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Role of simple and complex aggregates in Escherichia coli Hfr × F− matings

Published online by Cambridge University Press:  14 April 2009

Paul Broda  and
J. F. Collins
Paul Broda
Affiliation:
Department of Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland
J. F. Collins
Affiliation:
Department of Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland
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Summary

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Analysis of tetraparental Hfr × F− matings of Escherichia coli strains showed that mating complexes were predominantly pairs or small aggregates of cells. Many physically associated complexes of donor and recipient cells gave rise to no recombinant cells. The observed linkage of genetic markers in recombinants is sensitive to multiple mating events, and should therefore be measured under well-defined conditions giving pairwise matings only.

Type
Research Article
Information
Genetics Research , Volume 31 , Issue 2 , April 1978 , pp. 167 - 175
Copyright
Copyright © Cambridge University Press 1978

References

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