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Restriction enzyme banding in Atlantic salmon (Salmo salar) and brown trout (Salmo trutta)

Published online by Cambridge University Press:  14 April 2009

S. E. Hartley
S. E. Hartley
Affiliation:
Department of Biological and Molecular Sciences, School of Natural Sciences, University of Stirling, Stirling FK9 4 LA, Scotland
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Summary

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Fixed metaphase chromosomes of brown trout and Atlantic salmon were digested with various restriction enzymes and stained with Giemsa. C band-like patterns were produced in both species by Alu I, Dde I, Hae III and Mbo I. Alu I revealed extra chromosome bands in brown trout which allowed identification of additional chromosome pairs, while the other three enzymes produced patterns identical to C banding. In the Atlantic salmon Dde I revealed telomeric bands at all telomeres in addition to the conventional C bands and all four enzymes had differential effects on the nucleolar organizer-associated heterochromatin. The relevance of these findings to chromosome identification and constitutive heterochromatin organization in salmonid fishes is discussed.

Type
Research Article
Information
Genetics Research , Volume 57 , Issue 3 , June 1991 , pp. 273 - 278
Copyright
Copyright © Cambridge University Press 1991

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