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Resistance to p-fluorophenylalanine in diploid/haploid dikaryons: dominance modifier gene explained as a controller of hybrid multimer formation

Published online by Cambridge University Press:  14 April 2009

D. Lewis  and
Dina Vakeria
D. Lewis
Affiliation:
Department of Botany & Microbiology, University College London, Gower Street, London WCJE 6BT
Dina Vakeria
Affiliation:
Department of Botany & Microbiology, University College London, Gower Street, London WCJE 6BT
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Summary

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The gene pfpr-10 in Coprinus confers resistance to p-fluorophenylalanine. The resistance of the heterozygote pfpr-10/pfp8-10 is completely recessive, both in diploids and dikaryons. A dominance modifier Mod+ makes pfpr-10 dominant in a dikaryon but not in a cliploid. It also enhances the degree of resistance in r homozygotes. These results are explained on a hypothetical model based upon a hexameric product of the pfpr-10 gene, with resistance to PFP being proportional to the percentage of homo r hexamers. The Mod+ is presumed to act by keeping the r and s products separate so that hybrid multimers are reduced to a minimum. Critical threshold concentrations of PFP for diploid/haploid dikaryons in 40 different combinations of pfpr and Mod+ genes cover a wide range of gene doses and percentage of homo r multimers. The relationship between the critical threshold concentrations and the calculated percentage of homo r multimers supports the model.

Type
Research Article
Information
Genetics Research , Volume 30 , Issue 1 , August 1977 , pp. 31 - 43
Copyright
Copyright © Cambridge University Press 1977

References

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