Hostname: page-component-745bb68f8f-5r2nc Total loading time: 0 Render date: 2025-01-12T22:48:15.879Z Has data issue: false hasContentIssue false
  • English
  • Français

Recombination events that span sites within neighbouring gene loci of Neurospora*

Published online by Cambridge University Press:  14 April 2009

Noreen E. Murray
Noreen E. Murray
Affiliation:
Department of Biological Sciences, Stanford University, Stanford, California; Botany School, University of Cambridge, England, and Department of Molecular Biology, University of Edinburgh, Scotland
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Evidence is given that me-7 and me-9 are separate but contiguous gene loci.

The pattern of polarized recombination throughout the me-7 me-9 region indicates the location of a recombinational discontinuity between the known me-7 alleles and the known me-9 alleles.

Recombination events may include sites in both genes but such events are not preferentially associated with parental combinations of flanking markers.

Recombination events extending into both genes provide an extra criterion for the ordering of the sites within the me-7 locus. The order so obtained confirms that deduced from the flanking markers of me+ recombinants from me-7 × me-7 crosses.

In me-7 × me-9 crosses but not in me-7 × me-7 crosses, the map order derived on the assumption that single exchanges are more frequent than apparent triple exchanges is the reverse of that derived from prototroph frequencies. It is concluded that the former criterion is more likely to have provided the correct order and the anomalous prototroph frequencies reflect the polarity of gene conversion within me-7.

Type
Research Article
Information
Genetics Research , Volume 15 , Issue 1 , February 1970 , pp. 109 - 121
Copyright
Copyright © Cambridge University Press 1970

References

REFERENCES

Case, M. E. & Giles, N. H. (1964). Allelic recombination in Neurospora: Tetrad analysis of a three-point cross within the pan-2 locus. Genetics, Princeton 49, 529–540.Google Scholar
Case, M. E. & Giles, N. H. (1968). Evidence for nonsense mutations in the arom gene cluster of Neurospora crossa. Genetics, Princeton 60, 49–58.Google Scholar
Dubes, G. R. (1953). Investigations of some ‘unknown’ mutants of Neurospora crassa. Ph.D. Thesis, California Institute of Technology, Pasadena.Google Scholar
Fogel, S. & Mortimer, R. K. (1969). Informational transfer in meiotic gene conversion. Proc. Natn. Acad. Sci., U.S.A. 62, 96–103.Google Scholar
Hastings, P. J. & Whitehouse, H. L. K. (1964). A polaron model of genetic recombination by the formation of hybrid DNA. Nature, Lond. 201, 1052–1054.CrossRefGoogle Scholar
Holliday, R. (1964). A mechanism for gene conversion in fungi. Genet. Res., Camb. 5, 282–304.Google Scholar
Holliday, R. (1968). Genetic recombination in fungi. Replication and recombination of genetic material. Canberra, pp.157–174.Google Scholar
Kerr, D. & Flavin, M. (1969). The regulation of methionine synthesis and the nature of cystathionine γ-synthase in Neurospora, (in the Press).Google Scholar
Lissouba, P. & Rizet, G. (1960). Sur l'existence d'une unité génétique polarisée ne subissant que des échanges non réciproques. C. r. hebd. Séanc. Acad. Sci., Paris 250, 3408–3410.Google Scholar
Murray, N. E. (1961). Polarized recombination within the me-2 gene of Neurospora (Abstr.). Genetics, Princeton 46, 886.Google Scholar
Murray, N. E. (1965). Cysteine mutant strains of Neurospora. Genetics, Princeton 52, 801–808.Google Scholar
Murray, N. E. (1969). Reversal of polarized recombination of alleles in Neurospora as a function of their position. Genetics, Princeton 61, 67–77.CrossRefGoogle Scholar
Paszewski, A. (1967). A study on simultaneous conversions in linked genes in Ascobolus immersus. Genet. Res., Camb. 10, 121–126.Google Scholar
Perkins, D. D. (1959). New markers and multiple point linkage data in Neurospora. Genetics, Princeton 44, 1185–1208.CrossRefGoogle Scholar
Putrament, A. (1967). On the mechanism of mitotic recombination in Aspergillus nidulans. II. Simultaneous recombination within two closely linked cistrons. Molec. gen. Genetics 100, 321–336.Google Scholar
Rossignol, J. L. (1964). Phénomènes de recombinaison intragénique et unité fonctionnelle d'un locus chez l'Ascobolus immersus. Thesis, University of Paris.Google Scholar
Seale, T. (1968). Reversion of the am locus in Neurospora; Evidence for nonsense suppression. Genetics, Princeton 58, 85–99.Google Scholar
Siddiqi, O. H. (1961). Polarized replication as a possible condition for recombination (Abstr.). Heredity 16, 23.Google Scholar
Stadler, D. R. & Towe, A. M. (1968). A test of coincident recombination in closely linked genes of Neurospora. Genetics, Princeton 58, 327–336.CrossRefGoogle Scholar
Stahl, F. W. & Murray, N. E. (1966). The evolution of gene clusters and genetic circularity in microorganisms. Genetics, Princeton 53, 569–576.Google Scholar
Whitehouse, H. L. K. (1966). An operator model of crossing over. Nature, Lond. 211, 708–713.Google Scholar
Whitehouse, H. L. K. & HASTINGS, P. J. (1965). The analysis of genetic recombination on the polaron hybrid DNA model Genet. Res., Camb. 6, 27–92.Google Scholar