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R.SmaI cleavage map of the transfer region of the E. coli K12 sex factor F

Published online by Cambridge University Press:  14 April 2009

Neil Willetts  and
Gillian Johnson
Neil Willetts
Affiliation:
Department of Molecular Biology, University of Edinburgh, Kings Buildings, Edinburgh EH9 3JR, Scotland
Gillian Johnson
Affiliation:
Department of Molecular Biology, University of Edinburgh, Kings Buildings, Edinburgh EH9 3JR, Scotland
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Summary

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A map of the seventeen R.SmaI sites within the 33·4 kb transfer region of plasmid F has been obtained. The number of R.SmaI sites contrasts sharply with the few sites for other restriction endonucleases with hexanucleotide specificity. Location of the R.SmaI sites in γtra transducing phages allowed physical location of lambda insertions into the traB, traF and traH genes.

Type
Short papers
Information
Genetics Research , Volume 34 , Issue 2 , October 1979 , pp. 195 - 202
Copyright
Copyright © Cambridge University Press 1979

References

REFERENCES

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