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Resistance of Escherichia coli to penicillins: II. An improved mapping of the ampA gene

Published online by Cambridge University Press:  14 April 2009

Kerstin G. Eriksson-Grennberg
Kerstin G. Eriksson-Grennberg
Affiliation:
Department of Microbiology, University of Umeå, Umeå 6, Sweden
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The first mutation step towards ampicillin resistance in Escherichia coli occurs in the ampA gene, and gives resistance to a D, L-ampicillin concentration of 10 μg/ml. Using interrupted conjugation and transduction experiments ampA was found to be located at 82 min on the time scale of Taylor & Thoman (1964). A number of adjacent markers were studied and the probable gene order of the ampA region was found to be argH-metA-uvrA-ampA-purA-fdp-pyrB. Two independent alleles of ampA were cotransduced with fdp+ and purA+. The phenotypic expression of ampA in a purA strain has been investigated.

Type
Research Article
Information
Genetics Research , Volume 12 , Issue 2 , October 1968 , pp. 147 - 156
Copyright
Copyright © Cambridge University Press 1968

References

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