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Repressor-minus and operator-constitutive de-repressed mutants of F-like R factors: their effect on chromosomal transfer by HfrC

Published online by Cambridge University Press:  14 April 2009

Elinor Meynell  and
Marylyn Cooke
Elinor Meynell
Affiliation:
Department of Microbiology and Guinness–Lister Research Unit, Lister Institute of Preventive Medicine, Chelsea Bridge Road, London, S. W. 1
Marylyn Cooke
Affiliation:
Department of Microbiology and Guinness–Lister Research Unit, Lister Institute of Preventive Medicine, Chelsea Bridge Road, London, S. W. 1
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Certain drd mutants of fi+ R factors, when carried by strain HfrC, allowed polarized transfer of the Hfr chromosome to occur at the normal rate. These mutants were independently shown to be repressor-sensitive and so owed their de-repression to failure to produce represser (i−). With other drd mutants, independently shown to be repressor-insensitive (0c), the rate of polarized chromosome transfer was as low as with the wild type and only R pili were produced by the HfrC+ bacteria. These R factors, therefore, continued to produce repressor and the donor behaviour of an Hfr strain depends on functioning of the integrated F.

Type
Research Article
Information
Genetics Research , Volume 14 , Issue 3 , December 1969 , pp. 309 - 313
Copyright
Copyright © Cambridge University Press 1969

References

REFERENCES

Beckwith, J. (1967). Regulation of the lac operon. Science, N.Y. 156, 597–604.CrossRefGoogle ScholarPubMed
Cooke, M. & Meynell, E. (1969). Chromosomal transfer mediated by de-repressed R factors in F−Escherichia coli K 12. Genet. Res., Camb. 14, 79–87.CrossRefGoogle Scholar
Datta, N., Lawn, A. M. & Meynell, E. (1966). The relationship of F type piliation and F phage sensitivity to drug resistance transfer in R+F−Escherichia coli K 12. J. gen. Microbiol. 45, 365–376.CrossRefGoogle Scholar
Egawa, R. & Hirota, Y. (1962). Inhibition of fertility by multiple drug resistance factor in Escherichia coli K 12. Jap. J. Genet. 37, 66–69.CrossRefGoogle Scholar
Frydman, A. & Meynell, E. (1969). Interactions between de-repressed F-like R factors and wild type colicin B factors: superinfection immunity and represser susceptibility. Genet. Res., Camb. 14, 315–322.CrossRefGoogle Scholar
Lawn, A. M. (1967). Simple immunologieal labelling method for electron microscopy and its application to the study of filamentous appendages of bacteria. Nature, Lond. 214, 1151–1152.CrossRefGoogle Scholar
Meynell, E. & Datta, N. (1967). Mutant drug resistance factors of high transmissibility. Nature, Lond. 214, 885–887.CrossRefGoogle ScholarPubMed
Meynell, E., Meynell, G. G. & Datta, N. (1968). Phylogenetic relationships of drug resistance factors and other transmissible bacterial plasmids. Bact. Rev. 32, 55–83.CrossRefGoogle ScholarPubMed
Nishimura, Y., Ishibashi, M., Meynell, E. & Hirota, Y. (1967). Specific piliation directed by a fertility factor and a resistance factor of Escherichia coli. J. gen. Microbiol. 49, 89–98.CrossRefGoogle Scholar
Pearce, L. E. & Meynell, E. (1968). Specific chromosomal affinity of a Resistance Factor. J. gen. Microbiol. 50, 159–172.CrossRefGoogle Scholar
Romero, E. & Meynell, E. (1969). Covert fi-R factors in fi+R+ strains of bacteria. J. Bact. 97, 780–786.CrossRefGoogle ScholarPubMed
Sugino, Y. & Hirota, Y. (1962). Conjugal fertility associated with resistance factor R in Escherichia coli. J. Bact. 84, 902–910.CrossRefGoogle ScholarPubMed
Taylor, A. L. & Trotter, C. D. (1967). Revised linkage map of Escherichia coli. Bact. Rev. 31, 332–353.CrossRefGoogle ScholarPubMed
Watanabe, T. (1963). Infective heredity of multiple drug resistance in bacteria. Bact. Rev. 27, 87–115.CrossRefGoogle ScholarPubMed
Watanabe, T. & Fukasawa, T. (1962). Episome-mediated transfer of drug resistance in Enterobacteriaceae. IV. Interactions between Resistance Transfer Factor and F-factor in Escherichia coli K12. J. Bact. 83, 727–735.CrossRefGoogle Scholar