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Structure, frequency and distribution of P elements in relation to P—M hybrid dysgenic male recombination in Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

K. A. Exley  and
P. Eggleston
K. A. Exley
Affiliation:
Department of Genetics and Microbiology, University of Liverpool, P.O. Box 147, Liverpool L69 3BX
P. Eggleston
Affiliation:
Department of Genetics and Microbiology, University of Liverpool, P.O. Box 147, Liverpool L69 3BX
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The frequency and distribution of P elements were investigated in the third chromosomes of two wild-type strains of Drosophila melanogaster using in situ hybridization of biotinylated probes to the polytene chromosomes. The relationship between these data and the extent of hybrid dysgenesis was determined through assays of egg production, egg hatchability (F2 embryo lethality), snw destabilization and male recombination along the third chromosome. The results suggest that P-element distribution, frequency and structure are all contributory factors in the regulation of hybrid dysgenesis. Texas 6 was shown consistently to be a stronger P strain than Texas 1, eliciting greater reductions in fertility, more extensive snw destabilization and higher frequencies of male recombination. Clustering of male recombination events, arising from pre-meiotic crossing over, was evident among the dysgenic progeny of each strain. Male recombination and snw destabilization were independently distributed among the dysgenic males studied, suggesting that these traits represent separate P-mediated functions. The third chromosome male recombination maps produced by the two strains differed significantly from each other and from the published female meiotic and polytene chromosome maps. Male recombination breakpoints were associated with the original distribution of P sequences in the two strains and the results suggest that this relationship may be closer for potentially complete P factors than for P sequences in general. An analysis of sub-lines derived from individual recombinant males revealed that chromosomal breakpoints could also be associated with novel insertions following P-element transposition.

Type
Research Article
Information
Genetics Research , Volume 53 , Issue 3 , June 1989 , pp. 163 - 171
Copyright
Copyright © Cambridge University Press 1989

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