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Search for ribosomal mutants in Podospora anserina: genetic analysis of cold-sensitive mutants

Published online by Cambridge University Press:  14 April 2009

Marguerite Picard-Bennoun  and
Denise Le Coze
Marguerite Picard-Bennoun
Affiliation:
Laboratoire de Génétique de l' Université Paris-Sud, Centre d'ORSAY (Laboratoire associé au CNRS n° 86) et Laboratoire de Génétique de l'Université Bordeaux II Bâtiment 400, 91405 Orsay, France
Denise Le Coze
Affiliation:
Laboratoire de Génétique de l' Université Paris-Sud, Centre d'ORSAY (Laboratoire associé au CNRS n° 86) et Laboratoire de Génétique de l'Université Bordeaux II Bâtiment 400, 91405 Orsay, France
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Twenty-four cold-sensitive (prototrophic) mutants were isolated after UV mutagenesis of protoplasts of the fungus Podospora anserina. Genetic analysis of these mutants was performed in order to detect those among them which were most likely to be impaired in translational fidelity. The 24 mutations belonged to 24 different genes. One half of the mutants were pleiotropic and displayed an altered phenotype: growth rate at the permissive temperature, germination of the spores, fertility and/or sporulation. Nine mutants differed from wild-type in their resistance levels to cycloheximide, trichodermin and/or paromomycin. Several mutations were linked to known ribosomal loci. Two mutations behaved like informational antisuppressors: one is allelic to the previously described As3 gene and the other one defines a new antisuppressor gene, AS6.

Type
Research Article
Information
Genetics Research , Volume 36 , Issue 3 , December 1980 , pp. 289 - 297
Copyright
Copyright © Cambridge University Press 1980

References

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