Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-28T01:21:13.407Z Has data issue: false hasContentIssue false
  • English
  • Français

Rates of spontaneous mitotic recombination in Saccharomyces cerevisiae

Published online by Cambridge University Press:  14 April 2009

R. J. Thornton  and
J. R. Johnston
R. J. Thornton
Affiliation:
Department of Applied Microbiology, University of Strathclyde, Glasgow C.1
J. R. Johnston
Affiliation:
Department of Applied Microbiology, University of Strathclyde, Glasgow C.1
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The rates of mitotic recombination at the loci ade3, ade5–7, ade6 and ade8 were approximated by the method of ‘increasing proportion of variants with growth’. The values (per cell generation) were 3·5 × 10−4 for ade8, 1·4 × 10−4 for ade5–7, 4·0 × 10−5 for ade3 and < 2 × 10−6 for ade6. The relative frequencies of mitotic recombination in regions between ade 5–7 and the centromere were different from those obtained with radiation-induced recombinants.

Type
Research Article
Information
Genetics Research , Volume 18 , Issue 2 , October 1971 , pp. 147 - 151
Copyright
Copyright © Cambridge University Press 1971

References

REFERENCES

Hawthorne, D. C. & Mortimer, R. K. (1960). Chromsome mapping in Saccharomyces: Centromere-linked genes. Genetics 45, 1085–1110.CrossRefGoogle Scholar
Hurst, D. D. & Fogel, S. (1964). Mitotic recombination and heteroallelic repair in Saccharomyces cerevisiae. Genetics 50, 435–458.CrossRefGoogle ScholarPubMed
James, A. P. & Lee-Whiting, B. (1955). Radiation-induced genetic segregation in vegetative cells of yeast. Genetics 40, 826–831.CrossRefGoogle ScholarPubMed
Johnston, J. R. (1962). Studies in meiotic and mitotic recombination in Saccharomyces cerevisiae. Ph.D. thesis, University of California, Berkeley.Google Scholar
Johnston, J. R. & Mackinnon, J. M. (1966). Mitotic segregation of ade 8 in diploid and tetraploid Saccharomyces. Proceedings of 2nd International Symposium on Yeasts, Bratislava, pp. 277–285.Google Scholar
Nakai, S. & Mortimer, R. K. (1969). Studies of the genetic mechanism of radiation-induced mitotic segregation in yeast. Molecular and General Genetics 103, 329–338.CrossRefGoogle ScholarPubMed
Novick, A. & Szilard, L. (1950). Experiments with the chemostat on spontaneous mutations of bacteria. Proceedings of National Academy of Sciencies of the U.S.A. 36, 708–719.CrossRefGoogle ScholarPubMed
Ogur, M. & St John, R. (1956). A differential and diagnostic plating method for population studies of respiration deficiency in yeast. Journal of Bacteriology 72, 500–504.CrossRefGoogle ScholarPubMed
Roman, H. (1956). A system selective for mutations affecting the synthesis of adenine in yeast. Compte rendu Travaux Laboratoire Carlsberg (ser. Physiol.) 26, 299–314.Google Scholar
Stocker, B. A. D. (1949). Measurements of rate of mutation of flagellar antigenic phase in Salmonella typhimurium. Journal of Hygiene, Cambridge 47, 398–413.Google Scholar
Thornton, R. J. & Johnston, J. R. (1970). Mitotic recombination in Saccharomyces. (Abstract.) Heredity 25, 495.Google Scholar
Wilkie, D. & Lewis, D. (1963). The effect of ultraviolet light on recombination in yeast. Genetics 48, 1701–1716.CrossRefGoogle ScholarPubMed
Zimmermann, F. K., Schwaier, R. & Laer, V.U. (1966). Mitotic recombination induced in Saccharomyces cerevisiae with nitrous acid, diethylsulphate and carcinogenic, alkylating nitrosamides. Zeitschrift fü Vererbungslehre 98, 230–246.Google Scholar