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Selection of lon mutants in Escherichia coli by treatment with phenothiazines

Published online by Cambridge University Press:  14 April 2009

J. Molnár ,
I. B. Holland  and
Yvette Mándi
J. Molnár
Affiliation:
Institute of Microbiology, University Medical School, Szeged, Hungary
I. B. Holland
Affiliation:
Institute of Microbiology, University Medical School, Szeged, Hungary
Yvette Mándi
Affiliation:
Institute of Microbiology, University Medical School, Szeged, Hungary
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Growth of E. coli K12 in the presence of chlorpromazine or promethazine resulted in the appearance of a special kind of mutational change with a frequency of 5–10%. These cells were UV-sensitive and very mucoid in their colony formation. The strains were characterized as colanic acid producing on the basis of phage m-59 sensitivity and we tentatively conclude that these mutants are Lon−.

In another series of experiments drug treatment of E. coli failed to yield significant numbers of auxotrophs and the back mutation rate of a number of S. typhimurium His− strains was not significantly increased.

Type
Research Article
Information
Genetics Research , Volume 30 , Issue 1 , August 1977 , pp. 13 - 20
Copyright
Copyright © Cambridge University Press 1977

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