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A search for temperature-sensitive mutants of Ustilago maydis blocked in DNA synthesis

Published online by Cambridge University Press:  14 April 2009

P. Unrau  and
R. Holliday
P. Unrau
Affiliation:
National Institute for Medical Research, Mill Hill, London, N.W.7
R. Holliday
Affiliation:
National Institute for Medical Research, Mill Hill, London, N.W.7
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Over 400 temperature-sensitive mutants of Ustilago maydis have been tested for DNA synthesis at the restrictive temperature of 32°C by measuring 14C adenine incorporation into DNA and RNA. Five mutants were defective in DNA synthesis but none was completely blocked. One mutant, tsd-1, which is unlinked to the others, forms long uninucleate filaments at 32°C which die exponentially after 4h temperature treatment. The phenotype is comparable to that of thymine-starved bacteria, but it is possible that rather than being specifically defective in DNA synthesis the mutant is blocked in nuclear division.

Type
Research Article
Information
Genetics Research , Volume 15 , Issue 2 , April 1970 , pp. 157 - 169
Copyright
Copyright © Cambridge University Press 1970

References

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