. (1968). Evidence for nonsense mutations in the arom gene cluster of Neurospora crassa. Genetics 60, 49–58.Google Scholar
Case, Mary E. & Giles, N. H. (1971). Partial enzyme aggregates formed by pleiotropic mutants in the arom gene cluster of Neurospora crassa. Proceedings of the National Academy of Sciences, U.S.A. 68, 58–62.Google Scholar
Chaleff, R. (1971). Evidence for a gene cluster controlling the inducible quinate catabolic pathway in Neurospora crassa. Genetics 68s, 10 (Abst.).Google Scholar
Day, A. W. & Jones, J. K. (1968). The production and characteristics of diploids in Ustilago violacea. Genetical Research 11, 63–81.Google Scholar
Day, A. W. & Jones, J. K. (1969). Sexual and parasexual analysis of Ustilago violacea. Genetical Research 14, 195–221.Google Scholar
DeLeeuw, A. (1968). Gene-enzyme relationships in aromatic biosynthesis in yeast. Ph.D. thesis, Yale University.Google Scholar
Giles, N. H., Case, Mary E., Patridge, C. W. H. & Ahmed, S. I. (1967 a). A gene cluster in Neurospora crassa coding for an aggregate of five aromatic synthetic enzymes. Proceedings of the National Academy Sciences, U.S.A. 58, 1453–1460.Google Scholar
Giles, N. H., Partridge, C. W. H., Ahmed, S. I. & Case, M. E. (1967 b). The occurrence of two dehydroquinases in Neurospora crassa, one constitutive and one inducible. Proceedings of the National Academy of Sciences, U.S.A. 58, 1930–1937.Google Scholar
Holliday, R. (1961). The genetics of Ustilago maydis. Genetical Research 2, 231–248.CrossRefGoogle Scholar
Holliday, R. (1962). Selection of auxotrophs by inositol starvation in Ustilago maydis. Microbial Genetics Bulletin No. 18, pp. 28–30.Google Scholar
Rines, H. W. (1969). Genetical and biochemical studies on the inducible quinic acid catabolic pathway in Neurospora crassa. Ph.D. thesis, Yale University.Google Scholar