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R-Factor elimination by thymine starvation

Published online by Cambridge University Press:  14 April 2009

R. J. Pinney  and
J. T. Smith
R. J. Pinney
Affiliation:
Microbiology Section, Department of Pharmaceutics, The School of Pharmacy, University of London, Brunswick Square, London, WC1N 1AX
J. T. Smith
Affiliation:
Microbiology Section, Department of Pharmaceutics, The School of Pharmacy, University of London, Brunswick Square, London, WC1N 1AX
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Summary

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R-factor 1818, which is refractory to curing by acridines or ethidium bromide from Escherichia coli K 12 and Klebsiella aerogenes 418 hosts, was eliminated from thymineless mutants of these organisms under conditions of thymine starvation. Neither R-7268 nor R-TEM could be eliminated by this method. The concurrence of R-TEM with R-1818 inhibited the curing of R-1818, whereas R-7268 had no effect on R-1818 elimination

Type
Research Article
Information
Genetics Research , Volume 18 , Issue 2 , October 1971 , pp. 173 - 177
Copyright
Copyright © Cambridge University Press 1971

References

REFERENCES

Alikhanian, S. I., Iijina, T. S., Kaliaeva, E. S., Kameneva, S. V. & Sukhodelec, V. V. (1966). A genetic study of thymineless mutants of E. coli K12. Genetical Research 8, 83–100.CrossRefGoogle ScholarPubMed
Barner, H. E. & Cohen, S. S. (1954). The induction of thymine synthesis by T2 infection of a thymine requiring mutant of Escherichia coli. Journal of Bacteriology 68, 80–88.CrossRefGoogle ScholarPubMed
Bouanchaud, D. H., Scavizzi, M. R. & Chabbert, Y. A. (1969). Elimination by ethidium bromide of antibiotic resistance in Enterobacteria and Staphylococci. Journal of General Microbiology 54, 417–425.CrossRefGoogle Scholar
Clowes, R. C., Moody, E. E. M. & Pritchard, R. H. (1965). The elimination of extra-chromosomal elements in thymineless strains of Escherichia coli K12. Genetical Research 6, 147–152.CrossRefGoogle Scholar
Cummings, D. J. & Kusy, A. R. (1969). Thymineless death in Escherichia coli: inactivation and recovery. Journal of Bacteriology 99, 558–566.CrossRefGoogle ScholarPubMed
Datta, N. & Kontomichalou, P. (1965). Penicillinase synthesis controlled by infectious R-factors in Enterobacteriaceae. Nature 208, 239–241.CrossRefGoogle ScholarPubMed
Davis, B. D. & Mingioli, E. S. (1950). Mutants of Escherichia coli requiring methionine or vitamin B 12. Journal of Bacteriology 60, 17–28.CrossRefGoogle ScholarPubMed
Freifelder, D. (1969). Single-strand breaks in bacterial DNA associated with thymine starvation. Journal of Molecular Biology 45, 1–7.CrossRefGoogle ScholarPubMed
Hirota, Y. & Iijima, T. (1957). Acriflavine as an effective agent for eliminating R-factor in Escherichia coli K12. Nature 180, 655–656.CrossRefGoogle Scholar
Meynell, E. & Datta, N. (1966). The relation of resistance transfer factors to the F-factor (sex-factor) of Escherichia coli K12. Genetical Research 7, 134–140.CrossRefGoogle Scholar
Smith, J. T. (1967). Production of thymineless mutants in Gram-negative bacteria (Aerobacter, Proteus). Journal of General Microriology 47, 131–137.CrossRefGoogle ScholarPubMed
Smith, J. T. (1969). R-factor gene expression in Gram-negative bacteria. Journal of General Microriology 55, 109–120.CrossRefGoogle ScholarPubMed
Smith, J. T. & Hamilton-Miller, J. M. T. (1963). Differences between penicillinases from Gram-positive and Gram-negative bacteria. Nature 197, 976–978.CrossRefGoogle ScholarPubMed
Tomoeda, M., Inzuka, M., Kubo, N. & Nakamura, S. (1968). Effective elimination of drug resistance and sex factors in Escherichia coli by sodium dodecyl sulphate. Journal of Bacteriology 95, 1078–1089.CrossRefGoogle Scholar
Walker, J. R. (1970). Thymine starvation and single-strand breaks in chromosomal deoxyribonucleic acid of Escherichia coli. Journal of Bacteriology 104, 1391–1392.CrossRefGoogle ScholarPubMed
Watanabe, T. & Fukasawa, T. (1961). Episome mediated transfer of drug resistance in Enterobacteriaceae. II. Elimination of resistance factors by acridine dyes. Journal of Bacteriology 81, 679–683.CrossRefGoogle ScholarPubMed
Weissbach, A. & Shuster, R. C. (1969). The biosynthesis of phage lambda DNA. Bulletin de la Société de chimie biologique 51, 1385–1395.Google ScholarPubMed