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Re-establishment of breeding stocks of mutant and inbred strains of mice from embryos stored at –196 °C for prolonged periods

Published online by Cambridge University Press:  14 April 2009

D. G. Whittingham ,
Mary F. Lyon  and
P. H. Glenister
D. G. Whittingham
Affiliation:
MRC Mammalian Development Unit, Wolfson House, (University College London), 4 Stephenson Way, London, NW1 2HE
Mary F. Lyon
Affiliation:
MRC Radiobiology Unit, Harwell, Didcot, Oxon, OX11 0RD
P. H. Glenister
Affiliation:
MRC Radiobiology Unit, Harwell, Didcot, Oxon, OX11 0RD
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Breeding stocks were re-established from embryos of various mutant and inbred strains of mice after prolonged storage in liquid nitrogen at the 8-cell stage even with strains where only a proportion of the progeny were of the desired type, i.e. in the ModP and XO strains. Gonadotrophin treatment failed to produce superovulation consistently in any of the strains tested. Although the initial survival of embryos after thawing and culture to the morula and blastocyst stage was highest for embryos from XO mothers (61%), these embryos suffered the heaviest early postimplantation loss after transfer (61%). The proportion of embryos, originally frozen, developing into foetuses and offspring was variable (13% HT, 14% PT, 20% ModP, 14% XO, 21% CBA and CBA-T6) and lower than previously reported for hybrid 3H1 embryos (20–30%). The sex ratio of the liveborn young was within the normal expected limits except for the Modp strain, where it differed significantly from the exected 2♂:1♀ ratio. The proportion of young of the desired type from the frozen embryos of Modp and XO females was less than expected (17 and 3% respectively). In all cases a normal breeding stock was reestablished whose performance was within normal limits for each strain. Even without further improvements in embryo collection and the freezing technique per se, the storage of embryos in liquid nitrogen is an extremely economic way of preserving mouse genetic stocks.

Type
Research Article
Information
Genetics Research , Volume 30 , Issue 3 , December 1977 , pp. 287 - 299
Copyright
Copyright © Cambridge University Press 1977

References

REFERENCES

Biggers, J. D., Whitten, W. K. & Whittingham, D. G. (1971). The culture of mouse embryos in vitro. In Methods in Mammalian Embryology (ed. Daniel, J. C. Jr), pp. 86–116. San Francisco: Freeman.Google Scholar
Burgoyne, P. S. & Biggers, J. D. (1976). The consequences of X-dosage deficiency, in the germ line: impaired development in vitro of preimplantation embryos from XO mice. Developmental Biology 51, 109–117.Google Scholar
Gates, A. H. (1965). Rate of ovular development as a factor in embryonic survival. In Preimplantation Stages of Pregnancy (ed. Wolstenholme, G. E. W. and Maeve, O'Connor), pp. 270–293. Ciba Foundation Symposium. London: Churchill.Google Scholar
Gates, A. H. (1971). Maximizing yield and developmental uniformity of eggs. In Methods in Mammalian Embryology (ed. Daniel, J. C. Jr), pp. 64–75. San Francisco: Freeman.Google Scholar
Lyon, M. F. (1975). Implications of freezing for the preservation of genetic stocks. In Basic Aspects of Freeze Preservation of Mouse Strains (ed. Mühlbock, O.), pp. 57–64. Gustav Fischer Verlag, Stuttgart, Germany.Google Scholar
Lyon, M. F. & Hawkes, S. G. (1973). Reproductive lifespan in irradiated and unirradiated chromosomally XO mice. Oenetical Research 21, 185–194.Google ScholarPubMed
Lyon, M. F., Whittingham, D. G. & Glenister, P. H. (1977). Long-term storage of frozen mouse embryos under increased background irradiation. In The Freezing of Mammalian Embryos (ed. Elliott, K. and Whelan, J.), pp. 273–282. Ciba Foundation Symposium No. 52 (new series). Amsterdam: Elsevier/Excerpta Medica/North-Holland.Google Scholar
Morris, T. (1968). The XO and OY chromosome constitutions in the mouse. Genetical Research 12, 125–137.Google Scholar
Whitten, W. K. & Dagg, C. P. (1961). Influence of spermatozoa on the cleavage rate of mouse eggs. Journal of Experimental Zoology 148, 173–183.Google Scholar
Whittingham, D. G. (1971). Culture of mouse ova. Journal of Reproduction and Fertility (Supplement) 14, 7–21.Google Scholar
Whittingham, D. G. (1974). Embryo banks in the future of developmental genetics. Genetics (Supplement) 78, 395–402.CrossRefGoogle ScholarPubMed
Whittingham, D. G. (1975). Low temperature storage of mammalian embryos. In Basic Aspects of Freeze Preservation of Mouse Strains (ed. Mühlbock, O.), pp. 45–55. Gustav Fischer Verlag, Stuttgart, Germany.Google Scholar
Whittingham, D. G. (1976). General aspects of egg culture and preservation. In Egg Transfer in Cattle (ed. Rowson, L. E. A.), pp. 101–116. E.E.C. Commission, Luxembourg and H.M.S.O. London.Google Scholar
Whittingham, D. G. (1977). Some factors affecting embryo storage in laboratory animals. In The Freezing of Mammalian Embryos (ed. Elliott, K. and Whelan, J.), pp. 97–108. Ciba Foundation Symposium No. 52 (new series). Amsterdam: Elsevier/Excerpta Medica/North-Holland.CrossRefGoogle Scholar
Whittingham, D. G., Leibo, S. P. & Mazur, P. (1972). Survival of mouse embryos frozen to – 196 °C and – 269 °C. Science 178, 411–414.Google Scholar
Whittingham, D. G., Lyon, M. F. & Glenister, P. H. (1977). Long-term storage of mouse embryos at – 196 °C: the effect of background radiation. Genetical Research 29, 171–181.Google Scholar
Whittingham, D. G. & Whitten, W. K. (1974). Long-term storage and aerial transport of frozen mouse embryos. Journal of Reproduction and Fertility 36, 433–435.Google Scholar
Wilmut, I. (1972). The effect of cooling rate, warming rate, cryoprotective agent and stage of development on survival of mouse embryos during freezing and thawing. Life Sciences 11, 1071–1079.Google Scholar